BRENDA - Enzyme Database show

Kinetic studies on the specificity of long chain acyl coenzyme A synthetase from rat liver microsomes

Marcel, Y.L.; Suzue, G.; J. Biol. Chem. 247, 4433-4436 (1972)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.3
(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoic acid
competitive inhibitor of palmitoleic acid activation
Rattus norvegicus
6.2.1.3
Eicosa-11,14,17-trienoic acid
competitive inhibitor of palmitoleic acid activation
Rattus norvegicus
6.2.1.3
eicosa-8,11,14-trienoic acid
competitive inhibitor of palmitoleic acid activation
Rattus norvegicus
6.2.1.3
Eicosa-8,14-dienoic acid
competitive inhibitor of palmitoleic acid activation
Rattus norvegicus
6.2.1.3
palmitate
linolenic acid activation
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.2.1.3
0.00139
-
oleate
-
Rattus norvegicus
6.2.1.3
0.00164
-
linolenate
-
Rattus norvegicus
6.2.1.3
0.00204
-
palmitoleate
-
Rattus norvegicus
6.2.1.3
0.00222
-
linoleate
-
Rattus norvegicus
6.2.1.3
0.00278
-
palmitate
-
Rattus norvegicus
6.2.1.3
0.0072
-
CoA
activation of palmitic acid
Rattus norvegicus
6.2.1.3
0.0094
-
CoA
activation of oleic acid
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.2.1.3
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.2.1.3
Mg2+
required
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.2.1.3
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.2.1.3
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.3
ATP + linoleate + CoA
-
765
Rattus norvegicus
AMP + diphosphate + linoleoyl-CoA
-
-
-
-
6.2.1.3
ATP + linolenate + CoA
-
765
Rattus norvegicus
AMP + diphosphate + linolenoyl-CoA
-
-
-
-
6.2.1.3
ATP + oleate + CoA
-
765
Rattus norvegicus
AMP + diphosphate + oleoyl-CoA
-
-
-
-
6.2.1.3
ATP + palmitate + CoA
-
765
Rattus norvegicus
AMP + diphosphate + palmitoyl-CoA
-
-
-
-
6.2.1.3
ATP + palmitoleate + CoA
-
765
Rattus norvegicus
AMP + diphosphate + palmitoleoyl-CoA
-
-
-
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.2.1.3
45
-
half-life: 15 min
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.3
(5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoic acid
competitive inhibitor of palmitoleic acid activation
Rattus norvegicus
6.2.1.3
Eicosa-11,14,17-trienoic acid
competitive inhibitor of palmitoleic acid activation
Rattus norvegicus
6.2.1.3
eicosa-8,11,14-trienoic acid
competitive inhibitor of palmitoleic acid activation
Rattus norvegicus
6.2.1.3
Eicosa-8,14-dienoic acid
competitive inhibitor of palmitoleic acid activation
Rattus norvegicus
6.2.1.3
palmitate
linolenic acid activation
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.2.1.3
0.00139
-
oleate
-
Rattus norvegicus
6.2.1.3
0.00164
-
linolenate
-
Rattus norvegicus
6.2.1.3
0.00204
-
palmitoleate
-
Rattus norvegicus
6.2.1.3
0.00222
-
linoleate
-
Rattus norvegicus
6.2.1.3
0.00278
-
palmitate
-
Rattus norvegicus
6.2.1.3
0.0072
-
CoA
activation of palmitic acid
Rattus norvegicus
6.2.1.3
0.0094
-
CoA
activation of oleic acid
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.2.1.3
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.2.1.3
Mg2+
required
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.2.1.3
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.3
ATP + linoleate + CoA
-
765
Rattus norvegicus
AMP + diphosphate + linoleoyl-CoA
-
-
-
-
6.2.1.3
ATP + linolenate + CoA
-
765
Rattus norvegicus
AMP + diphosphate + linolenoyl-CoA
-
-
-
-
6.2.1.3
ATP + oleate + CoA
-
765
Rattus norvegicus
AMP + diphosphate + oleoyl-CoA
-
-
-
-
6.2.1.3
ATP + palmitate + CoA
-
765
Rattus norvegicus
AMP + diphosphate + palmitoyl-CoA
-
-
-
-
6.2.1.3
ATP + palmitoleate + CoA
-
765
Rattus norvegicus
AMP + diphosphate + palmitoleoyl-CoA
-
-
-
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.2.1.3
45
-
half-life: 15 min
Rattus norvegicus