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Literature summary extracted from

  • Zhang, W.; Zhang, M.; Gao, C.; Zhang, Y.; Ge, Y.; Guo, S.; Guo, X.; Zhou, Z.; Liu, Q.; Zhang, Y.; Ma, C.; Tao, F.; Xu, P.
    Coupling between D-3-phosphoglycerate dehydrogenase and D-2-hydroxyglutarate dehydrogenase drives bacterial L-serine synthesis (2017), Proc. Natl. Acad. Sci. USA, 114, E7574-E7582 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.39 expressed as a His-tagged protein Pseudomonas stutzeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.39 0.00771
-
 electron transfer flavoprotein pH 7.4, 37°C Pseudomonas stutzeri
1.1.99.39 0.17
-
 (R)-2-hydroxyglutarate pH 7.4, 37°C Pseudomonas stutzeri
1.1.99.39 0.21
-
 cytochrome c pH 7.4, 37°C Pseudomonas stutzeri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.95 cytoplasm
-
 Pseudomonas stutzeri 5737
-
1.1.1.95 cytoplasm
-
 Pseudomonas aeruginosa 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.95 3-phospho-D-glycerate + NAD+ Pseudomonas stutzeri
-
 3-phosphooxypyruvate + NADH + H+
-
 r
1.1.1.95 3-phospho-D-glycerate + NAD+ Pseudomonas aeruginosa
-
 3-phosphooxypyruvate + NADH + H+
-
 r
1.1.1.95 3-phospho-D-glycerate + NAD+ Pseudomonas stutzeri A1501
-
 3-phosphooxypyruvate + NADH + H+
-
 r
1.1.99.39 (R)-2-hydroxyglutarate + acceptor Pseudomonas stutzeri
-
 2-oxoglutarate + reduced acceptor
-
 ?
1.1.99.39 (R)-2-hydroxyglutarate + acceptor Pseudomonas stutzeri A1501
-
 2-oxoglutarate + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.95 Pseudomonas aeruginosa Q9I6H5
-
-
1.1.1.95 Pseudomonas stutzeri A4VGK3
-
-
1.1.1.95 Pseudomonas stutzeri A1501 A4VGK3
-
-
1.1.99.39 Pseudomonas stutzeri
-
-
-
1.1.99.39 Pseudomonas stutzeri A1501
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.39
-
 Pseudomonas stutzeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.95 3-phospho-D-glycerate + NAD+
-
 Pseudomonas stutzeri 3-phosphooxypyruvate + NADH + H+
-
 r
1.1.1.95 3-phospho-D-glycerate + NAD+
-
 Pseudomonas aeruginosa 3-phosphooxypyruvate + NADH + H+
-
 r
1.1.1.95 3-phospho-D-glycerate + NAD+
-
 Pseudomonas stutzeri A1501 3-phosphooxypyruvate + NADH + H+
-
 r
1.1.99.39 (R)-2-hydroxyglutarate + acceptor
-
 Pseudomonas stutzeri 2-oxoglutarate + reduced acceptor
-
 ?
1.1.99.39 (R)-2-hydroxyglutarate + acceptor
-
 Pseudomonas stutzeri A1501 2-oxoglutarate + reduced acceptor
-
 ?
1.1.99.39 (R)-2-hydroxyglutarate + cytochrome c
-
 Pseudomonas stutzeri 2-oxoglutarate + reduced cytochrome c
-
 ?
1.1.99.39 (R)-2-hydroxyglutarate + cytochrome c
-
 Pseudomonas stutzeri A1501 2-oxoglutarate + reduced cytochrome c
-
 ?
1.1.99.39 (R)-2-hydroxyglutarate + electron transfer flavoprotein
-
 Pseudomonas stutzeri 2-oxoglutarate + reduced electron transfer flavoprotein
-
 ?
1.1.99.39 (R)-2-hydroxyglutarate + electron transfer flavoprotein
-
 Pseudomonas stutzeri A1501 2-oxoglutarate + reduced electron transfer flavoprotein
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.39 homodimer
-
 Pseudomonas stutzeri

Synonyms

EC Number Synonyms Comment Organism
1.1.1.95 D-3-PG dehydrogenase
-
 Pseudomonas stutzeri
1.1.1.95 D-3-PG dehydrogenase
-
 Pseudomonas aeruginosa
1.1.1.95 D-3-phosphoglycerate dehydrogenase
-
 Pseudomonas stutzeri
1.1.1.95 D-3-phosphoglycerate dehydrogenase
-
 Pseudomonas aeruginosa
1.1.1.95 Phgdh
-
 Pseudomonas stutzeri
1.1.1.95 Phgdh
-
 Pseudomonas aeruginosa
1.1.1.95 serA
-
 Pseudomonas stutzeri
1.1.1.95 serA
-
 Pseudomonas aeruginosa
1.1.99.39 D2HGDH
-
 Pseudomonas stutzeri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.39 1.46
-
 cytochrome c pH 7.4, 37°C Pseudomonas stutzeri
1.1.99.39 7.9
-
 (R)-2-hydroxyglutarate pH 7.4, 37°C Pseudomonas stutzeri
1.1.99.39 12.98
-
 electron transfer flavoprotein pH 7.4, 37°C Pseudomonas stutzeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.39 FAD the enzyme binds one flavin adenine dinucleotide Pseudomonas stutzeri

General Information

EC Number General Information Comment Organism
1.1.1.95 metabolism key enzyme in L-serine biosynthesis. The coupling between D-3-phosphoglycerate dehydrogenase (SerA) and D-2-hydroxyglutarate dehydrogenase (D2HGDH) sits in the hub of L-serine biosynthesis and establishes a linkage among intermediates in glycolysis, TCA cycle, and amino acid biosynthesis. The reactions catalyzed by cytoplasmic SerA and mitochondrial D2HGDH may be coupled through the shuttle of 2-ketoglutarate and D-2-hydroxyglutarate between the cytosol and mitochondrial matrix Pseudomonas stutzeri
1.1.1.95 metabolism key enzyme in L-serine biosynthesis. The coupling between D-3-phosphoglycerate dehydrogenase (SerA) and D-2-hydroxyglutarate dehydrogenase (D2HGDH) sits in the hub of L-serine biosynthesis and establishes a linkage among intermediates in glycolysis, TCA cycle, and amino acid biosynthesis. The reactions catalyzed by cytoplasmic SerA and mitochondrial D2HGDH may be coupled through the shuttle of 2-ketoglutarate and D-2-hydroxyglutarate between the cytosol and mitochondrial matrix Pseudomonas aeruginosa
1.1.99.39 malfunction deletion mutant displays decreased growth. The defect is rescued by adding L-serine Pseudomonas stutzeri
1.1.99.39 physiological function the enzyme is functionally tied to L-serine synthesis. D-2-Hydroxyglutarate-mediated coupling between D-3-phosphoglycerate dehydrogenase (SerA) and D-2-hydroxyglutarate dehydrogenase drives bacterial L-serine synthesis Pseudomonas stutzeri

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.99.39 6.9
-
 cytochrome c pH 7.4, 37°C Pseudomonas stutzeri
1.1.99.39 45.38
-
 (R)-2-hydroxyglutarate pH 7.4, 37°C Pseudomonas stutzeri
1.1.99.39 1716.7
-
 electron transfer flavoprotein pH 7.4, 37°C Pseudomonas stutzeri