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Literature summary extracted from

  • Miyazaki, R.; Yamazaki, T.; Yoshimatsu, K.; Kojima, K.; Asano, R.; Sode, K.; Tsugawa, W.
    Elucidation of the intra- and inter-molecular electron transfer pathways of glucoside 3-dehydrogenase (2018), Bioelectrochemistry, 122, 115-122 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.11 expression in Escherichia coli Agrobacterium fabrum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.11 0.0034
-
 cytochrome c pH 7, 23°C Agrobacterium fabrum
1.1.2.11 3.2
-
 methyl alpha-D-glucoside pH 7, 23°C Agrobacterium fabrum

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.11 Agrobacterium fabrum A9CGJ1 i.e. catalytic subunit I, Gtd1
-
1.1.2.11 Agrobacterium fabrum C58 A9CGJ1 i.e. catalytic subunit I, Gtd1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.11 recombinant catalytic subunit I and G3DH hitch-hiker subunit II, purified by Ni2+ affinity chromatography followed by anion exchange chromatography Agrobacterium fabrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.11 methyl alpha-D-glucoside + cytochrome c
-
 Agrobacterium fabrum methyl alpha-D-3-oxoglucoside + reduced cytochrome c
-
 ?
1.1.2.11 methyl alpha-D-glucoside + cytochrome c
-
 Agrobacterium fabrum C58 methyl alpha-D-3-oxoglucoside + reduced cytochrome c
-
 ?
1.1.2.11 methyl alpha-D-glucoside + phenazine methosulfate
-
 Agrobacterium fabrum methyl alpha-D-3-oxoglucoside + reduced phenazine methosulfate
-
 ?
1.1.2.11 methyl alpha-D-glucoside + phenazine methosulfate
-
 Agrobacterium fabrum C58 methyl alpha-D-3-oxoglucoside + reduced phenazine methosulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.2.11 ? x * 63350, catalytic subunit I, calculated from sequence Agrobacterium fabrum
1.1.2.11 More enzyme is composed of the G3DH catalytic subunit I, G3DH hitch-hiker subunit II, and cytochrome c like molecule CYTc Agrobacterium fabrum

Synonyms

EC Number Synonyms Comment Organism
1.1.2.11 Atu4377
-
 Agrobacterium fabrum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.11 FAD
-
 Agrobacterium fabrum
1.1.2.11 [3Fe-4S]-center the catalytic subunit contains an 3Fe-4S iron-sulfur cluster that acts as an electron acceptor for electrons from FAD in the oxidative half reaction Agrobacterium fabrum

General Information

EC Number General Information Comment Organism
1.1.2.11 metabolism In the catalytic subunit, the reductive half reaction occurs using FAD as the electron acceptor, and the 3Fe-4S iron sulfur cluster then accepts the electron from reduced FAD. The reduced iron-sulfur cluster transfers the electron to the external cytochrome c-like molecule Agrobacterium fabrum