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Literature summary extracted from

  • Romero, E.; Savino, S.; Fraaije, M.W.; Loncar, N.
    Mechanistic and crystallographic studies of azoreductase AzoA from Bacillus wakoensis A01 (2020), ACS Chem. Biol., 15, 504-512 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.7.1.17 in complex with FMN, sitting drop vapor diffusion method, using 0.17 M ammonium sulfate and 25.2% (w/v) PEG 4000 Alkalihalobacillus wakoensis

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.17 W60D the mutant retains more than 10% of wild type activity Alkalihalobacillus wakoensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.17 0.025
-
 menadione mutant enzyme W60A, at pH 8.5 and 25°C Alkalihalobacillus wakoensis
1.7.1.17 0.061
-
 1,2-naphthoquinone wild type enzyme, at pH 8.5 and 25°C Alkalihalobacillus wakoensis
1.7.1.17 0.062
-
 1,2-naphthoquinone mutant enzyme W60A, at pH 8.5 and 25°C Alkalihalobacillus wakoensis
1.7.1.17 0.064
-
 1,4-Naphthoquinone mutant enzyme W60A, at pH 8.5 and 25°C Alkalihalobacillus wakoensis
1.7.1.17 0.069
-
 menadione wild type enzyme, at pH 8.5 and 25°C Alkalihalobacillus wakoensis
1.7.1.17 0.097
-
 1,4-Naphthoquinone wild type enzyme, at pH 8.5 and 25°C Alkalihalobacillus wakoensis
1.7.1.17 0.115
-
 Orange I wild type enzyme, at pH 8.5 and 25°C Alkalihalobacillus wakoensis
1.7.1.17 0.157
-
 Orange I mutant enzyme W60A, at pH 8.5 and 25°C Alkalihalobacillus wakoensis

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.17 Alkalihalobacillus wakoensis A0A455X8R2
-
-
1.7.1.17 Alkalihalobacillus wakoensis A01 A0A455X8R2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.17 Ni-Sepharose column chromatography Alkalihalobacillus wakoensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.17 1,2-naphthoquinone + 2 NADH + 2 H+
-
 Alkalihalobacillus wakoensis ?
-
 ?
1.7.1.17 1,2-naphthoquinone + 2 NADH + 2 H+
-
 Alkalihalobacillus wakoensis A01 ?
-
 ?
1.7.1.17 1,4-naphthoquinone + 2 NADH + 2 H+
-
 Alkalihalobacillus wakoensis ?
-
 ?
1.7.1.17 1,4-naphthoquinone + 2 NADH + 2 H+
-
 Alkalihalobacillus wakoensis A01 ?
-
 ?
1.7.1.17 menadione + 2 NADH + 2 H+
-
 Alkalihalobacillus wakoensis ?
-
 ?
1.7.1.17 menadione + 2 NADH + 2 H+
-
 Alkalihalobacillus wakoensis A01 ?
-
 ?
1.7.1.17 additional information the enzyme shows a negligible ability to use molecular oxygen as an electron acceptor Alkalihalobacillus wakoensis ?
-
-
1.7.1.17 additional information the enzyme shows a negligible ability to use molecular oxygen as an electron acceptor Alkalihalobacillus wakoensis A01 ?
-
-
1.7.1.17 Orange I + 2 NADH + 2 H+
-
 Alkalihalobacillus wakoensis ?
-
 ?
1.7.1.17 Orange I + 2 NADH + 2 H+
-
 Alkalihalobacillus wakoensis A01 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.17 homodimer
-
 Alkalihalobacillus wakoensis

Synonyms

EC Number Synonyms Comment Organism
1.7.1.17 AzoA
-
 Alkalihalobacillus wakoensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.17 71
-
 apparent melting temperature Alkalihalobacillus wakoensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.1.17 0.85
-
 Orange I wild type enzyme, at pH 8.5 and 25°C Alkalihalobacillus wakoensis
1.7.1.17 1.06
-
 Orange I mutant enzyme W60A, at pH 8.5 and 25°C Alkalihalobacillus wakoensis
1.7.1.17 67
-
 1,2-naphthoquinone mutant enzyme W60A, at pH 8.5 and 25°C Alkalihalobacillus wakoensis
1.7.1.17 111
-
 1,2-naphthoquinone wild type enzyme, at pH 8.5 and 25°C Alkalihalobacillus wakoensis
1.7.1.17 136
-
 menadione mutant enzyme W60A, at pH 8.5 and 25°C Alkalihalobacillus wakoensis
1.7.1.17 152
-
 1,4-Naphthoquinone mutant enzyme W60A, at pH 8.5 and 25°C Alkalihalobacillus wakoensis
1.7.1.17 340
-
 1,4-Naphthoquinone wild type enzyme, at pH 8.5 and 25°C Alkalihalobacillus wakoensis
1.7.1.17 387
-
 menadione wild type enzyme, at pH 8.5 and 25°C Alkalihalobacillus wakoensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.17 FMN
-
 Alkalihalobacillus wakoensis
1.7.1.17 NADH the enzyme is strictly NADH-dependent Alkalihalobacillus wakoensis