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Literature summary extracted from

  • Zhang, K.; Yuan, X.; Zang, J.; Wang, M.; Zhao, F.; Li, P.; Cao, H.; Han, J.; Xing, J.; Dong, J.
    The kynurenine 3-monooxygenase encoding gene, BcKMO, is involved in the growth, development, and pathogenicity of Botrytis cinerea (2018), Front. Microbiol., 9, 1039 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.9 gene BC1G_07455, DNA and amino acid sequence determination and analysis of wild-type and enzyme mutant gene, phylogenetic analysis and tree, recombinant expression of eGFP-tagged wild-type enzyme in Botrytis cinerea mutant strain BCG183 protoplasts, quantitative real-time PCR enzyme expression analysis Botrytis cinerea

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.9 additional information pathogenic mutant BCG183 is obtained by screening the T-DNA insertion library of Botrytis cinerea. Semiquantitative RT-PCR is used to determine the expression levels of the T-DNA insert gene and confirm the presence of the mutant gene in the mutant BCG183. The pathogenicity-related gene BcKMO, which encodes kynurenine 3-monooxygenase (KMO), is isolated and identified via thermal asymmetric interlaced PCR, bioinformatics analyses, and KMO activity measurement. The mutant BCG183 grows slowly, does not produce conidia and sclerotia, has slender hyphae, and presents enhanced pathogenicity. The phenotype and pathogenicity of the BcKMO complementing mutant (BCG183/BcKMO) are similar to those of the wild-type strain. The wild-type and BCG183/BcKMO colonies are taupe-colored and produce large amounts of sclerotia, while mutant the BCG183 colonies are gray and do not produce sclerotia. The BCG183 mycelia are white and slender with shorter transverse septa, when compared with wild-type and BCG183/BcKMO mycelia. The BCG183 mutant do not produce conidia, whereas the wild-type and BCG183/BcKMO strains do. The BCG183 mutant exhibits remarkably higher sensitivity to NaCl and KCl, when compared with the wild-type. Also, the sensitivity of the mutant BCG183 to fluconazole, Congo Red, menadione, and H2O2 is significantly weaker, when compared with that of the wild-type and BCG183/BcKMO strains. The BCG183 mutant sensitivity to SQ22536 and U0126, inhibitors of the cAMP and MAPK signaling pathways, is significantly weaker, when compared with that of wild-type and BCG183/BcKMO strains. The cAMP content in the mutant BCG183 is significantly lower, when compared with that in wild-type and BCG183/BcKMO strains Botrytis cinerea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.9 L-kynurenine + NADPH + H+ + O2 Botrytis cinerea
-
 3-hydroxy-L-kynurenine + NADP+ + H2O
-
 ?
1.14.13.9 L-kynurenine + NADPH + H+ + O2 Botrytis cinerea BC22
-
 3-hydroxy-L-kynurenine + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.9 Botrytis cinerea
-
-
-
1.14.13.9 Botrytis cinerea BC22
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.9 mycelium
-
 Botrytis cinerea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.9 L-kynurenine + NADPH + H+ + O2
-
 Botrytis cinerea 3-hydroxy-L-kynurenine + NADP+ + H2O
-
 ?
1.14.13.9 L-kynurenine + NADPH + H+ + O2
-
 Botrytis cinerea BC22 3-hydroxy-L-kynurenine + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.9 BcKMO
-
 Botrytis cinerea
1.14.13.9 KMO
-
 Botrytis cinerea

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.9 FAD
-
 Botrytis cinerea
1.14.13.9 NADPH
-
 Botrytis cinerea

General Information

EC Number General Information Comment Organism
1.14.13.9 malfunction enzyme BcKMO deficiency reduces the resistance of Bortrytis cinerea to osmotic stress, suggesting a positive regulatory role of this gene in osmotic stress resistance in Bortrytis cinerea. A similar trend is also noted with respect to the inhibition rate. The cellular integrity is enhanced in the mutant BCG183 Botrytis cinerea
1.14.13.9 metabolism BcKMO is involved in cAMP and MAPK signaling pathways Botrytis cinerea
1.14.13.9 physiological function BcKMO is important for growth and development of Bortrytis cinerea. Enzyme BcKMO regulates the activities of cell wall degrading enzymes (CWDEs), toxins, and acid production Botrytis cinerea