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Literature summary extracted from

  • Srivastava, A.; Singh, V.; Haque, S.; Pandey, S.; Mishra, M.; Jawed, A.; Shukla, P.K.; Singh, P.K.; Tripathi, C.K.M.
    Response surface methodology-genetic algorithm based medium optimization, purification, and characterization of cholesterol oxidase from Streptomyces rimosus (2018), Sci. Rep., 8, 10913 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.6 acetone 10%, 35% loss of activity Streptomyces rimosus
1.1.3.6 Ag2+ 5 mM, 45% loss of activity Streptomyces rimosus
1.1.3.6 Benzene 10%, 20% loss of activity Streptomyces rimosus
1.1.3.6 beta-mercaptoethanol 1%, 40% loss of activity Streptomyces rimosus
1.1.3.6 chloroform 10%, 80% loss of activity Streptomyces rimosus
1.1.3.6 DMSO 10%, 11% loss of activity Streptomyces rimosus
1.1.3.6 DTT 1%, 70% loss of activity Streptomyces rimosus
1.1.3.6 Hg2+ 5 mM, 50% loss of activity Streptomyces rimosus
1.1.3.6 Pb2+ 5 mM, 35% loss of activity Streptomyces rimosus
1.1.3.6 SDS 1%, 80% loss of activity Streptomyces rimosus
1.1.3.6 Triton X-100 1%, 20% loss of activity Streptomyces rimosus
1.1.3.6 Zn2+ 5 mM, 35% loss of activity Streptomyces rimosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.6 0.043
-
 cholesterol pH 7.0, 37°C Streptomyces rimosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.6 extracellular
-
 Streptomyces rimosus
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Streptomyces rimosus
-
-
-
1.1.3.6 Streptomyces rimosus MTCC 10792
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.6
-
 Streptomyces rimosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 beta-cholestanol + O2 96% activity compared to cholesterol Streptomyces rimosus ? + H2O2
-
 ?
1.1.3.6 beta-cholestanol + O2 96% activity compared to cholesterol Streptomyces rimosus MTCC 10792 ? + H2O2
-
 ?
1.1.3.6 beta-sitosterol + O2 88% activity compared to cholesterol Streptomyces rimosus ? + H2O2
-
 ?
1.1.3.6 beta-sitosterol + O2 88% activity compared to cholesterol Streptomyces rimosus MTCC 10792 ? + H2O2
-
 ?
1.1.3.6 beta-stigmasterol + O2 68% activity compared to cholesterol Streptomyces rimosus ? + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Streptomyces rimosus cholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Streptomyces rimosus MTCC 10792 cholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 dehydroepiandrosterone + O2 30% activity compared to cholesterol Streptomyces rimosus ? + H2O2
-
 ?
1.1.3.6 epiandrosterone + O2 15% activity compared to cholesterol Streptomyces rimosus ? + H2O2
-
 ?
1.1.3.6 ergosterol + O2 12% activity compared to cholesterol Streptomyces rimosus ? + H2O2
-
 ?
1.1.3.6 ergosterol + O2 12% activity compared to cholesterol Streptomyces rimosus MTCC 10792 ? + H2O2
-
 ?
1.1.3.6 pregnenolone + O2 46% activity compared to cholesterol Streptomyces rimosus ? + H2O2
-
 ?
1.1.3.6 pregnenolone + O2 46% activity compared to cholesterol Streptomyces rimosus MTCC 10792 ? + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.6 40
-
-
 Streptomyces rimosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.6 20 50 20°C: about 45% of maximal activity, 50°C: about 35% of maximal activity Streptomyces rimosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.6 40
-
 stable at Streptomyces rimosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 7
-
-
 Streptomyces rimosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.6 6 9 pH 6.0: about 50% of maximal activity, pH 9.0: about 40% of maximal activity Streptomyces rimosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.6 7
-
 stable at Streptomyces rimosus