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Literature summary extracted from

  • Zeng, X.H.; Du, H.; Zhao, H.M.; Xiang, L.; Feng, N.X.; Li, H.; Li, Y.W.; Cai, Q.Y.; Mo, C.H.; Wong, M.H.; He, Z.L.
    Insights into the binding interaction of substrate with catechol 2,3-dioxygenase from biophysics point of view (2020), J. Hazard. Mater., 391, 122211 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 expression in Escherichia coli BL21 (DE3) Rhodococcus sp. 2G

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.2 E270A mutant enzyme is almost completely inactivated Rhodococcus sp. 2G
1.13.11.2 H157A mutant enzyme is almost completely inactivated Rhodococcus sp. 2G
1.13.11.2 H202A mutant enzyme is almost completely inactivated Rhodococcus sp. 2G
1.13.11.2 H219A mutant enzyme is almost completely inactivated Rhodococcus sp. 2G
1.13.11.2 T254A catalytic activity of the mutant enzyme hardly changes. It shows a broader substrate specificity than the wild-type enzyme. The activities of the mutant enzyme on 3-methylcatechol and 4-methylcatechol increases 3.7fold and 2.5folds, respectively, as compared to catechol Rhodococcus sp. 2G

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.03935
-
 catechol pH 7.5, 30°C Rhodococcus sp. 2G

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.2 41000
-
 calculated from sequence Rhodococcus sp. 2G
1.13.11.2 47000
-
 SDS-PAGE Rhodococcus sp. 2G

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 catechol + O2 Rhodococcus sp. 2G
-
 cis,cis-muconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Rhodococcus sp. 2G A0A1J0TVF4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.2
-
 Rhodococcus sp. 2G

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3-methylcatechol + O2 wild-type enzyme shows 6.1% of the activity as compared to catechol. The activities of the mutant enzyme T254A increases 3.7fold as compared to catechol Rhodococcus sp. 2G 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 4-chlorocatechol + O2 19.2% of the activity as compared to catechol Rhodococcus sp. 2G 5-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2 wild-type enzyme shows 92.1% of the activity as compared to catechol. The activities of the mutant enzyme T254A increases 2.5fold as compared to catechol Rhodococcus sp. 2G 2-hydroxy-5-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 catechol + O2
-
 Rhodococcus sp. 2G cis,cis-muconate
-
 ?
1.13.11.2 catechol + O2
-
 Rhodococcus sp. 2G 2-hydroxymuconate-6-semialdehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.2 C23O-2G
-
 Rhodococcus sp. 2G

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.2 30
-
-
 Rhodococcus sp. 2G

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.2 7.5
-
-
 Rhodococcus sp. 2G