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Literature summary extracted from

  • Seo, D.; Choi, K.
    Heterologous production of pyomelanin biopolymer using 4-hydroxyphenylpyruvate dioxygenase isolated from Ralstonia pickettii in Escherichia coli (2020), Biochem. Eng. J., 157, 107548.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.27 expression in Escherichia coli Ralstonia pickettii

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.27 synthesis recombinant Escherichia coli expression HppD can produce pyomelanin at levels up to 315.5 mg/l (13.1 mg/l/h) in 24 h. Highest pyomelanin production is achieved when L-tyrosine and 0.5mM Cu2+ are supplemented into LB medium Ralstonia pickettii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.27 soluble
-
 Ralstonia pickettii
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.27 Ralstonia pickettii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2
-
 Ralstonia pickettii homogentisate + CO2
-
 ?