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Literature summary extracted from

  • Zeinoddini, M.; Fathi-Roudsari, M.; Hosseinkhani, S.; Khajeh, K.
    Bioluminescence and kinetic aspects of double mutated aequorin variants (2018), Int. J. Biol. Macromol., 112, 163-168 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.24 expression in Escherichia coli BL-21(DE3) Aequorea victoria

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.24 D153G shift of maximum luminescence to shorter wavelengths. t1/2 is decreased with respect to the half-life time of the native protein (0.7 s). Mutation results in a significant decrease in stability at 65°C Aequorea victoria
1.13.12.24 W86F shift of maximum luminescence to shorter wavelengths. The single mutant reaches the highest stability against thermal shock Aequorea victoria
1.13.12.24 W86F/D153G decreased t1/2-value. Maximum emission spectrum at 401 nm. Mutation results in a significant decrease in stability at 65°C Aequorea victoria
1.13.12.24 Y82F mutation shifts the peak towards longer wavelengths. Increased half time of initial light by 3.6 s. Mutation results in a significant decrease in stability at 65°C Aequorea victoria
1.13.12.24 Y82F/D153G decreased t1/2-value. Maximum emission spectrum at 478 nm Aequorea victoria
1.13.12.24 Y82F/W86F maximum emission spectrum at 400 nm. Y82F mutation results in shift of emission to longer wavelength, while the W86F mutation shifts the emission to shorter wavelengths. Compared to wild type aequorin, the Y82F/W86F variant displays a 2fold increase of light half-life. Mutation results in a significant decrease in stability at 65°C Aequorea victoria

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.24 Ca2+ calcium-regulated photoprotein Aequorea victoria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.24 26000
-
 SDS-PAGE Aequorea victoria

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.24 Aequorea victoria P02592
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.24
-
 Aequorea victoria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.24 [apoaequorin] + coelenterazine + O2 + 3 Ca2+ flash type light emission Aequorea victoria [excited state blue fluorescent protein] + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.12.24 ? x * 26000, SDS-PAGE Aequorea victoria