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Literature summary extracted from

  • Gibbons, A.E.; Luker, K.E.; Luker, G.D.
    Dual reporter bioluminescence imaging with nanoLuc and firefly luciferase (2018), Methods Mol. Biol., 1790, 41-50 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.13.12.13 molecular biology bioluminescence imaging is a powerful, broadly utilized method for non-invasive imaging studies in cell-based assays and small animal models of normal physiology and multiple diseases. In combination with molecular engineering of cells and entire organisms using luciferase enzymes, bioluminescence imaging has enabled novel applications including studies of protein-protein interactions, ligand-receptor interactions, cell trafficking, and drug targeting in mouse models. The use of a luciferase enzyme derived from Oplophorus gracilirostris, NanoLuc, is described in cell-based assays bioluminescence imaging of tumor-bearing mice. NanoLuc is combined with another luciferase enzyme, firefly luciferase, to image multiple signal transduction events in one imaging session Oplophorus gracilirostris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.13 Oplophorus luciferin + O2 Oplophorus gracilirostris
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 oxidized Oplophorus luciferin + CO2 + hv
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.13 Oplophorus gracilirostris Q9GV45 AND Q9GV46 Q9GV45: catalytic subunit, Q9GV46: non-catalytic subunit
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.13 Oplophorus luciferin + O2
-
 Oplophorus gracilirostris oxidized Oplophorus luciferin + CO2 + hv
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.13 NanoLuc
-
 Oplophorus gracilirostris