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Literature summary extracted from

  • Guirimand, G.; Guihur, A.; Perello, C.; Phillips, M.; Mahroug, S.; Oudin, A.; Duge de Bernonville, T.; Besseau, S.; Lanoue, A.; Giglioli-Guivarch, N.; Papon, N.; St-Pierre, B.; Rodriguez-Concepcion, M.; Burlat, V.; Courdavault, V.
    Cellular and subcellular compartmentation of the 2C-methyl-D-erythritol 4-phosphate pathway in the Madagascar periwinkle (2020), Plants (Basel), 9, 462 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.267 gene DXR, recombinant expression of YFP-tagged enzyme DXR in Catharanthus roseus cells Catharanthus roseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.267 chloroplast diffuse in stroma and stromules of plastids, immunogold labeling of DXR in the stroma of plastids from Catharanthus roseus leaf cells Catharanthus roseus 9507
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1.1.1.267 additional information enzyme 1-deoxy-D-xylulose 5-phosphate reductoisomerase exhibits an artifactual aggregated pattern of localization due to high protein accumulation. It is rather diffuse in stroma and stromules of plastids, subcellular localization study, MEP pathway gene RNA seq coexpression pattern, overview Catharanthus roseus
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+ Catharanthus roseus
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 2-C-methyl-D-erythritol 4-phosphate + NADP+
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.267 Catharanthus roseus
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.267 CR6 cell
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 Catharanthus roseus
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1.1.1.267 leaf all MEP pathway genes are coordinately and mainly expressed in internal phloem-associated parenchyma of young leaves Catharanthus roseus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.267 1-deoxy-D-xylulose 5-phosphate + NADPH + H+
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 Catharanthus roseus 2-C-methyl-D-erythritol 4-phosphate + NADP+
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 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.267 1-deoxy-D-xylulose 5-phosphate reductoisomerase
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 Catharanthus roseus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.267 NADP+
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 Catharanthus roseus
1.1.1.267 NADPH
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 Catharanthus roseus

General Information

EC Number General Information Comment Organism
1.1.1.267 metabolism the enzyme is involved in the 2C-methyl-D-erythritol 4-phosphate (MEP) pathway in leaves. The MEP pathway initiates the biosynthesis of highly valuable monoterpene indole alkaloids (MIAs). The MIA biosynthetic pathway shows in leaves a complex compartmentation occuring at both the cellular and subcellular levels, notably for some gene products of the MEP pathway. All MEP pathway genes are coordinately and mainly expressed in internal phloem-associated parenchyma of young leaves, reinforcing the role of this tissue in MIA biosynthesis. A potential role of stromules in enhancing MIA precursor exchange with other cell compartments to favor metabolic fluxes towards the MIA biosynthesis. The MEP pathway produces both isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) through seven enzymatic reactions initiated by the synthesis of 1-deoxy-D-xylulose 5-phosphate (DXP) from pyruvate and glyceraldehyde 3-phosphate. This reaction is catalyzed by DXP synthase (DXS), encoded by a small gene family in higher plants. The DXS isogenes have been clustered into two related gene groups: Clade I-DXS including housekeeping genes and Clade II-DXS including genes associated with plant defense and secondary metabolism. DXP is then sequentially converted into MEP by DXP reductoisomerase (DXR) and into 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (CDP-ME) following the addition of cytidine triphosphate by CDP-ME synthase (CMS), pathway overview. MEP pathway enzymes are targeted to plastids Catharanthus roseus