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Literature summary extracted from

  • Huang, C.W.; Hwang, C.C.; Chang, Y.L.; Liu, J.T.; Wu, S.P.; Huang, K.L.; Huang, W.M.; Lee, H.J.
    Functional role of residues involved in substrate binding of human 4-hydroxyphenylpyruvate dioxygenase (2021), Biochem. J., 478, 2201-2215 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.27 comparison of Arabidopsis thaiana and human enzyme. In human, the carboxyl group of substrate 4-hydroxyphenylpyruvate interacts by a H-bond network formed by Gln334, metal-binding ligand Glu349, and Asn363 in the C-terminal helix. The 4-hydroxyl group of the substrate interacts with Gln251 and Gln265 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.27 N363A about 20% of wild-type activity Homo sapiens
1.13.11.27 N363D about 6% of wild-type activity Homo sapiens
1.13.11.27 N363Q about 7% of wild-type activity Homo sapiens
1.13.11.27 Q251E almost complete loss of activity Homo sapiens
1.13.11.27 Q265E about 5% of wild-type activity Homo sapiens
1.13.11.27 Q334A relative activity and substrate-binding affinity are preserved Homo sapiens
1.13.11.27 Q334A/N363D about 5% of wild-type activity Homo sapiens
1.13.11.27 Q334A/N363Q about 3% of wild-type activity Homo sapiens
1.13.11.27 Q334N about 3% of wild-type activity Homo sapiens
1.13.11.27 Q334N/N363Q about 2% of wild-type activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.27 0.2
-
 4-hydroxyphenylpyruvate wild-type, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.2
-
 4-hydroxyphenylpyruvate mutant Q334N, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.3
-
 4-hydroxyphenylpyruvate mutant N363A, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.3
-
 4-hydroxyphenylpyruvate mutant Q334A, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.4
-
 4-hydroxyphenylpyruvate mutant N363Q, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.5
-
 4-hydroxyphenylpyruvate mutant N363D, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.5
-
 4-hydroxyphenylpyruvate mutant Q334A/N363D, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.5
-
 4-hydroxyphenylpyruvate mutant Q334N/N363Q, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.6
-
 4-hydroxyphenylpyruvate mutant Q334A/N363Q, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.9
-
 4-hydroxyphenylpyruvate mutant Q265E, pH 6.5, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.27 Homo sapiens P32754
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2
-
 Homo sapiens homogentisate + CO2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.27 52.6
-
 melting temperature, wild-type Homo sapiens
1.13.11.27 54.3
-
 melting temperature, wild-type in presence of Fe2+ Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.27 0.06
-
 4-hydroxyphenylpyruvate mutant Q334N, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.09
-
 4-hydroxyphenylpyruvate mutant Q334N/N363Q, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.1
-
 4-hydroxyphenylpyruvate mutant Q265E, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.2
-
 4-hydroxyphenylpyruvate mutant Q334A/N363D, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.2
-
 4-hydroxyphenylpyruvate mutant Q334A/N363Q, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.3
-
 4-hydroxyphenylpyruvate mutant N363D, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.3
-
 4-hydroxyphenylpyruvate mutant N363Q, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.6
-
 4-hydroxyphenylpyruvate mutant N363A, pH 6.5, 37°C Homo sapiens
1.13.11.27 1
-
 4-hydroxyphenylpyruvate mutant Q334A, pH 6.5, 37°C Homo sapiens
1.13.11.27 2
-
 4-hydroxyphenylpyruvate wild-type, pH 6.5, 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.27 0.2
-
 4-hydroxyphenylpyruvate mutant Q265E, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.2
-
 4-hydroxyphenylpyruvate mutant Q334N/N363Q, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.3
-
 4-hydroxyphenylpyruvate mutant Q334A/N363Q, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.3
-
 4-hydroxyphenylpyruvate mutant Q334N, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.5
-
 4-hydroxyphenylpyruvate mutant Q334A/N363D, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.6
-
 4-hydroxyphenylpyruvate mutant N363D, pH 6.5, 37°C Homo sapiens
1.13.11.27 0.7
-
 4-hydroxyphenylpyruvate mutant N363Q, pH 6.5, 37°C Homo sapiens
1.13.11.27 2
-
 4-hydroxyphenylpyruvate mutant N363A, pH 6.5, 37°C Homo sapiens
1.13.11.27 4
-
 4-hydroxyphenylpyruvate mutant Q334A, pH 6.5, 37°C Homo sapiens
1.13.11.27 10
-
 4-hydroxyphenylpyruvate wild-type, pH 6.5, 37°C Homo sapiens