Literature summary extracted from

Levin, B.J.; Huang, Y.Y.; Peck, S.C.; Wei, Y.; Martinez-Del Campo, A.; Marks, J.A.; Franzosa, E.A.; Huttenhower, C.; Balskus, E.P.
A prominent glycyl radical enzyme in human gut microbiomes metabolizes trans-4-hydroxy-L-proline (2017), Science, 355, eaai8386.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.2.1.172	expression in Escherichia coli	Clostridioides difficile

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.2.1.172	1.2	-	trans-4-hydroxy-L-proline	pH not specified in the publication, temperature not specified in the publication	Clostridioides difficile

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.1.172	Clostridioides difficile	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.2.1.172	additional information	enzyme displays undetectable or greatly reduced activity toward other hydroxyproline stereoisomers	Clostridioides difficile	?	-	-
4.2.1.172	trans-4-hydroxy-L-proline	-	Clostridioides difficile	(S)-1-pyrroline-5-carboxylate + H2O	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
4.2.1.172	t4LHypD	-	Clostridioides difficile

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.2.1.172	45	-	trans-4-hydroxy-L-proline	pH not specified in the publication, temperature not specified in the publication	Clostridioides difficile

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
4.2.1.172	38	-	trans-4-hydroxy-L-proline	pH not specified in the publication, temperature not specified in the publication	Clostridioides difficile

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Release 2023.1 (January 2023)