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Literature summary extracted from

  • Kreamer, N.; Chopra, R.; Caughlan, R.; Fabbro, D.; Fang, E.; Gee, P.; Hunt, I.; Li, M.; Leon, B.; Muller, L.; Vash, B.; Woods, A.; Stams, T.; Dean, C.; Uehara, T.
    Acylated-acyl carrier protein stabilizes the Pseudomonas aeruginosa WaaP lipopolysaccharide heptose kinase (2018), Sci. Rep., 8, 14124 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.235 myristoyl-ACP myristoyl-ACP is the likely physiological binding partner for WaaP Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.235 expression in Escherichia coli. WaaP produced by in vitro transcription-translation is insoluble unless acyl-ACP is present Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.235 structure in complex with acylated-acyl carrier protein (acyl-ACP) Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.235 A214F mutation in mid pocket, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 A214L mutation in mid pocket, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 A214W mutation in mid pocket, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 D163A mutation in HRD motif, no growth in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 D188N mutation in ATP binding, no growth in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 E78A mutation in ATP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 H161A mutation in HRD motif, no growth in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 H168A mutation in substrate binding, no growth in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 K51A mutation in ATP binding, no growth in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 K69A mutation in substrate binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 L143W mutation in lipid terminus, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 L219W mutation in mid pocket, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 L228A mutation in pocket proximal to phosphopentatheine, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 L228W mutation in pocket proximal to phosphopentatheine, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 additional information WaaP produced by in vitro transcription-translation is insoluble unless acyl-ACP is present. WaaP variants designed to perturb the acyl-ACP interaction are less stable in cells and exhibit reduced kinase function Pseudomonas aeruginosa
2.7.1.235 R162A mutation in HRD motif, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 R191A mutation in substrate binding, no growth in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 R221E/R222E/R226E/R229E/R237E mutation in ACP binding, no growth in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 R222A mutation in ACP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 R222A/R226A mutation in ACP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 R222E mutation in ACP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 R222E/R226E mutation in ACP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 R222E/R226E/R237E mutation in ACP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 R226A mutation in ACP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 R226E mutation in ACP binding, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 S127L mutation in lipid terminus, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 T198A mutation in phosphorylation site, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 V147L mutation in mid pocket, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 V147L/L219W mutation in mid pocket, able to grow in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 V147W mutation in mid pocket, no growth in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 V147W/214F mutation in mid pocket, no growth in an EDTA resistance assay Pseudomonas aeruginosa
2.7.1.235 Y165A mutation in substrate binding, no growth in an EDTA resistance assay Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.235 Pseudomonas aeruginosa Q9HUF7 cf. EC 2.7.10.2
-
2.7.1.235 Pseudomonas aeruginosa DSM 22644 Q9HUF7 cf. EC 2.7.10.2
-

Synonyms

EC Number Synonyms Comment Organism
2.7.1.235 RfaP
-
 Pseudomonas aeruginosa
2.7.1.235 WaaP
-
 Pseudomonas aeruginosa