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Literature summary extracted from

  • Wang, B.; Zhang, Z.; Zhu, H.; Niu, C.; Wen, X.; Xi, Z.
    The hydrogen bonding network involved Arg59 in human protoporphyrinogen IX oxidase is essential for enzyme activity (2021), Biochem. Biophys. Res. Commun., 557, 20-25 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.4 expressed in Escherichia coli BL21(DE3)pLysS cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.3.3.4 D349A the mutant enzyme maintains 8.05% of wild type activity Homo sapiens
1.3.3.4 D349E the mutant enzyme maintains 84.78% of wild type activity Homo sapiens
1.3.3.4 D349R the mutant enzyme maintains 2.48% of wild type activity Homo sapiens
1.3.3.4 R59A the mutant enzyme maintains 44.13% of wild type activity Homo sapiens
1.3.3.4 R59E the mutant enzyme maintains 22.72% of wild type activity Homo sapiens
1.3.3.4 R59K the mutant enzyme maintains 82.46% of wild type activity Homo sapiens
1.3.3.4 R59W the mutation dramatically decreases the activity of the enzyme Homo sapiens
1.3.3.4 T366A the mutant enzyme maintains 65.45% of wild type activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.4 0.001162
-
 protoporphyrinogen IX mutant enzyme R59A, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 0.001253
-
 protoporphyrinogen IX mutant enzyme D349E, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 0.001405
-
 protoporphyrinogen IX wild type enzyme, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 0.001768
-
 protoporphyrinogen IX mutant enzyme T366A, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 0.001865
-
 protoporphyrinogen IX mutant enzyme R59K, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 0.00208
-
 protoporphyrinogen IX mutant enzyme D349A, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 0.00298
-
 protoporphyrinogen IX mutant enzyme R59E, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 0.00358
-
 protoporphyrinogen IX mutant enzyme D349R, at pH 7.4 and 25°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.4 protoporphyrinogen IX + 3 O2 Homo sapiens
-
 protoporphyrin IX + 3 H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.4 Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.4 protoporphyrinogen IX + 3 O2
-
 Homo sapiens protoporphyrin IX + 3 H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.4 PPO
-
 Homo sapiens
1.3.3.4 protoporphyrinogen IX oxidase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.3.4 0.012
-
 protoporphyrinogen IX mutant enzyme D349R, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 0.023
-
 protoporphyrinogen IX mutant enzyme D349A, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 0.07
-
 protoporphyrinogen IX mutant enzyme R59A, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 0.092
-
 protoporphyrinogen IX mutant enzyme R59E, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 0.145
-
 protoporphyrinogen IX mutant enzyme D349E, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 0.155
-
 protoporphyrinogen IX mutant enzyme T366A, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 0.192
-
 protoporphyrinogen IX wild type enzyme, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 0.21
-
 protoporphyrinogen IX mutant enzyme R59K, at pH 7.4 and 25°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.3.4 FAD
-
 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.3.4 3.4
-
 protoporphyrinogen IX mutant enzyme D349R, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 11
-
 protoporphyrinogen IX mutant enzyme D349A, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 31
-
 protoporphyrinogen IX mutant enzyme R59E, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 60.2
-
 protoporphyrinogen IX mutant enzyme R59A, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 87.9
-
 protoporphyrinogen IX mutant enzyme T366A, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 112.5
-
 protoporphyrinogen IX mutant enzyme R59K, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 115.7
-
 protoporphyrinogen IX mutant enzyme D349E, at pH 7.4 and 25°C Homo sapiens
1.3.3.4 136.4
-
 protoporphyrinogen IX wild type enzyme, at pH 7.4 and 25°C Homo sapiens