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Literature summary extracted from

  • Xu, J.Z.; Ruan, H.Z.; Liu, L.M.; Wang, L.P.; Zhang, W.G.
    Overexpression of thermostable meso-diaminopimelate dehydrogenase to redirect diaminopimelate pathway for increasing L-lysine production in Escherichia coli (2019), Sci. Rep., 9, 2423 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.4.1.16 synthesis expression of DapDH in Escherichia coli significantly enhances carbon flux into the pentose phosphate pathway and L-lysine biosynthetic pathway, thus increasing the levels of NADPH and precursors for L-lysine biosynthesis. The coexistence of two DAP-pathways and sufficient ammonium availability are good for increasing the final titer of L-lysine with a high carbon yield and productivity in Escherichia coli. Fed-batch fermentation of the target strain results in 119.5 g/l of L-lysine with a carbon yield of 49.1% and productivity of 2.99 g per l and h Symbiobacterium thermophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.16 0.11
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Acetivibrio thermocellus
1.4.1.16 0.11
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Bacteroides fragilis
1.4.1.16 0.21
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Acetivibrio thermocellus
1.4.1.16 0.23
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Lysinibacillus sphaericus
1.4.1.16 0.24
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Symbiobacterium thermophilum
1.4.1.16 0.26
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Ureibacillus thermosphaericus
1.4.1.16 0.27
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Corynebacterium glutamicum
1.4.1.16 0.57
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Bacteroides fragilis
1.4.1.16 1.8
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Symbiobacterium thermophilum
1.4.1.16 1.9
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Ureibacillus thermosphaericus
1.4.1.16 2.4
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Lysinibacillus sphaericus
1.4.1.16 2.8
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Corynebacterium glutamicum
1.4.1.16 4.2
-
 NH4+ pH 8.5, 30°C Bacteroides fragilis
1.4.1.16 6.3
-
 NH4+ pH 8.5, 30°C Symbiobacterium thermophilum
1.4.1.16 6.5
-
 NH4+ pH 8.5, 30°C Ureibacillus thermosphaericus
1.4.1.16 8 9 NH4+ pH 8.5, 30°C Acetivibrio thermocellus
1.4.1.16 10.8
-
 NH4+ pH 8.5, 30°C Lysinibacillus sphaericus
1.4.1.16 39.2
-
 NH4+ pH 8.5, 30°C Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.16 Acetivibrio thermocellus A3DDX7
-
-
1.4.1.16 Acetivibrio thermocellus ATCC 27405 A3DDX7
-
-
1.4.1.16 Bacteroides fragilis Q64PZ8
-
-
1.4.1.16 Bacteroides fragilis YCH46 Q64PZ8
-
-
1.4.1.16 Corynebacterium glutamicum P04964
-
-
1.4.1.16 Corynebacterium glutamicum ATCC 13032 P04964
-
-
1.4.1.16 Lysinibacillus sphaericus Q9KWR0
-
-
1.4.1.16 Symbiobacterium thermophilum Q67PI3
-
-
1.4.1.16 Ureibacillus thermosphaericus G1UII1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.16 L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Corynebacterium glutamicum meso-2,6-diaminoheptanedioate + NADP+
-
 ?
1.4.1.16 L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Symbiobacterium thermophilum meso-2,6-diaminoheptanedioate + NADP+
-
 ?
1.4.1.16 L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Ureibacillus thermosphaericus meso-2,6-diaminoheptanedioate + NADP+
-
 ?
1.4.1.16 L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Lysinibacillus sphaericus meso-2,6-diaminoheptanedioate + NADP+
-
 ?
1.4.1.16 L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Acetivibrio thermocellus meso-2,6-diaminoheptanedioate + NADP+
-
 ?
1.4.1.16 L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Bacteroides fragilis meso-2,6-diaminoheptanedioate + NADP+
-
 ?
1.4.1.16 L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Bacteroides fragilis YCH46 meso-2,6-diaminoheptanedioate + NADP+
-
 ?
1.4.1.16 L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Corynebacterium glutamicum ATCC 13032 meso-2,6-diaminoheptanedioate + NADP+
-
 ?
1.4.1.16 L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 Acetivibrio thermocellus ATCC 27405 meso-2,6-diaminoheptanedioate + NADP+
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + NADP+
-
 Corynebacterium glutamicum L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + NADP+
-
 Symbiobacterium thermophilum L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + NADP+
-
 Ureibacillus thermosphaericus L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + NADP+
-
 Lysinibacillus sphaericus L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + NADP+
-
 Acetivibrio thermocellus L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + NADP+
-
 Bacteroides fragilis L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + NADP+
-
 Bacteroides fragilis YCH46 L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + NADP+
-
 Corynebacterium glutamicum ATCC 13032 L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + NADP+
-
 Acetivibrio thermocellus ATCC 27405 L-DELTA1-tetrahydrodipicolinate + NH4+ + NADPH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.16 BAB07799
-
 Lysinibacillus sphaericus
1.4.1.16 BF3690
-
 Bacteroides fragilis
1.4.1.16 Sth1425
-
 Symbiobacterium thermophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.16 4.9
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Bacteroides fragilis
1.4.1.16 7.7
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Bacteroides fragilis
1.4.1.16 41.9
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Corynebacterium glutamicum
1.4.1.16 50.6
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Ureibacillus thermosphaericus
1.4.1.16 52.8
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Symbiobacterium thermophilum
1.4.1.16 64.7
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Lysinibacillus sphaericus
1.4.1.16 115.4
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Corynebacterium glutamicum
1.4.1.16 123.3
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Ureibacillus thermosphaericus
1.4.1.16 145.7
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Symbiobacterium thermophilum
1.4.1.16 154.2
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Lysinibacillus sphaericus
1.4.1.16 160.8
-
 NH4+ pH 8.5, 30°C Bacteroides fragilis
1.4.1.16 278.2
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Acetivibrio thermocellus
1.4.1.16 344.3
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Acetivibrio thermocellus
1.4.1.16 347.7
-
 NH4+ pH 8.5, 30°C Ureibacillus thermosphaericus
1.4.1.16 387.4
-
 NH4+ pH 8.5, 30°C Symbiobacterium thermophilum
1.4.1.16 511.9
-
 NH4+ pH 8.5, 30°C Lysinibacillus sphaericus
1.4.1.16 768.3
-
 NH4+ pH 8.5, 30°C Corynebacterium glutamicum
1.4.1.16 1815.6
-
 NH4+ pH 8.5, 30°C Acetivibrio thermocellus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.1.16 8.6
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Bacteroides fragilis
1.4.1.16 23.8
-
 NH4+ pH 8.5, 30°C Corynebacterium glutamicum
1.4.1.16 38.3
-
 NH4+ pH 8.5, 30°C Bacteroides fragilis
1.4.1.16 41.2
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Corynebacterium glutamicum
1.4.1.16 47.4
-
 NH4+ pH 8.5, 30°C Lysinibacillus sphaericus
1.4.1.16 53.4
-
 NH4+ pH 8.5, 30°C Ureibacillus thermosphaericus
1.4.1.16 61.5
-
 NH4+ pH 8.5, 30°C Symbiobacterium thermophilum
1.4.1.16 64.3
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Lysinibacillus sphaericus
1.4.1.16 64.9
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Ureibacillus thermosphaericus
1.4.1.16 70
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Bacteroides fragilis
1.4.1.16 80.9
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Symbiobacterium thermophilum
1.4.1.16 155
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Corynebacterium glutamicum
1.4.1.16 194.6
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Ureibacillus thermosphaericus
1.4.1.16 205.4
-
 NH4+ pH 8.5, 30°C Acetivibrio thermocellus
1.4.1.16 220
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Symbiobacterium thermophilum
1.4.1.16 281.3
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Lysinibacillus sphaericus
1.4.1.16 1638.6
-
 meso-2,6-diaminoheptanedioate pH 10, 30°C Acetivibrio thermocellus
1.4.1.16 2318.3
-
 L-DELTA1-tetrahydrodipicolinate pH 8.5, 30°C Acetivibrio thermocellus