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Literature summary extracted from

  • Shimekake, Y.; Furuichi, T.; Abe, K.; Kera, Y.; Takahashi, S.
    A novel thermostable D-amino acid oxidase of the thermophilic fungus Rasamsonia emersonii strain YA (2019), Sci. Rep., 9, 11948 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3 expression in Escherichia coli Rasamsonia emersonii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 D-malate 10 mM, 13.5% inhibition Rasamsonia emersonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 0.21
-
 D-methionine pH 8, 55°C Rasamsonia emersonii
1.4.3.3 0.63
-
 D-valine pH 8, 55°C Rasamsonia emersonii
1.4.3.3 2.7
-
 D-alanine pH 8, 55°C Rasamsonia emersonii
1.4.3.3 12.1
-
 D-glutamate pH 8, 55°C Rasamsonia emersonii
1.4.3.3 19.2
-
 D-arginine pH 8, 55°C Rasamsonia emersonii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.3 185000
-
 gel filtration Rasamsonia emersonii

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Rasamsonia emersonii A0A499UB99
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.3 70
-
 substrate D-isoleucine, 37°C, pH 8.0 Rasamsonia emersonii
1.4.3.3 112
-
 substrate D-isoleucine, 55°C, pH 8.0 Rasamsonia emersonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 cephalosporin C + H2O + O2
-
 Rasamsonia emersonii 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-alanine + H2O + O2
-
 Rasamsonia emersonii pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-allo-isoleucine + H2O + O2
-
 Rasamsonia emersonii (3R)-3-hydroxy-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-allo-threonine + H2O + O2
-
 Rasamsonia emersonii ? + NH3 + H2O2
-
 ?
1.4.3.3 D-arginine + H2O + O2
-
 Rasamsonia emersonii 5-guanidino-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-glutamate + H2O + O2
-
 Rasamsonia emersonii 2-oxoglutarate + NH3 + H2O2
-
 ?
1.4.3.3 D-glutamine + H2O + O2
-
 Rasamsonia emersonii 2-oxoglutaramate + NH3 + H2O2
-
 ?
1.4.3.3 D-histidine + H2O + O2
-
 Rasamsonia emersonii 3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-isoleucine + H2O + O2
-
 Rasamsonia emersonii 3-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-leucine + H2O + O2
-
 Rasamsonia emersonii 4-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-methionine + H2O + O2
-
 Rasamsonia emersonii 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2
-
 Rasamsonia emersonii phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-serine + H2O + O2
-
 Rasamsonia emersonii 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?
1.4.3.3 D-tyrosine + H2O + O2
-
 Rasamsonia emersonii 4-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-valine + H2O + O2
-
 Rasamsonia emersonii 2-oxoisovalerate + NH3 + H2O2
-
 ?
1.4.3.3 additional information no substrates: L-isoleucine, L-valine, L-methionine Rasamsonia emersonii ?
-
-

Subunits

EC Number Subunits Comment Organism
1.4.3.3 tetramer 4 * 42000, SDS-PAGE Rasamsonia emersonii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.3 55
-
-
 Rasamsonia emersonii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.3 50
-
 stable for 1 h Rasamsonia emersonii
1.4.3.3 60
-
 T50 value Rasamsonia emersonii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 45.6
-
 D-arginine pH 8, 55°C Rasamsonia emersonii
1.4.3.3 90.7
-
 D-glutamate pH 8, 55°C Rasamsonia emersonii
1.4.3.3 120
-
 D-methionine pH 8, 55°C Rasamsonia emersonii
1.4.3.3 189
-
 D-alanine pH 8, 55°C Rasamsonia emersonii
1.4.3.3 225
-
 D-valine pH 8, 55°C Rasamsonia emersonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.3 8
-
-
 Rasamsonia emersonii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.3 6 10 stable for at least 1 h Rasamsonia emersonii

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD
-
 Rasamsonia emersonii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.3 2.38
-
 D-arginine pH 8, 55°C Rasamsonia emersonii
1.4.3.3 7.49
-
 D-glutamate pH 8, 55°C Rasamsonia emersonii
1.4.3.3 69.9
-
 D-alanine pH 8, 55°C Rasamsonia emersonii
1.4.3.3 358
-
 D-valine pH 8, 55°C Rasamsonia emersonii
1.4.3.3 562
-
 D-methionine pH 8, 55°C Rasamsonia emersonii