EC Number | Application | Comment | Organism |
---|---|---|---|
1.2.3.4 | analysis | membrane inlet mass spectrometry assay to directly measure initial rates of carbon dioxide formation and oxygen consumption in the presence and absence of hydrogen peroxide | Gelatoporia subvermispora |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.3.4 | expression in Pichia pastoris | Gelatoporia subvermispora |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.4 | H2O2 | hydrogen peroxide is both a reversible noncompetitive inhibitor of the OxOx catalyzed oxidation of oxalate and an irreversible inactivator. The build-up of the turnover-generated hydrogen peroxide product leads to the inactivation of the enzyme. The introduction of catalase to reaction mixtures protects the enzyme from inactivation allowing reactions to proceed to completion. No changes in global protein structure take place in the presence of hydrogen peroxide | Gelatoporia subvermispora |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.3.4 | 0.47 | - |
oxalate | pH 4, 25°C | Gelatoporia subvermispora | |
1.2.3.4 | 0.47 | - |
oxalate | presence of 2 mM H2O2, pH 4, 25°C | Gelatoporia subvermispora | |
1.2.3.4 | 0.56 | - |
oxalate | presence of 4 mM H2O2, pH 4, 25°C | Gelatoporia subvermispora | |
1.2.3.4 | 0.76 | - |
oxalate | presence of 10 mM H2O2, pH 4, 25°C | Gelatoporia subvermispora |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.3.4 | Gelatoporia subvermispora | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.3.4 | mesoxalate + O2 + 2 H+ | - |
Gelatoporia subvermispora | CO2 + oxalate + H2O2 | - |
? | |
1.2.3.4 | oxalate + O2 + 2 H+ | - |
Gelatoporia subvermispora | 2 CO2 + 2 H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.3.4 | OxOx | - |
Gelatoporia subvermispora |