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Literature summary extracted from

  • Kim, T.S.; Gao, H.; Li, J.; Kalia, V.C.; Muthusamy, K.; Sohng, J.K.; Kim, I.W.; Lee, J.K.
    Overcoming NADPH product inhibition improves D-sorbitol conversion to L-sorbose (2019), Sci. Rep., 9, 815 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.B60 synthesis synthesis of L-sorbose is largely used as a starting material for L-ascorbic acid biosynthesis. It can been also used to synthesize the potent glycosidase inhibitor 1-deoxygalactonojirim and rare sugars such as L-tagatose and L-iditol. Escherichia coli(gosldh-lrenox) producing both GoSLDH for D-sorbitol oxidation and LreNOX (NAD(P)H oxidase from Lactobacillus reuteri) for NADP+ regeneration is generated and used for L-sorbose production. L-Sorbose production by Escherichi coli(gosldh-lrenox) reaches 4.1 g/l after 40 min, which was 20.5fold higher than that of Escherichia coli(gosldh). This system reduces the NADPH inhibition effect in the GoSLDH reaction and enables high production of L-sorbose from D-sorbitol Gluconobacter oxydans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B60 Escherichia coli(gosldh-lrenox) producing both GoSLDH for D-sorbitol oxidation and LreNOX (NAD(P)H oxidase from Lactobacillus reuteri) for NADP+ regeneration is generated and used for L-sorbose production. L-Sorbose production by Escherichi coli(gosldh-lrenox) reaches 4.1 g/l after 40 min, which was 20.5fold higher than that of Escherichia coli(gosldh). This system reduces the NADPH inhibition effect in the GoSLDH reaction and enables high production of L-sorbose from D-sorbitol Gluconobacter oxydans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.B60 NADPH product inhibition. Escherichia coli(gosldh-lrenox) producing both GoSLDH for D-sorbitol oxidation and LreNOX (NAD(P)H oxidase from Lactobacillus reuteri) for NADP+ regeneration is generated and used for L-sorbose production. L-Sorbose production by Escherichi coli(gosldh-lrenox) reaches 4.1 g/l after 40 min, which was 20.5fold higher than that of Escherichia coli(gosldh). This system reduces the NADPH inhibition effect in the GoSLDH reaction and enables high production of L-sorbose from D-sorbitol Gluconobacter oxydans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B60 Gluconobacter oxydans
-
-
-
1.1.1.B60 Gluconobacter oxydans G624
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.B60
-
 Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B60 D-sorbitol + NADP+ strict NADP+-dependent Gluconobacter oxydans L-sorbose + NADPH + H+
-
 ?
1.1.1.B60 D-sorbitol + NADP+ strict NADP+-dependent Gluconobacter oxydans G624 L-sorbose + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B60 GoSLDH
-
 Gluconobacter oxydans
1.1.1.B60 sorbitol dehydrogenase
-
 Gluconobacter oxydans