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Literature summary extracted from

  • Cervantes Quintero, K.Y.; Padilla Guerrero, I.E.; Torres Guzman, J.C.; Villa Martinez, B.G.; Valencia Felix, A.; Gonzalez Hernandez, G.A.
    Members of the nitronate monooxygenase gene family from Metarhizium brunneum are induced during the process of infection to Plutella xylostella (2020), Appl. Microbiol. Biotechnol., 104, 2987-2997 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.16 expressed in Escherichia coli BL21(DE3) pLysS cells Metarhizium brunneum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.16 0.45
-
 1-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 1.3
-
 1-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 1.6
-
 2-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 2.8
-
 2-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 3.4
-
 nitroethane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 4.32
-
 2-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 5.6
-
 2-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 10.6
-
 nitroethane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 12.9
-
 1-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 13.6
-
 1-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 20.1
-
 nitroethane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 34.3
-
 1-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 45.1
-
 nitroethane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 50.6
-
 nitroethane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 91.4
-
 1-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 134.9
-
 2-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 147.8
-
 nitroethane at pH 6.5 and 28°C Metarhizium brunneum
1.13.12.16 148.5
-
 2-Nitropropane at pH 6.5 and 28°C Metarhizium brunneum

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.16 Metarhizium brunneum A0A6C0WWH2
-
-
1.13.12.16 Metarhizium brunneum A0A6C0WVH0
-
-
1.13.12.16 Metarhizium brunneum A0A6C0WVK2
-
-
1.13.12.16 Metarhizium brunneum A0A6C0WW14
-
-
1.13.12.16 Metarhizium brunneum
-
-
-
1.13.12.16 Metarhizium brunneum Ma10 A0A6C0WWH2
-
-
1.13.12.16 Metarhizium brunneum Ma10 A0A6C0WVH0
-
-
1.13.12.16 Metarhizium brunneum Ma10 A0A6C0WVK2
-
-
1.13.12.16 Metarhizium brunneum Ma10 A0A6C0WW14
-
-
1.13.12.16 Metarhizium brunneum Ma10
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.16 HisTrap column chromatography Metarhizium brunneum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.12.16 mycelium
-
 Metarhizium brunneum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.16 1-nitropropane + O2
-
 Metarhizium brunneum propionaldehyde + HNO2
-
 ?
1.13.12.16 1-nitropropane + O2
-
 Metarhizium brunneum Ma10 propionaldehyde + HNO2
-
 ?
1.13.12.16 2-nitropropane + O2
-
 Metarhizium brunneum acetone + HNO2
-
 ?
1.13.12.16 2-nitropropane + O2
-
 Metarhizium brunneum Ma10 acetone + HNO2
-
 ?
1.13.12.16 nitroethane + O2
-
 Metarhizium brunneum ?
-
 ?
1.13.12.16 nitroethane + O2
-
 Metarhizium brunneum Ma10 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.16 NMO
-
 Metarhizium brunneum
1.13.12.16 Npd1
-
 Metarhizium brunneum
1.13.12.16 Npd2
-
 Metarhizium brunneum
1.13.12.16 Npd3
-
 Metarhizium brunneum
1.13.12.16 Npd4
-
 Metarhizium brunneum
1.13.12.16 Npd5
-
 Metarhizium brunneum
1.13.12.16 Npd6
-
 Metarhizium brunneum

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.16 FMN
-
 Metarhizium brunneum

Expression

EC Number Organism Comment Expression
1.13.12.16 Metarhizium brunneum the enzyme is expressed during Metarhizium brunneum infection of Plutella xylostella up