EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.99.23 | K192A | decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | Escherichia coli |
2.4.99.23 | K64A | decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | Escherichia coli |
2.4.99.23 | K64A/R63A | decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | Escherichia coli |
2.4.99.23 | K64A/R63A/R61A | decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A]. Complete loss in blue shift, as well as an increase in the Apo lambda(max) | Escherichia coli |
2.4.99.23 | K64A/R63A/R61A/R60A | mutant enzyme with an 18-fold increase in KM, most likely because the mutations lead to structural changes in the alpha 3-N-5 loop thus impairing formation of the Michaelis complex. Complete loss in blue shift, as well as an increase in the Apo lambda(max) | Escherichia coli |
2.4.99.23 | K98A | decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherschia coli Kdo2-lipid A] | Escherichia coli |
2.4.99.23 | R120A | decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | Escherichia coli |
2.4.99.23 | R120A/K64A | decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A]. Mutant enzyme displays an increase in protein unfolding | Escherichia coli |
2.4.99.23 | R120A/R60A | decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A]. Mutant enzyme displays an increase in protein unfolding | Escherichia coli |
2.4.99.23 | R120A/R61A | decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A]. Mutant enzyme displays an increase in protein unfolding | Escherichia coli |
2.4.99.23 | R120A/R63A | decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A]. Mutant enzyme displays an increase in protein unfolding | Escherichia coli |
2.4.99.23 | R189A | decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | Escherichia coli |
2.4.99.23 | R60A | decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | Escherichia coli |
2.4.99.23 | R60A/R63A/R64A | decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | Escherichia coli |
2.4.99.23 | R61A | decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | Escherichia coli |
2.4.99.23 | R63A | decrease in kcat/Km for alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.99.23 | 0.0014 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R120A/R60A | Escherichia coli | |
2.4.99.23 | 0.0025 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R61A | Escherichia coli | |
2.4.99.23 | 0.0029 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, wild-type enzyme | Escherichia coli | |
2.4.99.23 | 0.0029 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R120A/R61A | Escherichia coli | |
2.4.99.23 | 0.006 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R189A | Escherichia coli | |
2.4.99.23 | 0.006 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R60A | Escherichia coli | |
2.4.99.23 | 0.008 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K64A | Escherichia coli | |
2.4.99.23 | 0.01 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R63A | Escherichia coli | |
2.4.99.23 | 0.011 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R120A/R63A | Escherichia coli | |
2.4.99.23 | 0.014 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K192A | Escherichia coli | |
2.4.99.23 | 0.015 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R120A | Escherichia coli | |
2.4.99.23 | 0.016 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K98A | Escherichia coli | |
2.4.99.23 | 0.02 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K64A/R63A/R61A | Escherichia coli | |
2.4.99.23 | 0.034 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R120A/K64A | Escherichia coli | |
2.4.99.23 | 0.037 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K64A/R63A/R61/R60A | Escherichia coli | |
2.4.99.23 | 0.04 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K64A/R63A | Escherichia coli | |
2.4.99.23 | 0.056 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R60A/R63A/R64A | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.99.23 | Escherichia coli | P24173 | - |
- |
2.4.99.23 | Escherichia coli K12 | P24173 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.99.23 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.99.23 | ADP-L-glycero-beta-D-manno-heptose + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | - |
Escherichia coli | ADP + alpha-Hep-(1->5)-[alpha-Kdo-(2->4)]-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | - |
? | |
2.4.99.23 | ADP-L-glycero-beta-D-manno-heptose + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | - |
Escherichia coli K12 | ADP + alpha-Hep-(1->5)-[alpha-Kdo-(2->4)]-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.99.23 | HepI | - |
Escherichia coli |
2.4.99.23 | heptosyltransferase I | - |
Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.99.23 | 32 | - |
Tm-value, mutant apo-enzyme R60A | Escherichia coli |
2.4.99.23 | 35 | - |
Tm-value, mutant apo-enzyme R120A/R60A | Escherichia coli |
2.4.99.23 | 35.4 | - |
Tm-value, mutant apo-enzyme R63A | Escherichia coli |
2.4.99.23 | 35.7 | - |
Tm-value, mutant apo-enzyme K64A | Escherichia coli |
2.4.99.23 | 36.1 | - |
Tm-value, mutant apo-enzyme K64A/R63A/R60A | Escherichia coli |
2.4.99.23 | 36.4 | - |
Tm-value, mutant apo-enzyme R120A | Escherichia coli |
2.4.99.23 | 37 | - |
Tm-value, mutant apo-enzyme K192A | Escherichia coli |
2.4.99.23 | 38 | - |
Tm-value, mutant apo-enzyme R189A | Escherichia coli |
2.4.99.23 | 38 | - |
Tm-value, wild-type apo-enzyme | Escherichia coli |
2.4.99.23 | 38.4 | - |
Tm-value, mutant apo-enzyme R61A | Escherichia coli |
2.4.99.23 | 38.6 | - |
Tm-value, mutant apo-enzyme K64A/R63A/R61A | Escherichia coli |
2.4.99.23 | 40 | - |
Tm-value, mutant apo-enzyme R120A/R63A | Escherichia coli |
2.4.99.23 | 40.5 | - |
Tm-value, mutant apo-enzyme R120A/R61A | Escherichia coli |
2.4.99.23 | 41 | - |
Tm-value, mutant apo-enzyme R120A/K64A | Escherichia coli |
2.4.99.23 | 42 | - |
Tm-value, mutant apo-enzyme K64A/R63A/R61A/R60A | Escherichia coli |
2.4.99.23 | 43.1 | - |
Tm-value, mutant apo-enzyme K64A/R63A | Escherichia coli |
2.4.99.23 | 45.8 | - |
Tm-value, mutant apo-enzyme K98A | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.99.23 | 0.116 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R189A | Escherichia coli | |
2.4.99.23 | 0.14 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K192A | Escherichia coli | |
2.4.99.23 | 0.198 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R60A | Escherichia coli | |
2.4.99.23 | 0.2 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K64A/R63A/R61A | Escherichia coli | |
2.4.99.23 | 0.21 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K64A/R63A/R61A/R60A | Escherichia coli | |
2.4.99.23 | 0.21 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K98A | Escherichia coli | |
2.4.99.23 | 0.25 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K64A/R63A | Escherichia coli | |
2.4.99.23 | 0.26 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R120A/R61A | Escherichia coli | |
2.4.99.23 | 0.28 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R60A/R63A/R64A | Escherichia coli | |
2.4.99.23 | 0.281 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R120A/R60A | Escherichia coli | |
2.4.99.23 | 0.32 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R120A | Escherichia coli | |
2.4.99.23 | 0.33 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K64A | Escherichia coli | |
2.4.99.23 | 0.36 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, wild-type enzyme | Escherichia coli | |
2.4.99.23 | 0.36 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R61A | Escherichia coli | |
2.4.99.23 | 0.37 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R120A/R63A | Escherichia coli | |
2.4.99.23 | 0.41 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R63A | Escherichia coli | |
2.4.99.23 | 0.47 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R120A/K64A | Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.99.23 | drug target | disruption of the enzyme (HepI) increases susceptibility of bacteria to hydrophobic antibiotics, making HepI a potential target for drug development | Escherichia coli |
2.4.99.23 | evolution | redundancy of residues mediating conformational transitions in HepI illustrates the evolutionary importance of these structural rearrangements for catalysis | Escherichia coli |
2.4.99.23 | malfunction | disruption of the enzyme (HepI) increases susceptibility of bacteria to hydrophobic antibiotics | Escherichia coli |
2.4.99.23 | physiological function | essential enzyme for the biosynthesis of the lipopolysaccharide | Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.99.23 | 5 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R60A/R63A/R64A | Escherichia coli | |
2.4.99.23 | 6 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K64A/R63A/R61A/R60A | Escherichia coli | |
2.4.99.23 | 7 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K64A/R63A | Escherichia coli | |
2.4.99.23 | 10 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K64A/R63A/R61A | Escherichia coli | |
2.4.99.23 | 11 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K192A | Escherichia coli | |
2.4.99.23 | 13.1 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K98A | Escherichia coli | |
2.4.99.23 | 14 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R120A/K64A | Escherichia coli | |
2.4.99.23 | 19 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R189A | Escherichia coli | |
2.4.99.23 | 21 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R120A | Escherichia coli | |
2.4.99.23 | 30 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R120A/R63A | Escherichia coli | |
2.4.99.23 | 33 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R60A | Escherichia coli | |
2.4.99.23 | 41 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme K64A | Escherichia coli | |
2.4.99.23 | 41 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R63A | Escherichia coli | |
2.4.99.23 | 90 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R120A/R61A | Escherichia coli | |
2.4.99.23 | 124 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, wild-type enzyme | Escherichia coli | |
2.4.99.23 | 140 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R61A | Escherichia coli | |
2.4.99.23 | 200 | - |
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[O-deacylated Escherichia coli Kdo2-lipid A] | pH 7.5, 37°C, mutant enzyme R120A/R60A | Escherichia coli |