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Literature summary extracted from

  • van Bergen, B.; Strasser, R.; Cyr, N.; Sheppard, J.D.; Jardim, A.
    alpha,beta-Dicarbonyl reduction by Saccharomyces D-arabinose dehydrogenase (2006), Biochim. Biophys. Acta, 1760, 1636-1645 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.427 expressed in Escherichia coli ER2566 cells Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.427 0.014
-
 NADPH at pH 7.0 and 25°C Saccharomyces cerevisiae
1.1.1.427 4.2
-
 2,3-Pentanedione at pH 7.0 and 25°C Saccharomyces cerevisiae
1.1.1.427 7.7
-
 diacetyl at pH 7.0 and 25°C Saccharomyces cerevisiae
1.1.1.427 14.3
-
 methylglyoxal at pH 7.0 and 25°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.427 cytosol
-
 Saccharomyces cerevisiae 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.427 75000 80000 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.427 Saccharomyces cerevisiae
-
-
-
1.1.1.427 Saccharomyces cerevisiae BY4742
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.427 Ni-NTA column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.427 2,3-pentanedione + NADPH + H+
-
 Saccharomyces cerevisiae hydroxypentanone + NADP+
-
 ?
1.1.1.427 2,3-pentanedione + NADPH + H+
-
 Saccharomyces cerevisiae BY4742 hydroxypentanone + NADP+
-
 ?
1.1.1.427 acetoin + NADP+ this back reaction only occurs at alkaline pH values (9.0-10.0) Saccharomyces cerevisiae diacetyl + NADPH + H+
-
 r
1.1.1.427 acetoin + NADP+ this back reaction only occurs at alkaline pH values (9.0-10.0) Saccharomyces cerevisiae BY4742 diacetyl + NADPH + H+
-
 r
1.1.1.427 diacetyl + NADPH + H+
-
 Saccharomyces cerevisiae acetoin + NADP+
-
 r
1.1.1.427 diacetyl + NADPH + H+
-
 Saccharomyces cerevisiae BY4742 acetoin + NADP+
-
 r
1.1.1.427 methylglyoxal + NADPH + H+
-
 Saccharomyces cerevisiae ?
-
 ?
1.1.1.427 methylglyoxal + NADPH + H+
-
 Saccharomyces cerevisiae BY4742 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.427 homodimer 2 * 40000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.427 ARA1
-
 Saccharomyces cerevisiae
1.1.1.427 Ara1p
-
 Saccharomyces cerevisiae
1.1.1.427 D-arabinose dehydrogenase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.427 3.7
-
 NADPH at pH 7.0 and 25°C Saccharomyces cerevisiae
1.1.1.427 4.4
-
 methylglyoxal at pH 7.0 and 25°C Saccharomyces cerevisiae
1.1.1.427 5.9
-
 2,3-Pentanedione at pH 7.0 and 25°C Saccharomyces cerevisiae
1.1.1.427 6.9
-
 diacetyl at pH 7.0 and 25°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.427 4.5 5 pH optimum for the diacetyl reduction reaction Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.427 NADPH the enzyme specifically utilizes NADPH Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
1.1.1.427 malfunction enzyme knockout mutants exhibit a decreased growth rate phenotype in media containing diacetyl Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.427 0.3
-
 methylglyoxal at pH 7.0 and 25°C Saccharomyces cerevisiae
1.1.1.427 0.9
-
 diacetyl at pH 7.0 and 25°C Saccharomyces cerevisiae
1.1.1.427 1.41
-
 2,3-Pentanedione at pH 7.0 and 25°C Saccharomyces cerevisiae
1.1.1.427 257
-
 NADPH at pH 7.0 and 25°C Saccharomyces cerevisiae