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Literature summary extracted from

  • Eschenbrenner, M.; Chlumsky, L.J.; Khanna, P.; Strasser, F.; Jorns, M.S.
    Organization of the multiple coenzymes and subunits and role of the covalent flavin link in the complex heterotetrameric sarcosine oxidase (2001), Biochemistry, 40, 5352-5367 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.24 expressed in Escherichia coli BL21(DE3) cells Corynebacterium sp. P-1

Protein Variants

EC Number Protein Variants Comment Organism
1.5.3.24 C141S the mutant exhibits specific activity values reduced to those of the wild type recombinant enzyme or the natural enzyme Corynebacterium sp. P-1
1.5.3.24 C195S the mutant exhibits specific activity values similar to those of the wild type recombinant enzyme or the natural enzyme Corynebacterium sp. P-1
1.5.3.24 C351S the mutant exhibits specific activity values similar to those of the wild type recombinant enzyme or the natural enzyme Corynebacterium sp. P-1
1.5.3.24 G139A the mutant of the alpha subunit is expressed only at low temperature (15°C), but the purified enzyme exhibits properties indistinguishable from the wild type enzyme Corynebacterium sp. P-1
1.5.3.24 G30A the mutant of the beta subunit exhibits subunit expression levels similar to those of the wild type enzyme, but the mutation blocks subunit assembly and covalent attachment of FMN Corynebacterium sp. P-1
1.5.3.24 H173N catalytically inactive mutant of the beta subunit Corynebacterium sp. P-1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.24 sarcosine + 5,6,7,8-tetrahydrofolate + O2 Corynebacterium sp. P-1
-
 glycine + 5,10-methylenetetrahydrofolate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.24 Corynebacterium sp. P-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.24 phenyl-Sepharose column chromatography and Talon affinity resin column chromatography Corynebacterium sp. P-1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.24 methylthioacetate + 5,6,7,8-tetrahydrofolate + O2
-
 Corynebacterium sp. P-1 ?
-
 ?
1.5.3.24 sarcosine + 5,6,7,8-tetrahydrofolate + O2
-
 Corynebacterium sp. P-1 glycine + 5,10-methylenetetrahydrofolate + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.24 heterotetramer
-
 Corynebacterium sp. P-1

Synonyms

EC Number Synonyms Comment Organism
1.5.3.24 heterotetrameric sarcosine oxidase
-
 Corynebacterium sp. P-1
1.5.3.24 TSOX
-
 Corynebacterium sp. P-1

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.24 FAD
-
 Corynebacterium sp. P-1
1.5.3.24 FMN
-
 Corynebacterium sp. P-1
1.5.3.24 NAD+
-
 Corynebacterium sp. P-1