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Literature summary extracted from

  • Wang, H.; Zhi, X.; Qiu, J.; Shi, L.; Lu, Z.
    Characterization of a novel nicotine degradation gene cluster ndp in Sphingomonas melonis TY and its evolutionary analysis (2017), Front. Microbiol., 8, 337 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.5 expression in Sphingomonas aquatilis, Escherichia coli strains are not suitable for expression Sphingomonas melonis

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.5 Sphingomonas melonis
-
-
-
1.5.3.5 Sphingomonas melonis TY
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.5 (S)-6-hydroxynicotine + H2O + O2
-
 Sphingomonas melonis 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + H2O2
-
 ?
1.5.3.5 (S)-6-hydroxynicotine + H2O + O2
-
 Sphingomonas melonis TY 1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.3.5 NdpB
-
 Sphingomonas melonis

Expression

EC Number Organism Comment Expression
1.5.3.5 Sphingomonas melonis expression is induced by nicotine or other nicotine degradation intermediates up

General Information

EC Number General Information Comment Organism
1.5.3.5 evolution enzyme is part of a nicotine-degrading gene cluster, ndp, containing the nicotine hydroxylase, 6-hydroxy-L-nicotine oxidase, 6-hydroxypseudooxynicotine oxidase, and 6-hydroxy-3-succinyl-pyridine monooxygenase responsible for catalyzing the transformation of nicotine to 2,5-dihydropyridine. The linked ndpHFEG genes shared by the variant of the pyridine and pyrrolidine pathways and pyrrolidine pathways indicate that these two pathways might share the same origin Sphingomonas melonis
1.5.3.5 physiological function after disruption of ndpB gene, the mutant strain loses the ability to grow on nicotine and accumulates 6-hydroxynicotine Sphingomonas melonis