EC Number | Application | Comment | Organism |
---|---|---|---|
1.3.5.5 | agriculture | phytoene desaturase is a prominent target of certain inhibitors, such as norflurazon, acting as bleaching herbicides | Oryza sativa Indica Group |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.5.5 | gene OsI_010044 or PDS1, recombinant expression of wild-type and mutant His6-tagged enzymes OsPDS in Escherichia coli | Oryza sativa Indica Group |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.5.5 | F162V | -directed mutagenesis, the mutant shows below 5% activity compared to wild-type enzyme | Oryza sativa Indica Group |
1.3.5.5 | L538F | -directed mutagenesis, the mutant shows below 5% activity compared to wild-type enzyme | Oryza sativa Indica Group |
1.3.5.5 | L538R | -directed mutagenesis, the mutant shows below 5% activity compared to wild-type enzyme | Oryza sativa Indica Group |
1.3.5.5 | R300S | -directed mutagenesis, the mutant shows below 5% activity compared to wild-type enzyme | Oryza sativa Indica Group |
1.3.5.5 | R300T | site-directed mutagenesis, the mutant shows below 5% activity compared to wild-type enzyme | Oryza sativa Indica Group |
1.3.5.5 | T508V | -directed mutagenesis, the mutant shows below 5% activity compared to wild-type enzyme | Oryza sativa Indica Group |
1.3.5.5 | Y506F | -directed mutagenesis, the mutant shows below 5% activity compared to wild-type enzyme | Oryza sativa Indica Group |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.5 | norflurazon | NFZ, competitive inhibition, inhibition kinetics with enzyme mutant R300S | Oryza sativa Indica Group |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.5.5 | additional information | - |
additional information | Michaelis-Menten kinetics | Oryza sativa Indica Group |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.5.5 | 15,9'-dicis-phytofluene + plastoquinone | Oryza sativa Indica Group | - |
9,15,9'-tricis-zeta-carotene + plastoquinol | - |
? | |
1.3.5.5 | 15-cis-phytoene + plastoquinone | Oryza sativa Indica Group | - |
15,9'-dicis-phytofluene + plastoquinol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.5.5 | Oryza sativa Indica Group | A2XDA1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.5.5 | recombinant wild-type and mutant His6-tagged enzymes OsPDS from Escherichia coli by nickel affinity chromatography | Oryza sativa Indica Group |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.3.5.5 | 15-cis-phytoene + 2 plastoquinone = 9,15,9'-tricis-zeta-carotene + 2 plastoquinol | overall reaction, ordered ping-pong bi-bi mechanism, both lipophilic substrates are bound inside the same substrate cavity that cannot be occupied by both simultaneously. The central 15-cis configuration of phytoene mediates the regiospecificity in catalysis | Oryza sativa Indica Group |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.5.5 | 15,9'-dicis-phytofluene + plastoquinone | - |
Oryza sativa Indica Group | 9,15,9'-tricis-zeta-carotene + plastoquinol | - |
? | |
1.3.5.5 | 15-cis-phytoene + plastoquinone | - |
Oryza sativa Indica Group | 15,9'-dicis-phytofluene + plastoquinol | - |
? | |
1.3.5.5 | 15-cis-phytoene + plastoquinone | decyl-plastoquinone is used for enzyme assays | Oryza sativa Indica Group | 15,9'-dicis-phytofluene + plastoquinol | - |
? | |
1.3.5.5 | additional information | PDS requires plastoquinone as a directly interacting co-substrate to reoxidize the enzyme-bound FADred formed upon desaturation. The enzyme structure has led to the conclusion that both lipophilic substrates are bound inside the same substrate cavity that cannot be occupied by both simultaneously. The central 15-cis configuration of phytoene mediates the regiospecificity in catalysis, overview. LC-MS analysis of PDS desaturation products produced from asymmetric (C35) 15-cis-norphytoene | Oryza sativa Indica Group | ? | - |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.5.5 | OsPDS | - |
Oryza sativa Indica Group |
1.3.5.5 | PDS | - |
Oryza sativa Indica Group |
1.3.5.5 | PDS1 | - |
Oryza sativa Indica Group |
1.3.5.5 | phytoene desaturase | - |
Oryza sativa Indica Group |
1.3.5.5 | plant-type phytoene desaturase | - |
Oryza sativa Indica Group |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.5.5 | 35 | - |
- |
Oryza sativa Indica Group |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.5.5 | 6.5 | - |
- |
Oryza sativa Indica Group |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.5 | FAD | PDS requires plastoquinone as a directly interacting co-substrate to reoxidize the enzyme-bound FADred formed upon desaturation | Oryza sativa Indica Group |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.3.5.5 | additional information | using the crystal structure of Oryza sativa PDS in complex with norflurazon, the relevance of homotetrameric assembly of the enzyme observed in crystallo is determined by dynamic mathematical modeling of reaction time courses, substrate channeling occurs of the intermediate phytofluene between individual subunits at membrane surfaces | Oryza sativa Indica Group |
1.3.5.5 | physiological function | phytoene desaturase (PDS) is an essential plant carotenoid biosynthetic enzyme. PDS catalyzes the introduction of two double bonds into 15-cis-phytoene, yielding 9,15,9'-tri-cis-zeta-carotene via the intermediate 9,15-di-cis-phytofluene | Oryza sativa Indica Group |