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Literature summary extracted from

  • Shao, Y.; Guo, L.; Zhang, Y.; Yu, H.; Zhao, B.; Pang, H.; Lu, W.
    Glycine betaine monooxygenase, an unusual Riesketype oxygenase system, catalyzes the oxidative N-demethylation of glycine betaine in Chromohalobacter salexigens DSM 3043 (2018), Appl. Environ. Microbiol., 84, e00377-18 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.251 expression in Escherichia coli Chromohalobacter salexigens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.251 0.0439
-
NADH cosubstrate FAD, pH 7,25°C Chromohalobacter salexigens
1.14.13.251 0.258
-
NADPH cosubstrate FAD, pH 7,25°C Chromohalobacter salexigens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.251 Iron contents of iron and acid-labile sulfide in the purified enzyme are 1.88 mol and 1.76 mol per mol BmoB Chromohalobacter salexigens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.251 45100
-
subunit BmoB, gel filtration Chromohalobacter salexigens
1.14.13.251 154800
-
subunit BmoA, gel filtration Chromohalobacter salexigens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.251 glycine betaine + NADH + H+ + O2 Chromohalobacter salexigens
-
N,N-dimethylglycine + formaldehyde + NAD+ + H2O
-
?
1.14.13.251 glycine betaine + NADH + H+ + O2 Chromohalobacter salexigens DSM 3043
-
N,N-dimethylglycine + formaldehyde + NAD+ + H2O
-
?
1.14.13.251 glycine betaine + NADPH + H+ + O2 Chromohalobacter salexigens
-
N,N-dimethylglycine + formaldehyde + NADP+ + H2O
-
?
1.14.13.251 glycine betaine + NADPH + H+ + O2 Chromohalobacter salexigens DSM 3043
-
N,N-dimethylglycine + formaldehyde + NADP+ + H2O
-
?
1.14.13.251 additional information Chromohalobacter salexigens FMN is the most favored electron acceptor for BmoB compared with FAD and riboflavin ?
-
-
1.14.13.251 additional information Chromohalobacter salexigens DSM 3043 FMN is the most favored electron acceptor for BmoB compared with FAD and riboflavin ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.251 Chromohalobacter salexigens Q1QYU6 and Q1QYU7 Q1QYU6 i.e. oxidoreductase FAD-binding protein BmoB, Q1QYU7 i.e. Rieske (2Fe-2S) protein BmoA
-
1.14.13.251 Chromohalobacter salexigens DSM 3043 Q1QYU6 and Q1QYU7 Q1QYU6 i.e. oxidoreductase FAD-binding protein BmoB, Q1QYU7 i.e. Rieske (2Fe-2S) protein BmoA
-

Storage Stability

EC Number Storage Stability Organism
1.14.13.251 BmoA-His6 solution is very unstable and maintains no more than 10% residual activity when stored for 2 weeks at -40°C in elution buffer with no addition of enzyme stabilizer Chromohalobacter salexigens
1.14.13.251 His6-BmoB is stable for at least 2 weeks when stored at -40°C in elution buffer with no addition of enzyme stabilizer Chromohalobacter salexigens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.251 glycine betaine + NADH + H+ + O2
-
Chromohalobacter salexigens N,N-dimethylglycine + formaldehyde + NAD+ + H2O
-
?
1.14.13.251 glycine betaine + NADH + H+ + O2
-
Chromohalobacter salexigens DSM 3043 N,N-dimethylglycine + formaldehyde + NAD+ + H2O
-
?
1.14.13.251 glycine betaine + NADPH + H+ + O2
-
Chromohalobacter salexigens N,N-dimethylglycine + formaldehyde + NADP+ + H2O
-
?
1.14.13.251 glycine betaine + NADPH + H+ + O2
-
Chromohalobacter salexigens DSM 3043 N,N-dimethylglycine + formaldehyde + NADP+ + H2O
-
?
1.14.13.251 additional information FMN is the most favored electron acceptor for BmoB compared with FAD and riboflavin Chromohalobacter salexigens ?
-
-
1.14.13.251 additional information FMN is the most favored electron acceptor for BmoB compared with FAD and riboflavin Chromohalobacter salexigens DSM 3043 ?
-
-

Subunits

EC Number Subunits Comment Organism
1.14.13.251 tetramer 3 * 52500, subunit BmoA, plus 1 * 47700, subunit BmoB, SDS-PAGE Chromohalobacter salexigens

Synonyms

EC Number Synonyms Comment Organism
1.14.13.251 BmoA
-
Chromohalobacter salexigens
1.14.13.251 BmoB
-
Chromohalobacter salexigens
1.14.13.251 Csal_1004
-
Chromohalobacter salexigens
1.14.13.251 Csal_1005
-
Chromohalobacter salexigens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.251 0.35
-
NADPH cosubstrate FAD, pH 7,25°C Chromohalobacter salexigens
1.14.13.251 0.9
-
NADH cosubstrate FAD, pH 7,25°C Chromohalobacter salexigens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.251 FAD each BmoB monomer contains one FAD molecule Chromohalobacter salexigens
1.14.13.251 NAD+
-
Chromohalobacter salexigens
1.14.13.251 NADH NADH is the preferred electron donor over NADPH Chromohalobacter salexigens
1.14.13.251 NADPH NADH is the preferred electron donor over NADPH Chromohalobacter salexigens

General Information

EC Number General Information Comment Organism
1.14.13.251 evolution BmoA belongs to group V of the Rieske nonheme iron oxygenase family Chromohalobacter salexigens
1.14.13.251 metabolism in the presence of BmoB, NADH, and flavin, BmoA can aerobically degrade glycine betaine to dimethylglycine with the concomitant production of formaldehyde. BmoA exhibits strict substrate specificity for glycine betaine, and its demethylation activity is stimulated by Fe2+ Chromohalobacter salexigens
1.14.13.251 physiological function enzyme is involved in a degradation pathway of glycine betaine. Escherichia coli expressing Bmoa/BmoB accumulates dimethylglycine. BmoB is an NADH-dependent flavin reductase with one noncovalently bound flavin adenine dinucleotide (FAD) as its prosthetic group Chromohalobacter salexigens