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Literature summary extracted from

  • Zhang, H.; Zhang, X.; Geng, A.
    Expression of a novel manganese peroxidase from Cerrena unicolor BBP6 in Pichia pastoris and its application in dye decolorization and PAH degradation (2020), Biochem. Eng. J., 153, 107402 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.11.1.13 environmental protection as to denim bleaching, sodium hypochlorite treatment is primarily used and this gives rise to problems such as chemical injuries, denim yellowness and reduced denim strength. To ensure the low-cost and ecofriendly advantages, denim biobleaching using oxidizing enzymes such as manganese peroxidases (MnPs) and laccases is an ideal alternative. In the presence of MnPs, denim bleaching by laccases is greatly enhanced. Usage of recombinant white-rot fungi MnP in denim bleaching and PAH degradation Cerrena unicolor
1.11.1.13 additional information the recombinant isotzyme MnP3 from Cerrena unicolor strain BBP6, rMnP3-BBP6, has promising biotechnological application potential in textile industries and polycyclic aromatic hydrocarbon bioremediation Cerrena unicolor

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.13 gene mnp3, recombinant expression of isozyme MnP3 as soluble extracellular protein in Pichia pastoris using alpha-factor as the signal peptide the recombinant enzyme has a MnP activity of 154.5 U/l Cerrena unicolor

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.13 extracellular manganese peroxidase (MnP) is an extracellular glycosylated heme protein Cerrena unicolor
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.13 Ca2+ two Ca2+ per subunit Cerrena unicolor
1.11.1.13 Fe2+ in the heme b group Cerrena unicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.13 2 Mn(II) + 2 H+ + H2O2 Cerrena unicolor
-
2 Mn(III) + 2 H2O
-
?
1.11.1.13 2 Mn(II) + 2 H+ + H2O2 Cerrena unicolor BBP6
-
2 Mn(III) + 2 H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.13 Cerrena unicolor A0A7D5FUQ6
-
-
1.11.1.13 Cerrena unicolor BBP6 A0A7D5FUQ6
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.13 glycoprotein manganese peroxidase (MnP) is an extracellular glycosylated heme protein Cerrena unicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.13 2 Mn(II) + 2 H+ + H2O2
-
Cerrena unicolor 2 Mn(III) + 2 H2O
-
?
1.11.1.13 2 Mn(II) + 2 H+ + H2O2
-
Cerrena unicolor BBP6 2 Mn(III) + 2 H2O
-
?
1.11.1.13 brilliant blue R + H2O2 dye decolorization Cerrena unicolor ?
-
?
1.11.1.13 bromophenol blue + H2O2 dye decolorization Cerrena unicolor ?
-
?
1.11.1.13 bromophenol blue + H2O2 dye decolorization Cerrena unicolor BBP6 ?
-
?
1.11.1.13 crystal violet + H2O2 dye decolorization Cerrena unicolor ?
-
?
1.11.1.13 crystal violet + H2O2 dye decolorization Cerrena unicolor BBP6 ?
-
?
1.11.1.13 fluorene + H2O2 denim bleaching PAH degradation, product analysis by HPLC Cerrena unicolor 9H-fluorene-3,4-diol
-
?
1.11.1.13 methyl orange + H2O2 dye decolorization Cerrena unicolor ?
-
?
1.11.1.13 additional information isozyme MnP3 shows a broad substrate specificity Cerrena unicolor ?
-
-
1.11.1.13 additional information isozyme MnP3 shows a broad substrate specificity Cerrena unicolor BBP6 ?
-
-
1.11.1.13 phenanthrene + 2 H+ + 2 H2O2 denim bleaching PAH degradation, product analysis by HPLC Cerrena unicolor phenanthrene-9,10-dione + 2 H2O
-
?
1.11.1.13 Remazol Brilliant Blue R + H2O2 dye decolorization Cerrena unicolor ?
-
?
1.11.1.13 Remazol Brilliant Blue R + H2O2 dye decolorization Cerrena unicolor BBP6 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.13 MnP3
-
Cerrena unicolor
1.11.1.13 rMnP3-BBP6
-
Cerrena unicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.13 55
-
-
Cerrena unicolor

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.11.1.13 20 70 activity range, profile overview Cerrena unicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.13 20 55 over 80% of maximal activity remains, recombinant enzyme, temperature stability profile, overview Cerrena unicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.13 4.7
-
-
Cerrena unicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.13 3.5 6 activity range, profile overview Cerrena unicolor

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.13 3.5 4.7 over 50% of maximal activity remains, recombinant enzyme, pH stability profile, overview Cerrena unicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.13 heme b manganese peroxidase (MnP) is an extracellular glycosylated heme protein, it harbors one heme b (iron(II)-protoporphyrin IX) group per subunit Cerrena unicolor

General Information

EC Number General Information Comment Organism
1.11.1.13 physiological function manganese peroxidase (MnP) is an extracellular glycosylated heme protein produced by various basidiomycetous fungi. It requires hydrogen peroxide as an oxidant to function. In the catalytic cycle of MnP, Mn2+ is oxidized into Mn3+. A complex of Mn3+ and organic acid with low molecular weight is subsequently formed and acts as a diffusible redox-mediator to attack phenolic lignin structures and break the aromatic rings of lignin polymers. The recombinant isozyme MnP3 from Cerrena unicolor strain BBP6 has strong decolorizing activity on a variety of dyes and it is efficient in denim bleaching. It is also able to degrade fluorene and phenanthrene effectively. Due to its broad substrate specificity, MnP is capable of transforming many structurally different pollutants such as synthetic dyes, PAHs and pesticides Cerrena unicolor