Literature summary extracted from
Tinikul, R.; Lawan, N.; Akeratchatapan, N.; Pimviriyakul, P.; Chinantuya, W.; Suadee, C.; Sucharitakul, J.; Chenprakhon, P.; Ballou, D.P.; Entsch, B.; Chaiyen, P.
Protonation status and control mechanism of flavin-oxygen intermediates in the reaction of bacterial luciferase (2021), FEBS J., 288, 3246-3260 .
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.14.14.3 |
H44A |
1.5% of wild-type activity |
Vibrio harveyi |
1.14.14.3 |
H44D |
2.1% of wild-type activity |
Vibrio harveyi |
1.14.14.3 |
H44N |
2.2% of wild-type activity |
Vibrio harveyi |
1.14.14.3 |
H45A |
1.7% of wild-type activity |
Vibrio harveyi |
1.14.14.3 |
H4A/H45A |
1.95% of wild-type activity |
Vibrio harveyi |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.14.3 |
Vibrio harveyi |
P07740 and P07739 |
P07740 i.e. subunit LuxA, P07739 i.e. subunit LuxB |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.14.3 |
a long-chain aldehyde + FMNH2 + O2 |
- |
Vibrio harveyi |
a long-chain fatty acid + FMN + H2O + hv |
- |
? |
|
General Information
EC Number |
General Information |
Comment |
Organism |
---|
1.14.14.3 |
metabolism |
during the reaction, the first observed flavin C4a intermediate at pH below 8.5 is the protonated flavin C4a-hydroperoxide, which loses a proton to become an active flavin C4a-peroxide. Residue His44 plays an essential role in deprotonating the flavin C4a-hydroperoxide and initiating chemical catalysis. Residue His45 has a role in binding reduced FMN |
Vibrio harveyi |