Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schuehle, K.; Nies, J.; Heider, J.
    An indoleacetate-CoA ligase and a phenylsuccinyl-CoA transferase involved in anaerobic metabolism of auxin (2016), Environ. Microbiol., 18, 3120-3132 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.28 expressed in Escherichia coli DH5alpha cells Aromatoleum aromaticum
6.2.1.75 expressed in Escherichia coli DH5alpha cells Aromatoleum aromaticum

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.75 (indol-3-yl)acetate substrate inhibition Aromatoleum aromaticum
6.2.1.75 2,4-dichlorophenoxyacetate
-
 Aromatoleum aromaticum
6.2.1.75 2-naphthyl acetate substrate inhibition Aromatoleum aromaticum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.3.28 0.018
-
 (R/S)-phenylsuccinyl-CoA at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.28 0.064
-
 (S)-benzylsuccinate at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.28 0.078
-
 (R/S)-benzylsuccinate at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.28 0.082
-
 (R/S)-benzylsuccinyl-CoA at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.28 0.092
-
 (R)-benzylsuccinate at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.28 0.103
-
 2-oxoindole-3-acetate at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.28 0.142
-
 indole-3-acetate at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.28 0.178
-
 (R/S)-phenylsuccinate at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
6.2.1.75 0.035
-
 (indol-3-yl)acetate pH and temperature not specified in the publication Aromatoleum aromaticum
6.2.1.75 0.097
-
 2-naphthyl acetate pH and temperature not specified in the publication Aromatoleum aromaticum
6.2.1.75 0.42
-
 phenyl acetate pH and temperature not specified in the publication Aromatoleum aromaticum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.3.28 Mg2+ 2 mM used in assay conditions Aromatoleum aromaticum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.75 ATP + (indol-3-yl)acetate + CoA Aromatoleum aromaticum
-
 AMP + diphosphate + (indol-3-yl)acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.28 Aromatoleum aromaticum
-
-
-
6.2.1.75 Aromatoleum aromaticum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.28 strep-tactin affinity column chromatography Aromatoleum aromaticum
6.2.1.75 Strep-tag affinity column chromatography Aromatoleum aromaticum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.28 0.024
-
 with (R/S)-benzylsuccinate plus (R/S)-phenylsuccinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.28 0.085
-
 with (S)-benzylsuccinate plus succinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.28 0.103
-
 with (R/S)-benzylsuccinate plus succinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.28 0.108
-
 with (R/S)-phenylsuccinate plus (R/S)-benzylsuccinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.28 0.122
-
 with (R)-benzylsuccinate plus succinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.28 0.136
-
 with 2-oxoindole-3-acetate plus succinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.28 0.188
-
 with indole-3-acetate plus succinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum
2.8.3.28 0.235
-
 with (R/S)-phenylsuccinate plus succinyl-CoA as substrates, at pH 8.0, temperature not specified in the publication Aromatoleum aromaticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.28 (R)-benzylsuccinate + succinyl-CoA 52% activity compared to (R/S)-phenylsuccinate Aromatoleum aromaticum (R)-2-benzylsuccinyl-CoA + succinate
-
 ?
2.8.3.28 (R/S)-benzylsuccinate + (R/S)-phenylsuccinyl-CoA 10% activity compared to (R/S)-phenylsuccinate plus succinyl-CoA Aromatoleum aromaticum (R/S)-benzylsuccinyl-CoA + (R/S)-phenylsuccinate
-
 ?
2.8.3.28 (R/S)-benzylsuccinate + succinyl-CoA 44% activity compared to (R/S)-phenylsuccinate Aromatoleum aromaticum (R/S)-2-benzylsuccinyl-CoA + succinate
-
 ?
2.8.3.28 (R/S)-phenylsuccinate + (R/S)-benzylsuccinyl-CoA 46% activity compared to (R/S)-phenylsuccinate plus succinyl-CoA Aromatoleum aromaticum (R/S)-phenylsuccinyl-CoA + (R/S)-benzylsuccinate
-
 ?
2.8.3.28 (R/S)-phenylsuccinate + succinyl-CoA 100% activity, (R/S)-phenylsuccinate is converted to an equal mixture of both regioisomers (2- and 3-phenylsuccinyl-CoA) Aromatoleum aromaticum (R/S)-2-phenylsuccinyl-CoA + (R/S)-3-phenylsuccinyl-CoA + succinate
-
 ?
2.8.3.28 (S)-benzylsuccinate + succinyl-CoA 36% activity compared to (R/S)-phenylsuccinate Aromatoleum aromaticum (S)-2-benzylsuccinyl-CoA + succinate
-
 ?
2.8.3.28 2'-(2-aminophenyl)succinate + succinyl-CoA
-
 Aromatoleum aromaticum 3'-(2-aminophenyl)succinyl-CoA + succinate
-
 ?
2.8.3.28 2-oxoindole-3-acetate + succinyl-CoA 58% activity compared to (R/S)-phenylsuccinate Aromatoleum aromaticum 2-oxoindole-3-acetyl-CoA + succinate
-
 ?
2.8.3.28 indole-3-acetate + succinyl-CoA 80% activity compared to (R/S)-phenylsuccinate Aromatoleum aromaticum indole-3-acetyl-CoA + succinate
-
 ?
2.8.3.28 additional information no activity with acetyl-CoA, benzylmalonate, and cinnamate Aromatoleum aromaticum ?
-
-
6.2.1.75 ATP + (indol-3-yl)acetate + CoA
-
 Aromatoleum aromaticum AMP + diphosphate + (indol-3-yl)acetyl-CoA
-
 ?
6.2.1.75 ATP + (indol-3-yl)acetate + CoA 100% activity Aromatoleum aromaticum AMP + diphosphate + (indol-3-yl)acetyl-CoA
-
 ?
6.2.1.75 ATP + 2-methyl-indole-3-acetate + CoA 6.3% activity compared to (indol-3-yl)acetate Aromatoleum aromaticum ?
-
 ?
6.2.1.75 ATP + 2-naphthyl acetate + CoA 124% activity compared to (indol-3-yl)acetate Aromatoleum aromaticum ?
-
 ?
6.2.1.75 ATP + 4-hydroxyphenyl acetate + CoA 12% activity compared to (indol-3-yl)acetate Aromatoleum aromaticum ?
-
 ?
6.2.1.75 ATP + indolepropionate + CoA 2.9% activity compared to (indol-3-yl)acetate Aromatoleum aromaticum ?
-
 ?
6.2.1.75 ATP + phenyl acetate + CoA 20% activity compared to (indol-3-yl)acetate Aromatoleum aromaticum ?
-
 ?
6.2.1.75 additional information no activity with phenyl succinate, benzyl malonate, succinate, benzyl succinate, indole-3-carboxylate, indolebutyrate or L-tryptophan Aromatoleum aromaticum ?
-
-

Subunits

EC Number Subunits Comment Organism
2.8.3.28 ? x * 40000, Strep-tagged enzyme, SDS-PAGE Aromatoleum aromaticum
2.8.3.28 ? x * 45000, Strep-tagged enzyme, calculated from amino acid sequence Aromatoleum aromaticum
6.2.1.75 monomer 1 * 55000, SDS-PAGE Aromatoleum aromaticum
6.2.1.75 monomer 1 * 57000, calculated from amino acid sequence Aromatoleum aromaticum

Synonyms

EC Number Synonyms Comment Organism
2.8.3.28 IaaL
-
 Aromatoleum aromaticum
6.2.1.75 iaaB
-
 Aromatoleum aromaticum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.75 0.48
-
 phenyl acetate pH and temperature not specified in the publication Aromatoleum aromaticum
6.2.1.75 4.3
-
 (indol-3-yl)acetate pH and temperature not specified in the publication Aromatoleum aromaticum
6.2.1.75 4.7
-
 2-naphthyl acetate pH and temperature not specified in the publication Aromatoleum aromaticum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.2.1.75 3.9
-
 (indol-3-yl)acetate pH and temperature not specified in the publication Aromatoleum aromaticum
6.2.1.75 6.5
-
 2-naphthyl acetate pH and temperature not specified in the publication Aromatoleum aromaticum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.2.1.75 1.6
-
 pH and temperature not specified in the publication Aromatoleum aromaticum 2,4-dichlorophenoxyacetate

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.75 1
-
 phenyl acetate pH and temperature not specified in the publication Aromatoleum aromaticum
6.2.1.75 48
-
 2-naphthyl acetate pH and temperature not specified in the publication Aromatoleum aromaticum
6.2.1.75 123
-
 (indol-3-yl)acetate pH and temperature not specified in the publication Aromatoleum aromaticum