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Literature summary extracted from

  • Nisa, K.; Ashraf, S.; Siddiqui, M.; Taj, N.; Habib-Ur-Rehma, H.; Bano, A.; Rashid, N.
    Purification and characterization of a thermostable pyruvate ferredoxin oxidoreductase/pyruvate decarboxylase from Thermococcus kodakaraensis (2020), Pak. J. Zool., 52, 1149-1156 .
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.1 0.00049
-
 pyruvate pH 8.5, 85°C Thermococcus kodakarensis
1.2.7.1 0.115
-
 CoA pH 8.5, 85°C Thermococcus kodakarensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.1 240000
-
 gel filtration Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.1 Thermococcus kodakarensis
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.1 activity is rapidly lost under aerobic conditions Thermococcus kodakarensis

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.1 purifiaction using Q-sepharose, hydroxyapatite, blue sepharose and sephacryl Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.1 pyruvate + CoA + 2 oxidized methyl viologen
-
 Thermococcus kodakarensis acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.1 octamer dimer of heterotetramers (alphabetagammadelta)2, 2 * 44000, alpha-subunit, 2 * 36000, beta-subunit, 2 * 20000, gamma subunit, 2 * 12000, delta-subunit, SDS-PAGE Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.1 95
-
-
 Thermococcus kodakarensis