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Literature summary extracted from

  • Lee, H.J.; Nakayasu, M.; Akiyama, R.; Kobayashi, M.; Miyachi, H.; Sugimoto, Y.; Umemoto, N.; Saito, K.; Muranaka, T.; Mizutani, M.
    Identification of a 3beta-hydroxysteroid dehydrogenase/ 3-ketosteroid reductase involved in alpha-tomatine biosynthesis in tomato (2019), Plant Cell Physiol., 60, 1304-1315 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.145 overexpression of the enzyme (Sl3bHSD1) in potato hairy roots Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.145 0.02402
-
dehydrotomatidine pH 7.2, 30°C Solanum lycopersicum
1.1.1.145 0.04534
-
pregnenolone pH 7.2, 30°C Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Solanum lycopersicum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.145 flower high expression Solanum lycopersicum
-
1.1.1.145 fruit high expression Solanum lycopersicum
-
1.1.1.145 leaf high expression Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 dehydrotomatidine + NADH + H+
-
Solanum lycopersicum tomatid-4-en-3-one + NAD+
-
?
1.1.1.145 pregnenolone + NAD+
-
Solanum lycopersicum progesterone + NADH + H+
-
?
1.1.1.145 tomatid-3-one + NAD+
-
Solanum lycopersicum tomatidine + NADH + H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.145 3beta-hydroxysteroid dehydrogenase/3-ketosteroid reductase
-
Solanum lycopersicum
1.1.1.145 Sl3betaHSD1
-
Solanum lycopersicum
1.1.1.145 Solyc01g73640
-
Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.145 1145.21
-
pregnenolone pH 7.2, 30°C Solanum lycopersicum
1.1.1.145 2769.17
-
dehydrotomatidine pH 7.2, 30°C Solanum lycopersicum

General Information

EC Number General Information Comment Organism
1.1.1.145 metabolism key enzyme involved in the conversion processes from dehydrotomatidine to tomatidine. The enzyme is involved in alpha-tomatine biosynthesis. alpha-Tomatine and dehydrotomatine are major steroidal glycoalkaloids that accumulate in the mature green fruits, leaves and flowers of tomato (Solanum lycopersicum), and function as defensive compounds against bacteria, fungi, insects and animals Solanum lycopersicum