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Literature summary extracted from

  • Wang, B.; Luo, L.; Wang, D.; Ding, R.; Hong, J.
    Efficient purification of a recombinant tag-free thermostable Kluyveromyces marxianus uricase by pH-induced self-cleavage of intein and expression in Escherichia coli (2018), 3 Biotech, 8, 400 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.3.3 acetone 108.42% activity at 20% (v/v) Kluyveromyces marxianus
1.7.3.3 DMSO 103.19% activity at 20% (v/v) Kluyveromyces marxianus
1.7.3.3 EDTA 110.57% activity at 20% (v/v) Kluyveromyces marxianus
1.7.3.3 n-hexane 102.21% activity at 20% (v/v) Kluyveromyces marxianus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.3.3 expressed in Escherichia coli BL21(DE3) cells Kluyveromyces marxianus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.3 2-mercaptoethanol 62.98% residual activity at 20% (v/v) Kluyveromyces marxianus
1.7.3.3 acetaldehyde complete inhibition at 20% (v/v) Kluyveromyces marxianus
1.7.3.3 Ca2+ 95.32% residual activity at 1 mM Kluyveromyces marxianus
1.7.3.3 Cu2+ 19.82% residual activity at 1 mM Kluyveromyces marxianus
1.7.3.3 dithiothreitol 94.93% residual activity at 20% (v/v) Kluyveromyces marxianus
1.7.3.3 ethanol 71.97% residual activity at 20% (v/v) Kluyveromyces marxianus
1.7.3.3 Fe2+ 6.07% residual activity at 1 mM Kluyveromyces marxianus
1.7.3.3 Fe3+ complete inhibition at 1 mM Kluyveromyces marxianus
1.7.3.3 H2O2 68.68% residual activity at 0.02 mM Kluyveromyces marxianus
1.7.3.3 K+ 88.69% residual activity at 1 mM Kluyveromyces marxianus
1.7.3.3 methanol 84.55% residual activity at 20% (v/v) Kluyveromyces marxianus
1.7.3.3 Mg2+ 87.99% residual activity at 1 mM Kluyveromyces marxianus
1.7.3.3 Na+ 90.92% residual activity at 1 mM Kluyveromyces marxianus
1.7.3.3 Ni2+ 84.77% residual activity at 1 mM Kluyveromyces marxianus
1.7.3.3 PEG4000 68.04% residual activity at 20% (v/v) Kluyveromyces marxianus
1.7.3.3 Tween 80 95.26% residual activity at 20% (v/v) Kluyveromyces marxianus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.3.3 0.0676
-
 Urate at pH 9.0 and 42°C Kluyveromyces marxianus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.3.3 urate + O2 + H2O Kluyveromyces marxianus
-
 5-hydroxyisourate + H2O2
-
 ?
1.7.3.3 urate + O2 + H2O Kluyveromyces marxianus NBRC 1777
-
 5-hydroxyisourate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.3 Kluyveromyces marxianus
-
-
-
1.7.3.3 Kluyveromyces marxianus NBRC 1777
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.3.3 RAC Kluyveromyces marxianus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.3.3 5.36
-
 soluble cell lysate, at pH 9.0 and 42°C Kluyveromyces marxianus
1.7.3.3 50.54
-
 after 9.43fold purification, at pH 9.0 and 42°C Kluyveromyces marxianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.3 urate + O2 + H2O
-
 Kluyveromyces marxianus 5-hydroxyisourate + H2O2
-
 ?
1.7.3.3 urate + O2 + H2O
-
 Kluyveromyces marxianus NBRC 1777 5-hydroxyisourate + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.3.3 uricase
-
 Kluyveromyces marxianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.3.3 42
-
-
 Kluyveromyces marxianus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.7.3.3 40 45 the retained enzymatic activity at 40 and 45°C is 91.63% and 93.32%, respectively Kluyveromyces marxianus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.3.3 40
-
 the enzyme retains 79.75% of activity when incubated at 40°C for 90 h. After incubation of the enzyme for 15 min at 40 and 45°C, the retained activities are 95.55% and 74.55%, respectivelyThe retained activity decreases significantly when the incubation temperature is above 55°C Kluyveromyces marxianus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.3.3 32.74
-
 Urate at pH 9.0 and 42°C Kluyveromyces marxianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.3.3 9
-
-
 Kluyveromyces marxianus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.3.3 6 11 the enzyme activity remains stable after 15 min at pH 6.0-11.0 (4, 25 and 37°C) Kluyveromyces marxianus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7.3.3 484.32
-
 Urate at pH 9.0 and 42°C Kluyveromyces marxianus