EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.251 | expression in Escherichia coli | Chromohalobacter salexigens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.251 | 0.0439 | - |
NADH | cosubstrate FAD, pH 7,25°C | Chromohalobacter salexigens | |
1.14.13.251 | 0.258 | - |
NADPH | cosubstrate FAD, pH 7,25°C | Chromohalobacter salexigens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.251 | Iron | contents of iron and acid-labile sulfide in the purified enzyme are 1.88 mol and 1.76 mol per mol BmoB | Chromohalobacter salexigens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.251 | 45100 | - |
subunit BmoB, gel filtration | Chromohalobacter salexigens |
1.14.13.251 | 154800 | - |
subunit BmoA, gel filtration | Chromohalobacter salexigens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.251 | glycine betaine + NADH + H+ + O2 | Chromohalobacter salexigens | - |
N,N-dimethylglycine + formaldehyde + NAD+ + H2O | - |
? | |
1.14.13.251 | glycine betaine + NADH + H+ + O2 | Chromohalobacter salexigens DSM 3043 | - |
N,N-dimethylglycine + formaldehyde + NAD+ + H2O | - |
? | |
1.14.13.251 | glycine betaine + NADPH + H+ + O2 | Chromohalobacter salexigens | - |
N,N-dimethylglycine + formaldehyde + NADP+ + H2O | - |
? | |
1.14.13.251 | glycine betaine + NADPH + H+ + O2 | Chromohalobacter salexigens DSM 3043 | - |
N,N-dimethylglycine + formaldehyde + NADP+ + H2O | - |
? | |
1.14.13.251 | additional information | Chromohalobacter salexigens | FMN is the most favored electron acceptor for BmoB compared with FAD and riboflavin | ? | - |
- |
|
1.14.13.251 | additional information | Chromohalobacter salexigens DSM 3043 | FMN is the most favored electron acceptor for BmoB compared with FAD and riboflavin | ? | - |
- |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.251 | Chromohalobacter salexigens | Q1QYU6 and Q1QYU7 | Q1QYU6 i.e. oxidoreductase FAD-binding protein BmoB, Q1QYU7 i.e. Rieske (2Fe-2S) protein BmoA | - |
1.14.13.251 | Chromohalobacter salexigens DSM 3043 | Q1QYU6 and Q1QYU7 | Q1QYU6 i.e. oxidoreductase FAD-binding protein BmoB, Q1QYU7 i.e. Rieske (2Fe-2S) protein BmoA | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.13.251 | BmoA-His6 solution is very unstable and maintains no more than 10% residual activity when stored for 2 weeks at -40°C in elution buffer with no addition of enzyme stabilizer | Chromohalobacter salexigens |
1.14.13.251 | His6-BmoB is stable for at least 2 weeks when stored at -40°C in elution buffer with no addition of enzyme stabilizer | Chromohalobacter salexigens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.251 | glycine betaine + NADH + H+ + O2 | - |
Chromohalobacter salexigens | N,N-dimethylglycine + formaldehyde + NAD+ + H2O | - |
? | |
1.14.13.251 | glycine betaine + NADH + H+ + O2 | - |
Chromohalobacter salexigens DSM 3043 | N,N-dimethylglycine + formaldehyde + NAD+ + H2O | - |
? | |
1.14.13.251 | glycine betaine + NADPH + H+ + O2 | - |
Chromohalobacter salexigens | N,N-dimethylglycine + formaldehyde + NADP+ + H2O | - |
? | |
1.14.13.251 | glycine betaine + NADPH + H+ + O2 | - |
Chromohalobacter salexigens DSM 3043 | N,N-dimethylglycine + formaldehyde + NADP+ + H2O | - |
? | |
1.14.13.251 | additional information | FMN is the most favored electron acceptor for BmoB compared with FAD and riboflavin | Chromohalobacter salexigens | ? | - |
- |
|
1.14.13.251 | additional information | FMN is the most favored electron acceptor for BmoB compared with FAD and riboflavin | Chromohalobacter salexigens DSM 3043 | ? | - |
- |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.251 | tetramer | 3 * 52500, subunit BmoA, plus 1 * 47700, subunit BmoB, SDS-PAGE | Chromohalobacter salexigens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.251 | BmoA | - |
Chromohalobacter salexigens |
1.14.13.251 | BmoB | - |
Chromohalobacter salexigens |
1.14.13.251 | Csal_1004 | - |
Chromohalobacter salexigens |
1.14.13.251 | Csal_1005 | - |
Chromohalobacter salexigens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.251 | 0.35 | - |
NADPH | cosubstrate FAD, pH 7,25°C | Chromohalobacter salexigens | |
1.14.13.251 | 0.9 | - |
NADH | cosubstrate FAD, pH 7,25°C | Chromohalobacter salexigens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.251 | FAD | each BmoB monomer contains one FAD molecule | Chromohalobacter salexigens | |
1.14.13.251 | NAD+ | - |
Chromohalobacter salexigens | |
1.14.13.251 | NADH | NADH is the preferred electron donor over NADPH | Chromohalobacter salexigens | |
1.14.13.251 | NADPH | NADH is the preferred electron donor over NADPH | Chromohalobacter salexigens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.13.251 | evolution | BmoA belongs to group V of the Rieske nonheme iron oxygenase family | Chromohalobacter salexigens |
1.14.13.251 | metabolism | in the presence of BmoB, NADH, and flavin, BmoA can aerobically degrade glycine betaine to dimethylglycine with the concomitant production of formaldehyde. BmoA exhibits strict substrate specificity for glycine betaine, and its demethylation activity is stimulated by Fe2+ | Chromohalobacter salexigens |
1.14.13.251 | physiological function | enzyme is involved in a degradation pathway of glycine betaine. Escherichia coli expressing Bmoa/BmoB accumulates dimethylglycine. BmoB is an NADH-dependent flavin reductase with one noncovalently bound flavin adenine dinucleotide (FAD) as its prosthetic group | Chromohalobacter salexigens |