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Literature summary extracted from

  • Cote, J.; Hecht, C.; Patel, K.; Ramirez-Mondragon, C.; Sham, Y.; Taylor, E.
    Opposites Attract Escherichia coli heptosyltransferase i conformational changes induced by interactions between the substrate and positively charged residues (2020), Biochemistry, 59, 3135-3147 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.99.23 K192A decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] Escherichia coli
2.4.99.23 K64A decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] Escherichia coli
2.4.99.23 K64A/R63A decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] Escherichia coli
2.4.99.23 K64A/R63A/R61A decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A]. Complete loss in blue shift, as well as an increase in the Apo lambda(max) Escherichia coli
2.4.99.23 K64A/R63A/R61A/R60A mutant enzyme with an 18-fold increase in KM, most likely because the mutations lead to structural changes in the alpha 3-N-5 loop thus impairing formation of the Michaelis complex. Complete loss in blue shift, as well as an increase in the Apo lambda(max) Escherichia coli
2.4.99.23 K98A decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherschia coli Kdo2-lipid A] Escherichia coli
2.4.99.23 R120A decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] Escherichia coli
2.4.99.23 R120A/K64A decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A]. Mutant enzyme displays an increase in protein unfolding Escherichia coli
2.4.99.23 R120A/R60A decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A]. Mutant enzyme displays an increase in protein unfolding Escherichia coli
2.4.99.23 R120A/R61A decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A]. Mutant enzyme displays an increase in protein unfolding Escherichia coli
2.4.99.23 R120A/R63A decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A]. Mutant enzyme displays an increase in protein unfolding Escherichia coli
2.4.99.23 R189A decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] Escherichia coli
2.4.99.23 R60A decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] Escherichia coli
2.4.99.23 R60A/R63A/R64A decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] Escherichia coli
2.4.99.23 R61A decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] Escherichia coli
2.4.99.23 R63A decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.99.23 0.0014
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R120A/R60A Escherichia coli
2.4.99.23 0.0025
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R61A Escherichia coli
2.4.99.23 0.0029
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, wild-type enzyme Escherichia coli
2.4.99.23 0.0029
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R120A/R61A Escherichia coli
2.4.99.23 0.006
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R189A Escherichia coli
2.4.99.23 0.006
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R60A Escherichia coli
2.4.99.23 0.008
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K64A Escherichia coli
2.4.99.23 0.01
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R63A Escherichia coli
2.4.99.23 0.011
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R120A/R63A Escherichia coli
2.4.99.23 0.014
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K192A Escherichia coli
2.4.99.23 0.015
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R120A Escherichia coli
2.4.99.23 0.016
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K98A Escherichia coli
2.4.99.23 0.02
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K64A/R63A/R61A Escherichia coli
2.4.99.23 0.034
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R120A/K64A Escherichia coli
2.4.99.23 0.037
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K64A/R63A/R61/R60A Escherichia coli
2.4.99.23 0.04
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K64A/R63A Escherichia coli
2.4.99.23 0.056
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R60A/R63A/R64A Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.99.23 Escherichia coli P24173
-
-
2.4.99.23 Escherichia coli K12 P24173
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.99.23
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.99.23 ADP-L-glycero-beta-D-manno-heptose + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A]
-
Escherichia coli ADP + alpha-Hep-(1->5)-[alpha-Kdo-(2->4)]-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A]
-
?
2.4.99.23 ADP-L-glycero-beta-D-manno-heptose + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A]
-
Escherichia coli K12 ADP + alpha-Hep-(1->5)-[alpha-Kdo-(2->4)]-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A]
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.99.23 HepI
-
Escherichia coli
2.4.99.23 heptosyltransferase I
-
Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.99.23 32
-
Tm-value, mutant apo-enzyme R60A Escherichia coli
2.4.99.23 35
-
Tm-value, mutant apo-enzyme R120A/R60A Escherichia coli
2.4.99.23 35.4
-
Tm-value, mutant apo-enzyme R63A Escherichia coli
2.4.99.23 35.7
-
Tm-value, mutant apo-enzyme K64A Escherichia coli
2.4.99.23 36.1
-
Tm-value, mutant apo-enzyme K64A/R63A/R60A Escherichia coli
2.4.99.23 36.4
-
Tm-value, mutant apo-enzyme R120A Escherichia coli
2.4.99.23 37
-
Tm-value, mutant apo-enzyme K192A Escherichia coli
2.4.99.23 38
-
Tm-value, mutant apo-enzyme R189A Escherichia coli
2.4.99.23 38
-
Tm-value, wild-type apo-enzyme Escherichia coli
2.4.99.23 38.4
-
Tm-value, mutant apo-enzyme R61A Escherichia coli
2.4.99.23 38.6
-
Tm-value, mutant apo-enzyme K64A/R63A/R61A Escherichia coli
2.4.99.23 40
-
Tm-value, mutant apo-enzyme R120A/R63A Escherichia coli
2.4.99.23 40.5
-
Tm-value, mutant apo-enzyme R120A/R61A Escherichia coli
2.4.99.23 41
-
Tm-value, mutant apo-enzyme R120A/K64A Escherichia coli
2.4.99.23 42
-
Tm-value, mutant apo-enzyme K64A/R63A/R61A/R60A Escherichia coli
2.4.99.23 43.1
-
Tm-value, mutant apo-enzyme K64A/R63A Escherichia coli
2.4.99.23 45.8
-
Tm-value, mutant apo-enzyme K98A Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.99.23 0.116
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R189A Escherichia coli
2.4.99.23 0.14
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K192A Escherichia coli
2.4.99.23 0.198
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R60A Escherichia coli
2.4.99.23 0.2
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K64A/R63A/R61A Escherichia coli
2.4.99.23 0.21
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K64A/R63A/R61A/R60A Escherichia coli
2.4.99.23 0.21
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K98A Escherichia coli
2.4.99.23 0.25
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K64A/R63A Escherichia coli
2.4.99.23 0.26
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R120A/R61A Escherichia coli
2.4.99.23 0.28
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R60A/R63A/R64A Escherichia coli
2.4.99.23 0.281
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R120A/R60A Escherichia coli
2.4.99.23 0.32
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R120A Escherichia coli
2.4.99.23 0.33
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K64A Escherichia coli
2.4.99.23 0.36
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, wild-type enzyme Escherichia coli
2.4.99.23 0.36
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R61A Escherichia coli
2.4.99.23 0.37
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R120A/R63A Escherichia coli
2.4.99.23 0.41
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R63A Escherichia coli
2.4.99.23 0.47
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R120A/K64A Escherichia coli

General Information

EC Number General Information Comment Organism
2.4.99.23 drug target disruption of the enzyme (HepI) increases susceptibility of bacteria to hydrophobic antibiotics, making HepI a potential target for drug development Escherichia coli
2.4.99.23 evolution redundancy of residues mediating conformational transitions in HepI illustrates the evolutionary importance of these structural rearrangements for catalysis Escherichia coli
2.4.99.23 malfunction disruption of the enzyme (HepI) increases susceptibility of bacteria to hydrophobic antibiotics Escherichia coli
2.4.99.23 physiological function essential enzyme for the biosynthesis of the lipopolysaccharide Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.99.23 5
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R60A/R63A/R64A Escherichia coli
2.4.99.23 6
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K64A/R63A/R61A/R60A Escherichia coli
2.4.99.23 7
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K64A/R63A Escherichia coli
2.4.99.23 10
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K64A/R63A/R61A Escherichia coli
2.4.99.23 11
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K192A Escherichia coli
2.4.99.23 13.1
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K98A Escherichia coli
2.4.99.23 14
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R120A/K64A Escherichia coli
2.4.99.23 19
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R189A Escherichia coli
2.4.99.23 21
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R120A Escherichia coli
2.4.99.23 30
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R120A/R63A Escherichia coli
2.4.99.23 33
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R60A Escherichia coli
2.4.99.23 41
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme K64A Escherichia coli
2.4.99.23 41
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R63A Escherichia coli
2.4.99.23 90
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R120A/R61A Escherichia coli
2.4.99.23 124
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, wild-type enzyme Escherichia coli
2.4.99.23 140
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R61A Escherichia coli
2.4.99.23 200
-
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] pH 7.5, 37°C, mutant enzyme R120A/R60A Escherichia coli