Any feedback?
Literature summary extracted from
- Structural, mechanistic, and functional insights into an Arthrobacter nicotinovorans molybdenum hydroxylase involved in nicotine degradation (2021), Molecules, 26, 4387 .
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.5.99.14 | structure of the Kdh holoenzyme, with its three subunits KdhL, KdhM, and KdhS, and their associated cofactors molybdopterin cytosine dinucleotide, two iron-sulfur clusters (Fe2S2), and flavin adenine dinucleotide, respectively. The residues Glu345, Try551, and Glu748 of KdhL participate in substrate binding, and Phe269 and Arg383 of KdhL contribute to stabilize the molybdopterin cytosine dinucleotide conformation | Paenarthrobacter nicotinovorans |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.5.99.14 | E345A | mutation in subunit KdhL, inactive | Paenarthrobacter nicotinovorans |
| 1.5.99.14 | E748A | mutation in subunit KdhL, inactive | Paenarthrobacter nicotinovorans |
| 1.5.99.14 | F269A | mutation in subunit KdhL, 8fold decrease in catalytic efficiency | Paenarthrobacter nicotinovorans |
| 1.5.99.14 | R383A | mutation in subunit KdhL, 2fold decrease in catalytic efficiency | Paenarthrobacter nicotinovorans |
| 1.5.99.14 | W551A | mutation in subunit KdhL, inactive | Paenarthrobacter nicotinovorans |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.99.14 | 0.0125 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, pH 8.0, 30°C | Paenarthrobacter nicotinovorans |  |
| 1.5.99.14 | 0.0125 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, PBS, pH 8.0, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 0.0151 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, Tris/HCl, pH 8.5, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 0.0159 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, Tris/HCl, pH 8.0, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 0.0214 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | mutant R383A, pH 8.0, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 0.0223 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, Tris/HCl, pH 7.4, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 0.0234 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, PBS, pH 7.4, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 0.026 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | mutant F269A, pH 8.0, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 0.0377 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, Tris/HCl, pH 6.8, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 0.065 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, PBS, pH 6.4, 30°C | Paenarthrobacter nicotinovorans | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.99.14 | Paenarthrobacter nicotinovorans | O87681 and O87682 and Q933N0 | O87681 i.e. subunit KdhA/KdhM, O87682 i.e. subunit KdhB/KdhS, Q933N0 i.e. sunbunit KdhC/KdhL | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.99.14 | 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + phenazine methosulfate + H2O | - |
Paenarthrobacter nicotinovorans | 1-(2,6-dihydroxypyridin-3-yl)-4-(methylamino)butan-1-one + reduced phenazine methosulfate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.99.14 | KdhA | - |
Paenarthrobacter nicotinovorans |
| 1.5.99.14 | KdhB | - |
Paenarthrobacter nicotinovorans |
| 1.5.99.14 | KdhC | - |
Paenarthrobacter nicotinovorans |
| 1.5.99.14 | KdhL | - |
Paenarthrobacter nicotinovorans |
| 1.5.99.14 | KdhM | - |
Paenarthrobacter nicotinovorans |
| 1.5.99.14 | KdhS | - |
Paenarthrobacter nicotinovorans |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.99.14 | 2.3 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | mutant F269A, pH 8.0, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 5.13 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, PBS, pH 6.4, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 6.3 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | mutant R383A, pH 8.0, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 8.92 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, Tris/HCl, pH 8.5, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 9.03 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, pH 8.0, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 9.03 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, PBS, pH 8.0, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 9.09 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, Tris/HCl, pH 6.8, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 9.28 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, Tris/HCl, pH 7.4, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 9.47 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, Tris/HCl, pH 8.0, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 10.58 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, PBS, pH 7.4, 30°C | Paenarthrobacter nicotinovorans | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.99.14 | FAD | - |
Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | iron-sulfur centre | presence of two [Fe2-S2] centers | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | molybdopterin cytosine dinucleotide | - |
Paenarthrobacter nicotinovorans | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.99.14 | 80 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, PBS, pH 6.4, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 90 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | mutant F269A, pH 8.0, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 240 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, Tris/HCl, pH 6.8, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 300 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | mutant R383A, pH 8.0, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 420 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, Tris/HCl, pH 7.4, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 450 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, PBS, pH 7.4, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 590 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, Tris/HCl, pH 8.5, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 600 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, Tris/HCl, pH 8.0, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 720 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, pH 8.0, 30°C | Paenarthrobacter nicotinovorans | |
| 1.5.99.14 | 720 | - |
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one | wild-type, PBS, pH 8.0, 30°C | Paenarthrobacter nicotinovorans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
