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Literature summary extracted from

  • Wei, J.; Wang, Y.; Li, X.; Zhang, X.; Liu, Y.
    Mechanistic insights into pyridine ring degradation catalyzed by 2,5-dihydroxypyridine dioxygenase NicX (2022), Inorg. Chem., 61, 2517-2529 .
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.9 Fe2+ mononuclear non-heme iron enzyme Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.9 2,5-dihydroxypyridine + O2 Pseudomonas putida
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 N-formylmaleamic acid
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.9 Pseudomonas putida Q88FY1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.9 2,5-dihydroxypyridine + O2
-
 Pseudomonas putida N-formylmaleamic acid
-
 ?
1.13.11.9 2,5-dihydroxypyridine + O2 a series of combined quantum mechanics and molecular mechanics (QM/MM) calculations is performed to illuminate the catalytic mechanism of 2,5-dihydroxypyridine dioxygenase (NicX). On the basis of the calculations, the most plausible reaction pathway and the structures of transition states and intermediates involved in the reaction, as well as the energy profiles, are described in detail Pseudomonas putida N-formylmaleamic acid
-
 ?
1.13.11.9 2,5-dihydroxypyridine + O2 two key factors may influence the substrate specificity of the enzyme (NicX): 1. the successful binding of substrates in the active center, in which the pocket residues, including His189, His105, and Glu177, play a key role in substrate recognition, 2. the activation of the substrate by electron transfer from the substrate to dioxygen. Based on the crystal structure of the 2,5-dihydroxypyridine dioxygenase (NicX), an enzyme-substrate complex model is constructed and QM/MM calculations are carried out to unravel the mechanism of NicX-catalyzed heterocyclic ring cleavage involved in the biochemical degradation of nicotine Pseudomonas putida N-formylmaleamic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.9 2,5-dihydroxypyridine dioxygenase
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 Pseudomonas putida
1.13.11.9 NicX
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 Pseudomonas putida