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Literature summary extracted from

  • Wang, H.; Huang, L.; Li, Y.; Ma, J.; Wang, S.; Zhang, Y.; Ge, X.; Wang, N.; Lu, F.; Liu, Y.
    Characterization and application of a novel laccase derived from Bacillus amyloliquefaciens (2020), Int. J. Biol. Macromol., 150, 982-990 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 environmental protection the enzyme is an ideal candidate for lots of biotechnological and industrial applications due to its stability in the extreme conditions Bacillus amyloliquefaciens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 expression in Escherichia coli Bacillus amyloliquefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 2-mercaptoethanol 0.1 mM, 65.5% inhibition Bacillus amyloliquefaciens
1.10.3.2 BaCl2 5 mM, 10% inhibition Bacillus amyloliquefaciens
1.10.3.2 CoCl2 5 mM, 19% inhibition Bacillus amyloliquefaciens
1.10.3.2 DTT 0.1 mM, complete inhibition Bacillus amyloliquefaciens
1.10.3.2 EDTA 5 mM, 51.5% inhibition Bacillus amyloliquefaciens
1.10.3.2 FeCl3 5 mM, 14% loss of activity Bacillus amyloliquefaciens
1.10.3.2 FeSO4 5 mM, complete loss of activity Bacillus amyloliquefaciens
1.10.3.2 L-cysteine 0.5 mM, 67% inhibition Bacillus amyloliquefaciens
1.10.3.2 MnCl2 5 mM, 90% loss of activity Bacillus amyloliquefaciens
1.10.3.2 NaCl 10 mM, 20% inhibition. 500 mM, 63% inhibition. 1000 mM, complete loss of activity Bacillus amyloliquefaciens
1.10.3.2 NiSO4 5 mM, 4% inhibition Bacillus amyloliquefaciens
1.10.3.2 SDS 5 mM, 17% inhibition Bacillus amyloliquefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 copper copper-containing enzyme Bacillus amyloliquefaciens
1.10.3.2 MgCl2 0.5 mM, 1.08fold activation Bacillus amyloliquefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 58000
-
 calculated from sequence, SDS-PAGE Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Bacillus amyloliquefaciens A0A6C0GY11
-
-
1.10.3.2 Bacillus amyloliquefaciens TCCC 111018 A0A6C0GY11
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Bacillus amyloliquefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Bacillus amyloliquefaciens ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Bacillus amyloliquefaciens TCCC 111018 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Bacillus amyloliquefaciens ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Bacillus amyloliquefaciens TCCC 111018 ?
-
 ?
1.10.3.2 Adizol black B + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
1.10.3.2 Adizol black B + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens TCCC 111018 ?
-
 ?
1.10.3.2 Azophloxine + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
1.10.3.2 Azophloxine + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens TCCC 111018 ?
-
 ?
1.10.3.2 Congo Red + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
1.10.3.2 Congo Red + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens TCCC 111018 ?
-
 ?
1.10.3.2 Crystal violet + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
1.10.3.2 indigo carmine + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
1.10.3.2 Malachite Green + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
1.10.3.2 Reactive blue 19 + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
1.10.3.2 Reactive blue 5 + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?
1.10.3.2 Remazol Brilliant Blue R + O2 decolorization with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetosyringone or syringaldehyde as mediator Bacillus amyloliquefaciens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 CotA-laccase
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 80
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) or 2,6-dimethoxyphenol Bacillus amyloliquefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 60
-
 stable up to when using the substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 5.5
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Bacillus amyloliquefaciens
1.10.3.2 7
-
 substrate: 2,6-dimethoxyphenol Bacillus amyloliquefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 4 7 pH 4.0: about 35% of maximal activity, pH 7.0: about 55% of maximal activity, substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Bacillus amyloliquefaciens
1.10.3.2 6 9 pH 6.0: about 75% of maximal activity, pH 9.0: about 50% of maximal activity, substrate: 2,6-dimethoxyphenol Bacillus amyloliquefaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 3 9 stable up to when using the substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Bacillus amyloliquefaciens