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Literature summary extracted from

  • Wang, X.; Zhang, N.; Teng, Z.; Wang, P.; Zhang, W.; Chen, X.; Zhang, Y.; Chen, Y.; Fu, H.; Li, C.
    Structural and mechanistic insights into dimethylsulfoxide formation through dimethylsulfide oxidation (2021), Front. Microbiol., 12, 735793 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.148 structures of Tmm and Tmm soaked with dimethylsulfide. The binding of dimethylsulfide may repel the nicotinamide ring of NADP+, and make NADP+ generate a conformational change, shutting off the substrate entrance and exposing the active C4a-(hydro)peroxyflavin to dimethylsulfide to complete the oxidation Candidatus Pelagibacter sp. HTCC7211

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.148 D314A mutation decreases the activity significantly Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 D314E enzymatic activity and the apparent KM toward NADPH are only slightly affected Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 H227A increase in apparent Km value Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 N288A increase in apparent Km value Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 N72A increase in apparent Km value Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 R226A increase in apparent Km value Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 R409A increase in apparent Km value Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 S203A increase in apparent Km value Candidatus Pelagibacter sp. HTCC7211

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.148 0.0097
-
 NADPH mutant D314E, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.0128
-
 NADPH wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.032
-
 NADPH mutant D314A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.0341
-
 NADPH mutant N72A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.0412
-
 NADPH mutant H227A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.0449
-
 NADPH mutant R409A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.0611
-
 NADPH mutant N288A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.1018
-
 NADPH mutant R226A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.116
-
 Methimazole wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.1348
-
 NADPH mutant S203A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.139
-
 N,N,N-trimethylamine wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.181
-
 N,N-dimethylamine wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.25
-
 Dimethyl sulfide wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.148 Candidatus Pelagibacter sp. HTCC7211 B6BT33 dimethyl sulfoxide/trimethylamine N-oxide reductase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.148 dimethyl sulfide + NADPH + H+ + O2
-
 Candidatus Pelagibacter sp. HTCC7211 dimethyl sulfoxide + NADP+ + H2O
-
 ?
1.14.13.148 methimazole + NADPH + H+ + O2
-
 Candidatus Pelagibacter sp. HTCC7211 ? + NADP+ + H2O
-
 ?
1.14.13.148 N,N,N-trimethylamine + NADH + H+ + O2
-
 Candidatus Pelagibacter sp. HTCC7211 N,N,N-trimethylamine N-oxide + NAD+ + H2O
-
 ?
1.14.13.148 N,N-dimethylamine + NADPH + H+ + O2
-
 Candidatus Pelagibacter sp. HTCC7211 N,N-dimethylamine N-oxide + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.148 dimethyl sulfoxide/trimethylamine N-oxide reductase
-
 Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 PB7211
-
 Candidatus Pelagibacter sp. HTCC7211

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.148 0.015
-
 NADPH mutant R226A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.0167
-
 NADPH mutant S203A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.0217
-
 NADPH mutant N288A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.0317
-
 NADPH mutant N72A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.035
-
 NADPH mutant D314A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.0417
-
 NADPH mutant H227A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.045
-
 NADPH mutant R409A, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.075
-
 NADPH wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.075
-
 Dimethyl sulfide wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.0867
-
 NADPH mutant D314E, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.087
-
 Methimazole wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.298
-
 N,N-dimethylamine wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 0.373
-
 N,N,N-trimethylamine wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.148 FAD FAD and NADPH molecules are essential for the catalysis Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 NADPH FAD and NADPH molecules are essential for the catalysis Candidatus Pelagibacter sp. HTCC7211

General Information

EC Number General Information Comment Organism
1.14.13.148 metabolism the catalytic mechanism comprises a reductive half-reaction and an oxidative half-reaction. In the reductive half-reaction, FAD is reduced by NADPH. In the oxidative half-reaction, the reduced FAD reacts with O2 to form the C4a-(hydro)peroxyflavin Candidatus Pelagibacter sp. HTCC7211

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.13.148 0.3
-
 Dimethyl sulfide wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 1.64
-
 N,N-dimethylamine wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211
1.14.13.148 2.68
-
 N,N,N-trimethylamine wild-type, pH 7, 25°C Candidatus Pelagibacter sp. HTCC7211