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Literature summary extracted from

  • Weidenweber, S.; Schuehle, K.; Lippert, M.L.; Mock, J.; Seubert, A.; Demmer, U.; Ermler, U.; Heider, J.
    Finis tolueni a new type of thiolase with an integrated Zn-finger subunit catalyzes the final step of anaerobic toluene metabolism (2022), FEBS J., FEHLT, 0000 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.310 expression in Pseudomonas stutzeri Geobacter metallireducens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.310 structure of BbsAB with and without bound CoA at is determined at 1.7 A and 2.0 A resolution Geobacter metallireducens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.310 0.114
-
benzoyl-CoA calculated, kinetic model: ternary complex mechanism, pH and temperature not specified in the publication Geobacter metallireducens
2.3.1.310 0.31
-
succinyl-CoA calculated, kinetic model: ternary complex mechanism, pH and temperature not specified in the publication Geobacter metallireducens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.310 31000
-
BbsA, gel filtration Geobacter metallireducens
2.3.1.310 91000
-
BbsB, gel filtration Geobacter metallireducens
2.3.1.310 125000
-
holoenzyme, gel filtration Geobacter metallireducens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.310 (S)-2-benzoylsuccinyl-CoA + CoA Geobacter metallireducens the enzyme catalyzes the final step of anaerobic toluene metabolism benzoyl-CoA + succinyl-CoA
-
r
2.3.1.310 (S)-2-benzoylsuccinyl-CoA + CoA Geobacter metallireducens ATCC 53774 the enzyme catalyzes the final step of anaerobic toluene metabolism benzoyl-CoA + succinyl-CoA
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.310 Geobacter metallireducens Q39VG2 AND Q39VG1 Q39VG2: bbsA, Q39VG1: bbsB
-
2.3.1.310 Geobacter metallireducens ATCC 53774 Q39VG2 AND Q39VG1 Q39VG2: bbsA, Q39VG1: bbsB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.310 the BbsAB complex is purified in one step via strep-tag affinity chromatography without apparent splitting up into the respective subunits, yielding 50-60 mg pure protein per 10 g wet cell mass, further purified by size exclusion chromatography Geobacter metallireducens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.310 (S)-2-benzoylsuccinyl-CoA + CoA the enzyme catalyzes the final step of anaerobic toluene metabolism Geobacter metallireducens benzoyl-CoA + succinyl-CoA
-
r
2.3.1.310 (S)-2-benzoylsuccinyl-CoA + CoA thioester-dependent Claisen-type mechanism Geobacter metallireducens benzoyl-CoA + succinyl-CoA
-
r
2.3.1.310 (S)-2-benzoylsuccinyl-CoA + CoA the enzyme catalyzes the final step of anaerobic toluene metabolism Geobacter metallireducens ATCC 53774 benzoyl-CoA + succinyl-CoA
-
r
2.3.1.310 (S)-2-benzoylsuccinyl-CoA + CoA thioester-dependent Claisen-type mechanism Geobacter metallireducens ATCC 53774 benzoyl-CoA + succinyl-CoA
-
r
2.3.1.310 benzoyl-CoA + succinyl-CoA
-
Geobacter metallireducens (S)-2-benzoylsuccinyl-CoA + CoA
-
r
2.3.1.310 benzoyl-CoA + succinyl-CoA
-
Geobacter metallireducens ATCC 53774 (S)-2-benzoylsuccinyl-CoA + CoA
-
r

Synonyms

EC Number Synonyms Comment Organism
2.3.1.310 (S)-2-benzoylsuccinyl-CoA thiolase
-
Geobacter metallireducens
2.3.1.310 bbsAB
-
Geobacter metallireducens

General Information

EC Number General Information Comment Organism
2.3.1.310 metabolism the enzyme catalyzes the final step of anaerobic toluene metabolism Geobacter metallireducens