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Literature summary extracted from

  • Li, K.; Fielding, E.N.; Condurso, H.L.; Bruner, S.D.
    Probing the structural basis of oxygen binding in a cofactor-independent dioxygenase (2017), Acta Crystallogr. Sect. D, 73, 573-580.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.80 expressed in Escherichia coli BL21(DE3) cells Streptomyces toyocaensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.80 wild type and mutant enzymes A319C and V425T in complex with substrate, hanging drop vapor diffusion method, using 140 mM ammonium acetate, 14% (w/v) PEG 4000, 100 mM sodium citrate pH 5.6 Streptomyces toyocaensis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.80 A319C the catalytic efficiency of the mutant is about 1.5% of that of the wild type protein Streptomyces toyocaensis
1.13.11.80 V425T the mutant shows reduced activity compared to the wild type enzyme Streptomyces toyocaensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.80 dihydroxyphenylacetyl-amine-CoA
-
 Streptomyces toyocaensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.80 0.125
-
 (3,5-dihydroxyphenyl)acetyl-CoA mutant enzyme A319C, at pH 7.5 and 24°C Streptomyces toyocaensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.80 (3,5-dihydroxyphenyl)acetyl-CoA + O2 Streptomyces toyocaensis
-
 2-(3,5-dihydroxyphenyl)-2-oxoacetate + CoA
-
 ?
1.13.11.80 (3,5-dihydroxyphenyl)acetyl-CoA + O2 Streptomyces toyocaensis A47934
-
 2-(3,5-dihydroxyphenyl)-2-oxoacetate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.80 Streptomyces toyocaensis Q8KLK7
-
-
1.13.11.80 Streptomyces toyocaensis A47934 Q8KLK7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.80 Ni-NTA column chromatography and Superdex 200 gel filtration Streptomyces toyocaensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.80 (3,5-dihydroxyphenyl)acetyl-CoA + O2
-
 Streptomyces toyocaensis 2-(3,5-dihydroxyphenyl)-2-oxoacetate + CoA
-
 ?
1.13.11.80 (3,5-dihydroxyphenyl)acetyl-CoA + O2
-
 Streptomyces toyocaensis A47934 2-(3,5-dihydroxyphenyl)-2-oxoacetate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.80 ? x * 48000, SDS-PAGE Streptomyces toyocaensis

Synonyms

EC Number Synonyms Comment Organism
1.13.11.80 DpgC
-
 Streptomyces toyocaensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.80 0.095
-
 (3,5-dihydroxyphenyl)acetyl-CoA mutant enzyme A319C, at pH 7.5 and 24°C Streptomyces toyocaensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.80 additional information the enzyme is cofactor-independent Streptomyces toyocaensis