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Literature summary extracted from
- Purification and characterization of peroxidases from garden cress sprouts and their roles in lignification and removal of phenol and p-chlorophenol (2021), J. Food Biochem., 45, e13526 .
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.7 | Co2+ | 5 mM, 79% inhibition of cationic peroxidase GCP1. 5 mM, 20% inhibition of anionic peroxidase GCP2 | Lepidium sativum |  |
| 1.11.1.7 | Cu2+ | 5 mM, 85% inhibition of cationic peroxidase GCP1. 5 mM, 26% inhibition of anionic peroxidase GCP2 | Lepidium sativum | |
| 1.11.1.7 | Hg2+ | 5 mM, 93% inhibition of cationic peroxidase GCP1. 5 mM, 33% inhibition of anionic peroxidase GCP2 | Lepidium sativum | |
| 1.11.1.7 | Mn2+ | 5 mM, 41% inhibition of cationic peroxidase GCP1 | Lepidium sativum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.7 | 1.1 | - |
H2O2 | pH 6.0-7.5, 30°C, cationic peroxidase GCP1 | Lepidium sativum | |
| 1.11.1.7 | 1.2 | - |
guaiacol | pH 6.0-7.5, 30°C, cationic peroxidase GCP1 | Lepidium sativum | |
| 1.11.1.7 | 1.6 | - |
H2O2 | pH 7.0, 40°C, anionic peroxidase GCP2 | Lepidium sativum | |
| 1.11.1.7 | 3.6 | - |
guaiacol | pH 7.0, 40°C, anionic peroxidase GCP2 | Lepidium sativum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.7 | Ba2+ | 5 mM, 1.13fold activation of cationic peroxidase GCP1, 1.3fold activation of anionic peroxidase GCP2 | Lepidium sativum | |
| 1.11.1.7 | Ca2+ | 5 mM, 1.19fold activation of cationic peroxidase GCP1, 1.4fold activation of anionic peroxidase GCP2 | Lepidium sativum | |
| 1.11.1.7 | Mg2+ | 5 mM, 1.42fold activation of anionic peroxidase GCP2 | Lepidium sativum | |
| 1.11.1.7 | Mn2+ | 5 mM, 1.27fold activation of anionic peroxidase GCP2 | Lepidium sativum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.7 | 25000 | - |
gel filtration, cationic peroxidase GCP1 | Lepidium sativum |
| 1.11.1.7 | 40000 | - |
gel filtration, anionic peroxidase GCP2 | Lepidium sativum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.7 | Lepidium sativum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.11.1.7 | - |
Lepidium sativum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.11.1.7 | sprout | - |
Lepidium sativum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.7 | benzoic acid + H2O2 | 27% compared to the activity with guaiacol, anionic peroxidase GCP2 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | benzoic acid + H2O2 | activity is 2.13fold higher than activity with guaiacol, cationic peroxidase GCP1 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | catechin + H2O2 | 1.7% compared to the activity with guaiacol, cationic peroxidase GCP1 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | catechin + H2O2 | 3.5% compared to the activity with guaiacol, anionic peroxidase GCP2 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | catechol + H2O2 | 4.5% compared to the activity with guaiacol, cationic peroxidase GCP1 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | catechol + H2O2 | 5.1% compared to the activity with guaiacol, anionic peroxidase GCP2 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | cinnamic acid + H2O2 | 15% compared to the activity with guaiacol, cationic peroxidase GCP1 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | cinnamic acid + H2O2 | activity is 1.18fold higher than activity with guaiacol, anionic peroxidase GCP2 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | coniferyl alcohol + H2O2 | 18% compared to the activity with guaiacol, cationic peroxidase GCP1 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | coniferyl alcohol + H2O2 | activity is 1.27fold higher than activity with guaiacol, anionic peroxidase GCP2 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | ferulic acid + H2O2 | 17% compared to the activity with guaiacol, cationic peroxidase GCP1 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | ferulic acid + H2O2 | 83.3% compared to the activity with guaiacol, anionic peroxidase GCP2 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | guaiacol + H2O2 | - |
Lepidium sativum | tetraguaiacol + H2O | - |
? | |
| 1.11.1.7 | m-phenylenediamine + H2O2 | 12% compared to the activity with guaiacol, cationic peroxidase GCP1 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | m-phenylenediamine + H2O2 | 13.5% compared to the activity with guaiacol, anionic peroxidase GCP2 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | o-dianisidine + H2O2 | 25% compared to the activity with guaiacol, anionic peroxidase GCP2 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | o-dianisidine + H2O2 | 45% compared to the activity with guaiacol, cationic peroxidase GCP1 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | o-phenylenediamine + H2O2 | activity is 2.37fold higher than activity with guaiacol, anionic peroxidase GCP2 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | o-phenylenediamine + H2O2 | activity is 3.14fold higher than activity with guaiacol, cationic peroxidase GCP1 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | p-aminoantipyrine + H2O2 | 26.5% compared to the activity with guaiacol, cationic peroxidase GCP1 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | p-aminoantipyrine + H2O2 | 31% compared to the activity with guaiacol, anionic peroxidase GCP2 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | p-hydroxybenzoic acid + H2O2 | 5.0% compared to the activity with guaiacol, anionic peroxidase GCP2 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | p-hydroxybenzoic acid + H2O2 | activity is 1.41fold higher than activity with guaiacol, cationic peroxidase GCP1 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | pyrogallol + H2O2 | 7.4% compared to the activity with guaiacol, anionic peroxidase GCP2 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | pyrogallol + H2O2 | 88% compared to the activity with guaiacol, cationic peroxidase GCP1 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | sinapyl alcohol + H2O2 | 30% compared to the activity with guaiacol, cationic peroxidase GCP1 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | sinapyl alcohol + H2O2 | activity is 1.32fold higher than activity with guaiacol, anionic peroxidase GCP2 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | tannic acid + H2O2 | 3.8% compared to the activity with guaiacol, cationic peroxidase GCP1 | Lepidium sativum | ? + H2O | - |
? | |
| 1.11.1.7 | tannic acid + H2O2 | 78% compared to the activity with guaiacol, anionic peroxidase GCP2 | Lepidium sativum | ? + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.11.1.7 | monomer | 1 * 25000, SDS-PAGE, cationic peroxidase GCP1 | Lepidium sativum |
| 1.11.1.7 | monomer | 1 * 40000, gel filtration, anionic peroxidase GCP2 | Lepidium sativum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.11.1.7 | GCP1 | - |
Lepidium sativum |
| 1.11.1.7 | GCP2 | - |
Lepidium sativum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.7 | 30 | - |
cationic peroxidase GCP1 | Lepidium sativum |
| 1.11.1.7 | 40 | - |
anionic peroxidase GCP2 | Lepidium sativum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.7 | 20 | 60 | 20°C: about 95% of maximal activity, 60°C: about 50% of maximal activity, cationic peroxidase GCP1 | Lepidium sativum |
| 1.11.1.7 | 20 | 70 | 20°C: about 95% of maximal activity, 70°C: about 55% of maximal activity, anionic peroxidase GCP2 | Lepidium sativum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.7 | 50 | - |
30 min, cationic peroxidase GCP1 and anionic peroxidase GCP2 retain 100% of their activities | Lepidium sativum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.7 | 6 | 7.5 | cationic peroxidase GCP1 | Lepidium sativum |
| 1.11.1.7 | 7 | - |
anionic peroxidase GCP2 | Lepidium sativum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.7 | 4 | 8 | pH 4.0: about 35% of maximal activity, pH 8.0: about 60% of maximal activity, anionic peroxidase GCP2 | Lepidium sativum |
| 1.11.1.7 | 4.5 | 8.5 | pH 4.5: about 40% of maximal activity, pH 8.5: about 45% of maximal activity, cationic peroxidase GCP1 | Lepidium sativum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.7 | 5.5 | 8 | 2 h, no loss of activity, cationic peroxidase CGP1 | Lepidium sativum |
| 1.11.1.7 | 6 | 7.5 | 2 h, no loss of activity, anionic peroxidase CGP2 | Lepidium sativum |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.11.1.7 | physiological function | important antioxidant enzyme, which is elevated when plants are subjected to pollution. GCP2 may play an important role in lignification process and removal of phenol and p-chlorophenol from polluted soil/wastewater. It resists the harmful effect of heavy metals. Cationic peroxidase GCP1, as a natural scavenger, has high affinity toward H2O2 coupled to oxidation of some plant phenolic compounds suggesting its role in consuming of excess H2O2 | Lepidium sativum |
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