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Literature summary extracted from

  • Subbotina, N.M.; Chernykh, A.M.; Taranov, A.I.; Shebanova, A.D.; Moiseeva, O.V.; Ferraroni, M.; Kolomytseva, M.P.
    Gentisate 1,2-dioxygenase from the gram-positive bacteria Rhodococcus opacus 1CP Identical active sites vs. different substrate selectivities (2021), Biochimie, 180, 90-103 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.13.11.4 analysis high potential for use in biosensor technologies for monitoring the metabolism of tyrosine and salicylic acid, as well as a pollution of environment and different materials by polyaromatic compounds Rhodococcus opacus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.4 expressed in Escherichia coli strain BL21 (DE3) Rhodococcus opacus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.4 2,3-Dihydroxybenzoate
-
 Rhodococcus opacus
1.13.11.4 2,4-Dihydroxybenzoate
-
 Rhodococcus opacus
1.13.11.4 2,6-dihydroxybenzoate
-
 Rhodococcus opacus
1.13.11.4 4-Chlorocatechol
-
 Rhodococcus opacus
1.13.11.4 4-methylcatechol
-
 Rhodococcus opacus
1.13.11.4 5-chlorosalicylate
-
 Rhodococcus opacus
1.13.11.4 catechol
-
 Rhodococcus opacus
1.13.11.4 homogentisate
-
 Rhodococcus opacus
1.13.11.4 Hydroxyquinol
-
 Rhodococcus opacus
1.13.11.4 salicylate
-
 Rhodococcus opacus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.4 0.04
-
 2,5-Dihydroxybenzoate pH 8.5, 25°C Rhodococcus opacus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.4 39600
-
 calculated from sequence Rhodococcus opacus
1.13.11.4 177000
-
 gel filtration Rhodococcus opacus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.4 2,5-dihydroxybenzoate + O2 Rhodococcus opacus
-
 maleylpyruvate
-
 ?
1.13.11.4 2,5-dihydroxybenzoate + O2 Rhodococcus opacus 1CP
-
 maleylpyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.4 Rhodococcus opacus W5U174
-
-
1.13.11.4 Rhodococcus opacus 1CP W5U174
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.4
-
 Rhodococcus opacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.4 2,5-dihydroxybenzoate + O2
-
 Rhodococcus opacus maleylpyruvate
-
 ?
1.13.11.4 2,5-dihydroxybenzoate + O2 narrow substrate specificity Rhodococcus opacus maleylpyruvate
-
 ?
1.13.11.4 2,5-dihydroxybenzoate + O2
-
 Rhodococcus opacus 1CP maleylpyruvate
-
 ?
1.13.11.4 2,5-dihydroxybenzoate + O2 narrow substrate specificity Rhodococcus opacus 1CP maleylpyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.4 tetramer 4 * 41000, SDS-PAGE Rhodococcus opacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.4 40 45
-
 Rhodococcus opacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.4 7.6 8 in 50 mM Tris/H2SO4 buffer Rhodococcus opacus
1.13.11.4 8
-
 in 50 mM Clark and Lubs phosphate buffer Rhodococcus opacus
1.13.11.4 8 8.3 in 50 mM Tris/HCl buffer Rhodococcus opacus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.4 0.00145
-
 4-Chlorocatechol pH 8.5, 25°C Rhodococcus opacus
1.13.11.4 0.0067
-
 4-methylcatechol pH 8.5, 25°C Rhodococcus opacus
1.13.11.4 0.0449
-
 catechol pH 8.5, 25°C Rhodococcus opacus
1.13.11.4 0.057
-
 5-chlorosalicylate pH 8.5, 25°C Rhodococcus opacus
1.13.11.4 0.22
-
 2,6-dihydroxybenzoate pH 8.5, 25°C Rhodococcus opacus
1.13.11.4 0.33
-
 Hydroxyquinol pH 8.5, 25°C Rhodococcus opacus
1.13.11.4 0.444
-
 homogentisate pH 8.5, 25°C Rhodococcus opacus
1.13.11.4 0.81
-
 salicylate pH 8.5, 25°C Rhodococcus opacus
1.13.11.4 1.069
-
 2,3-Dihydroxybenzoate pH 8.5, 25°C Rhodococcus opacus
1.13.11.4 1.13
-
 2,4-Dihydroxybenzoate pH 8.5, 25°C Rhodococcus opacus