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Literature summary extracted from

  • Hassan, H.A.; Aly, A.A.
    Isolation and characterization of three novel catechol 2,3-dioxygenase from three novel haloalkaliphilic BTEX-degrading Pseudomonas strains (2017), Int. J. Biol. Macromol., 106, 1107 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.13.11.2 degradation strain is able to degrade a solution containing benzene, toluene, ethylbenzene, and xylene at 7% NaCl (w/v) and pH 9 Pseudomonas sp. HA10
1.13.11.39 degradation strain is able to degrade a solution containing benzene, toluene, ethylbenzene, and xylene at 7% NaCl (w/v) and pH 9 Pseudomonas sp. HA10

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.2 3-chlorocatechol rapid inactivation Pseudomonas sp. HA10
1.13.11.39 3-chlorocatechol rapid inactivation Pseudomonas sp. HA10

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.035
-
 3-methylcatechol pH not specified in the publication, temperature not specified in the publication Pseudomonas sp. HA10
1.13.11.2 0.4
-
 catechol pH not specified in the publication, temperature not specified in the publication Pseudomonas sp. HA10
1.13.11.39 0.0015
-
 biphenyl-2,3-diol pH not specified in the publication, temperature not specified in the publication Pseudomonas sp. HA10

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Pseudomonas sp. HA10
-
-
-
1.13.11.39 Pseudomonas sp. HA10
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3-methylcatechol + O2
-
 Pseudomonas sp. HA10 2-hydroxy-3-methylmuconate-6-semialdehyde
-
 ?
1.13.11.2 catechol + O2
-
 Pseudomonas sp. HA10 2-hydroxymuconate-6-semialdehyde
-
 ?
1.13.11.2 additional information preferred substrates are catechol and 2,3-dihydroxybiphenyl, reaction of EC 1.13.11.39 Pseudomonas sp. HA10 ?
-
-
1.13.11.39 biphenyl-2,3-diol + O2
-
 Pseudomonas sp. HA10 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate
-
 ?
1.13.11.39 additional information preferred substrates are catechol, reaction of EC 1.13.11.2, and 2,3-dihydroxybiphenyl Pseudomonas sp. HA10 ?
-
-

Subunits

EC Number Subunits Comment Organism
1.13.11.2 ? x * 35000, SDS-PAGE Pseudomonas sp. HA10
1.13.11.39 ? x * 35000, SDS-PAGE Pseudomonas sp. HA10

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.2 200
-
 3-methylcatechol pH not specified in the publication, temperature not specified in the publication Pseudomonas sp. HA10
1.13.11.2 400
-
 catechol pH not specified in the publication, temperature not specified in the publication Pseudomonas sp. HA10
1.13.11.39 250
-
 biphenyl-2,3-diol pH not specified in the publication, temperature not specified in the publication Pseudomonas sp. HA10

General Information

EC Number General Information Comment Organism
1.13.11.2 physiological function strain is able to degrade a solution containing benzene, toluene, ethylbenzene, and xylene at 7% NaCl (w/v) and pH 9 Pseudomonas sp. HA10
1.13.11.39 physiological function strain is able to degrade a solution containing benzene, toluene, ethylbenzene, and xylene at 7% NaCl (w/v) and pH 9 Pseudomonas sp. HA10