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Literature summary extracted from
- Peculiarities of nitronate monooxygenases and perspectives for in vivo and in vitro applications (2021), Appl. Microbiol. Biotechnol., 105, 8019-8032.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.12.16 | 0.34 | - |
propionate-3-nitronate | pH and temperature not specified in the publication | Neurospora crassa |  |
| 1.13.12.16 | 0.58 | - |
3-nitropropionate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 1.4 | - |
hexyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 3.1 | - |
ethyl nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 5 | - |
propyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 10 | - |
butyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 24 | - |
1-Nitropropane | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 29 | - |
nitroethane | pH and temperature not specified in the publication | Neurospora crassa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.7.3.1 | 185000 | - |
gel filtration | Fusarium oxysporum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.7.3.1 | Fusarium oxysporum | - |
- |
- |
| 1.13.12.16 | Neurospora crassa | Q01284 | - |
- |
| 1.13.12.16 | Neurospora crassa DSM 1257 | Q01284 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.7.3.1 | 1-nitropropane + H2O + O2 | - |
Fusarium oxysporum | propionaldehyde + nitrite + H2O2 | - |
? | |
| 1.7.3.1 | additional information | the enzyme does not oxidize acyl-CoA substrates and prefers primary nitroalkanes containing four or more carbon atoms | Fusarium oxysporum | ? | - |
? | |
| 1.7.3.1 | nitroethane + H2O | - |
Fusarium oxysporum | acetaldehyde + nitrite + H2O2 | - |
? | |
| 1.13.12.16 | 1-nitropropane + O2 | - |
Neurospora crassa | ? | - |
? | |
| 1.13.12.16 | 1-nitropropane + O2 | - |
Neurospora crassa DSM 1257 | ? | - |
? | |
| 1.13.12.16 | 3-nitropropionate + O2 | - |
Neurospora crassa | ? | - |
? | |
| 1.13.12.16 | 3-nitropropionate + O2 | - |
Neurospora crassa DSM 1257 | ? | - |
? | |
| 1.13.12.16 | butyl 1-nitronate + O2 | - |
Neurospora crassa | ? | - |
? | |
| 1.13.12.16 | ethyl nitronate + O2 | - |
Neurospora crassa | ? | - |
? | |
| 1.13.12.16 | hexyl 1-nitronate + O2 | - |
Neurospora crassa | ? | - |
? | |
| 1.13.12.16 | additional information | the enzyme can catalyze the oxidation of neutral or anionic nitroalkanes and alkyl nitronates to their corresponding carbonyl and nitro compounds | Neurospora crassa | ? | - |
? | |
| 1.13.12.16 | additional information | the enzyme can catalyze the oxidation of neutral or anionic nitroalkanes and alkyl nitronates to their corresponding carbonyl and nitro compounds | Neurospora crassa DSM 1257 | ? | - |
? | |
| 1.13.12.16 | nitroethane + O2 | - |
Neurospora crassa | ? | - |
? | |
| 1.13.12.16 | nitroethane + O2 | - |
Neurospora crassa DSM 1257 | ? | - |
? | |
| 1.13.12.16 | pentyl 1-nitronate + O2 | - |
Neurospora crassa | ? | - |
? | |
| 1.13.12.16 | propionate-3-nitronate + O2 | - |
Neurospora crassa | ? | - |
? | |
| 1.13.12.16 | propionate-3-nitronate + O2 | - |
Neurospora crassa DSM 1257 | ? | - |
? | |
| 1.13.12.16 | propyl 1-nitronate + O2 | - |
Neurospora crassa | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.7.3.1 | homodimer or homotetramer | - |
Fusarium oxysporum |
| 1.13.12.16 | homodimer | - |
Neurospora crassa |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.7.3.1 | NAO | - |
Fusarium oxysporum |
| 1.13.12.16 | ncd2 | - |
Neurospora crassa |
| 1.13.12.16 | NMO | - |
Neurospora crassa |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.12.16 | 4.4 | - |
3-nitropropionate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 14 | - |
nitroethane | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 20 | - |
1-Nitropropane | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 22 | - |
hexyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 51 | - |
ethyl nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 55 | - |
butyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 60 | - |
propyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 430 | - |
propionate-3-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.3.1 | FAD | - |
Fusarium oxysporum | |
| 1.13.12.16 | FMN | - |
Neurospora crassa | |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.7.3.1 | Fusarium oxysporum | the enzyme expression is induced in media containing nitroethane | up |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.13.12.16 | 0.83 | - |
1-Nitropropane | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 1.2 | - |
pentyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 5.6 | - |
butyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 7.6 | - |
3-nitropropionate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 11.4 | - |
propyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 16 | - |
hexyl 1-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 16.5 | - |
ethyl nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
| 1.13.12.16 | 1300 | - |
propionate-3-nitronate | pH and temperature not specified in the publication | Neurospora crassa | |
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