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Literature summary extracted from
- Characterization of cis-4-hydroxy-D-proline dehydrogenase from Sinorhizobium meliloti (2018), Biosci. Biotechnol. Biochem., 82, 110-113 .
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.99.B4 | 0.0602 | - |
cis-4-hydroxy-D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium | Sinorhizobium meliloti |  |
| 1.4.99.B4 | 0.188 | - |
cis-4-hydroxy-D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti | |
| 1.4.99.B4 | 0.2 | - |
trans-4-hydroxy-D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium | Sinorhizobium meliloti | |
| 1.4.99.B4 | 0.401 | - |
D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium | Sinorhizobium meliloti | |
| 1.4.99.B4 | 0.473 | - |
trans-4-hydroxy-D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti | |
| 1.4.99.B4 | 0.942 | - |
D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti | |
| 1.4.99.B4 | 2 | 3 | D-methionine | pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti | |
| 1.4.99.B4 | 16.5 | - |
D-pipecolate | pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.99.B4 | cis-4-hydroxy-D-proline + acceptor | Sinorhizobium meliloti | - |
(4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate + reduced acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.99.B4 | Sinorhizobium meliloti | Q92WS2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.99.B4 | - |
Sinorhizobium meliloti |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.4.99.B4 | 0.0224 | - |
substrate: D-methionine, pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti |
| 1.4.99.B4 | 0.759 | - |
substrate: D-pipecolate, pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti |
| 1.4.99.B4 | 1.32 | - |
substrate: D-proline, pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium | Sinorhizobium meliloti |
| 1.4.99.B4 | 1.38 | - |
substrate: trans-4-hydroxy-D-proline, pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium | Sinorhizobium meliloti |
| 1.4.99.B4 | 1.4 | - |
substrate: cis-4-hydroxy-D-proline, pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium | Sinorhizobium meliloti |
| 1.4.99.B4 | 1.84 | - |
substrate: trans-4-hydroxy-D-proline, pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti |
| 1.4.99.B4 | 2 | - |
substrate: D-proline, pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti |
| 1.4.99.B4 | 3.92 | - |
substrate: cis-4-hydroxy-D-proline, pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.99.B4 | cis-4-hydroxy-D-proline + acceptor | - |
Sinorhizobium meliloti | (4R)-DELTA1-pyrroline-4-hydroxy-2-carboxylate + reduced acceptor | - |
? | |
| 1.4.99.B4 | D-methionine + acceptor + acceptor | - |
Sinorhizobium meliloti | ? | - |
? | |
| 1.4.99.B4 | D-pipecolate + acceptor + acceptor | - |
Sinorhizobium meliloti | ? | - |
? | |
| 1.4.99.B4 | D-proline + acceptor + acceptor | - |
Sinorhizobium meliloti | ? | - |
? | |
| 1.4.99.B4 | trans-4-hydroxy-D-proline + acceptor | - |
Sinorhizobium meliloti | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.4.99.B4 | SM_b20267 | - |
Sinorhizobium meliloti |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.99.B4 | 0.057 | - |
D-methionine | pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti | |
| 1.4.99.B4 | 1.02 | - |
cis-4-hydroxy-D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium | Sinorhizobium meliloti | |
| 1.4.99.B4 | 1.07 | - |
trans-4-hydroxy-D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium | Sinorhizobium meliloti | |
| 1.4.99.B4 | 1.12 | - |
D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium | Sinorhizobium meliloti | |
| 1.4.99.B4 | 1.68 | - |
trans-4-hydroxy-D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti | |
| 1.4.99.B4 | 1.92 | - |
D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti | |
| 1.4.99.B4 | 2.08 | - |
cis-4-hydroxy-D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti | |
| 1.4.99.B4 | 2.9 | - |
D-pipecolate | pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.99.B4 | 9.5 | 9.75 | - |
Sinorhizobium meliloti |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.99.B4 | FAD | FAD-containing enzyme | Sinorhizobium meliloti | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.99.B4 | 0.18 | - |
D-pipecolate | pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti | |
| 1.4.99.B4 | 2.03 | - |
D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti | |
| 1.4.99.B4 | 2.47 | - |
D-methionine | pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti | |
| 1.4.99.B4 | 2.82 | - |
D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium | Sinorhizobium meliloti | |
| 1.4.99.B4 | 3.65 | - |
trans-4-hydroxy-D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti | |
| 1.4.99.B4 | 5.37 | - |
trans-4-hydroxy-D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium | Sinorhizobium meliloti | |
| 1.4.99.B4 | 11.06 | - |
cis-4-hydroxy-D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/p-iodonitrotetrazolium violet | Sinorhizobium meliloti | |
| 1.4.99.B4 | 16.9 | - |
cis-4-hydroxy-D-proline | pH 9.0, 30°C, acceptor: phenazine methosulfate/nitroblue tetrazolium | Sinorhizobium meliloti | |
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