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Literature summary extracted from

  • Sasaki, D.; Watanabe, T.F.; Eady, R.R.; Garratt, R.C.; Antonyuk, S.V.; Hasnain, S.S.
    Reverse protein engineering of a novel 4-domain copper nitrite reductase reveals functional regulation by protein-protein interaction (2021), FEBS J., 288, 262-280 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.7.2.1 D439N about 7% of wild-type activity Bradyrhizobium sp. ORS 375
1.7.2.1 additional information construction of domain-truncated mutants lacking the Cyt c (18% of wild-type activity) and the Cyt c-Cup domains (3fold increase in activity) Bradyrhizobium sp. ORS 375

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.1 40000
-
 and 92000, gel filtration of mutant lacking the cyt c and cup domain Bradyrhizobium sp. ORS 375
1.7.2.1 92000
-
 and 40000, gel filtration of mutant lacking the cyt c and cup domain Bradyrhizobium sp. ORS 375
1.7.2.1 114000
-
 and 260000, gel filtration of mutant lacking the cyt c domain Bradyrhizobium sp. ORS 375
1.7.2.1 192000
-
 and 391000, gel filtration of wild-type Bradyrhizobium sp. ORS 375
1.7.2.1 260000
-
 and 114000, gel filtration of mutantlacking the cyt c domain Bradyrhizobium sp. ORS 375
1.7.2.1 391000
-
 and 192000, gel filtration of wild-type Bradyrhizobium sp. ORS 375

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.1 Bradyrhizobium sp. ORS 375 H0SHH5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.1 nitrite + reduced phenazine methosulfate
-
 Bradyrhizobium sp. ORS 375 NO + oxidized phenazine methosulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.1 BRAO375_2740002
-
 Bradyrhizobium sp. ORS 375

General Information

EC Number General Information Comment Organism
1.7.2.1 metabolism 4-domain variant of enzyme utilizes N-terminal tethering for downregulating enzymatic activity. Tethering communicates the redox state of the heme to the distant type 2 copper center that helps initiate substrate binding for catalysis Bradyrhizobium sp. ORS 375
1.7.2.1 physiological function strain has two copies of nirK, a 4-domain NiR. and a 2-domain NiR, referred to for clarity as 2DNiR. 2dNir shows anbout 5% of the activtiy of the 4-domain variant Bradyrhizobium sp. ORS 375