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Literature summary extracted from

  • Adriani, P.P.; de Paiva, F.C.R.; de Oliveira, G.S.; Leite, A.C.; Sanches, A.S.; Lopes, A.R.; Dias, M.V.B.; Chambergo, F.S.
    Structural and functional characterization of the glutathione peroxidase-like thioredoxin peroxidase from the fungus Trichoderma reesei (2021), Int. J. Biol. Macromol., 167, 93-100 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 expressed in Escherichia coli BL21(DE3) cells Trichoderma reesei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.24 hanging drop vapor diffusion method, using 30% (w/v) PEG 400, 200 mM MgCl2, and 100 mM HEPES pH 7.5 Trichoderma reesei
1.11.1.24 structure in a reduced state and non-catalytic conformation. The overall fold is similar to the other phospholipid-hydroperoxide glutathione peroxidases Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.24 1.6
-
 H2O2 at pH 7.2 and 30°C Trichoderma reesei
1.11.1.24 11.7
-
 H2O2 pH 8, 25°C Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Trichoderma reesei G0RHF8
-
-
1.11.1.24 Trichoderma reesei QM6a G0RHF8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24 Sepharose column chromatography, and Superdex 200 gel filtration Trichoderma reesei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.24 12.5
-
 substrate tert-butyl hydroperoxide, pH 8, 25°C Trichoderma reesei
1.11.1.24 28
-
 substrate H2O2, pH 8, 25°C Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 2 thioredoxin + H2O2
-
 Trichoderma reesei thioredoxin disulfide + 2 H2O
-
 ?
1.11.1.24 2 thioredoxin + H2O2
-
 Trichoderma reesei QM6a thioredoxin disulfide + 2 H2O
-
 ?
1.11.1.24 2 thioredoxin + tert-butyl hydroperoxide
-
 Trichoderma reesei thioredoxin disulfide + H2O + tert-butyl alcohol
-
 ?
1.11.1.24 2 thioredoxin + tert-butyl hydroperoxide
-
 Trichoderma reesei QM6a thioredoxin disulfide + H2O + tert-butyl alcohol
-
 ?
1.11.1.24 H2O2 + pyrogallol
-
 Trichoderma reesei ?
-
 ?
1.11.1.24 H2O2 + pyrogallol
-
 Trichoderma reesei QM6a ?
-
 ?
1.11.1.24 H2O2 + reduced thioredoxin highest activity Trichoderma reesei oxidized thioredoxin + H2O
-
 ?
1.11.1.24 additional information no activity with glutathione Trichoderma reesei ?
-
-
1.11.1.24 additional information no activity with glutathione Trichoderma reesei QM6a ?
-
-
1.11.1.24 tert-butyl hydroperoxide + reduced thioredoxin
-
 Trichoderma reesei tert-butanol + oxidized thioredoxin + H2O
-
 ?
1.11.1.24 tert-butyl hydroperoxide + reduced thioredoxin
-
 Trichoderma reesei QM6a tert-butanol + oxidized thioredoxin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 ? x * 19500, calculated from sequence Trichoderma reesei
1.11.1.24 homodimer 2 * 19500, calculated from amino acid sequence Trichoderma reesei

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 GPX
-
 Trichoderma reesei
1.11.1.24 thioredoxin peroxidase
-
 Trichoderma reesei
1.11.1.24 TRIREDRAFT_47136
-
 Trichoderma reesei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.24 80
-
 half-life 36 min Trichoderma reesei
1.11.1.24 80
-
 half-life of 36 min at 80°C Trichoderma reesei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.24 19
-
 H2O2 pH 8, 25°C Trichoderma reesei
1.11.1.24 19
-
 H2O2 at pH 7.2 and 30°C Trichoderma reesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.24 9 12 broad Trichoderma reesei
1.11.1.24 10
-
-
 Trichoderma reesei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.24 9 12 the enzyme shows best activity between pH 9.0 and 12.0 Trichoderma reesei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.24 Trichoderma reesei calculated from amino acid sequence
-
 5.6
1.11.1.24 Trichoderma reesei calculated from sequence
-
 5.6

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.24 1.6
-
 H2O2 pH 8, 25°C Trichoderma reesei
1.11.1.24 11.7
-
 H2O2 at pH 7.2 and 30°C Trichoderma reesei