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Literature summary extracted from

  • Sugai, Y.; Katsuyama, Y.; Ohnishi, Y.
    A nitrous acid biosynthetic pathway for diazo group formation in bacteria (2016), Nat. Chem. Biol., 12, 73-75 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.99.5 expressed in Escherichia coli BL21(DE3) cells Streptomyces cremeus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.248 0.055
-
 NADPH 30°C, pH not specified in the publication Streptomyces cremeus
1.14.13.248 0.125
-
 L-aspartate 30°C, pH not specified in the publication Streptomyces cremeus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.248 (2S)-2-nitrosobutanedioate + NADPH + H+ + O2 Streptomyces cremeus
-
 (2S)-2-nitrobutanedioate + NADP+ + H2O
-
 ?
1.14.13.248 L-aspartate + 3 NADPH + 3 H+ + 3 O2 Streptomyces cremeus
-
 (2S)-2-nitrobutanedioate + 3 NADP+ + 4 H2O
-
 ?
1.14.13.248 L-aspartate + NADPH + H+ + O2 Streptomyces cremeus
-
 N-hydroxy-L-aspartate + NADP+ + H2O
-
 ?
1.14.13.248 N-hydroxy-L-aspartate + NADPH + H+ + O2 Streptomyces cremeus
-
 N,N-dihydroxy-L-aspartate + NADP+ + H2O
-
 ?
1.14.13.249 3-amino-4-hydroxybenzoate + NADPH + H+ + O2 Streptomyces cremeus
-
 3-amino-2,4-dihydroxybenzoate + NADP+ + H2O
-
 ?
4.3.99.5 nitrosuccinic acid Streptomyces cremeus the enzyme accepts nitrosuccinic acid as a substrate only when it is directly delivered by the enzyme CreE immediately after its synthesis fumaric acid + nitrous acid
-
 ?
4.3.99.5 nitrosuccinic acid Streptomyces cremeus NBRC 12760 the enzyme accepts nitrosuccinic acid as a substrate only when it is directly delivered by the enzyme CreE immediately after its synthesis fumaric acid + nitrous acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.248 Streptomyces cremeus A0A0K2JL70
-
-
1.14.13.249 Streptomyces cremeus A0A0K2JKU1
-
-
4.3.99.5 Streptomyces cremeus
-
-
-
4.3.99.5 Streptomyces cremeus NBRC 12760
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.99.5 Cosmogel His-Accept column chromatography Streptomyces cremeus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.248 (2S)-2-nitrosobutanedioate + NADPH + H+ + O2
-
 Streptomyces cremeus (2S)-2-nitrobutanedioate + NADP+ + H2O
-
 ?
1.14.13.248 L-aspartate + 3 NADPH + 3 H+ + 3 O2
-
 Streptomyces cremeus (2S)-2-nitrobutanedioate + 3 NADP+ + 4 H2O
-
 ?
1.14.13.248 L-aspartate + NADPH + H+ + O2
-
 Streptomyces cremeus N-hydroxy-L-aspartate + NADP+ + H2O
-
 ?
1.14.13.248 N-hydroxy-L-aspartate + NADPH + H+ + O2
-
 Streptomyces cremeus N,N-dihydroxy-L-aspartate + NADP+ + H2O
-
 ?
1.14.13.249 3-amino-4-hydroxybenzoate + NADPH + H+ + O2
-
 Streptomyces cremeus 3-amino-2,4-dihydroxybenzoate + NADP+ + H2O
-
 ?
1.14.13.249 3-amino-4-methoxybenzoate + NADPH + H+ + O2
-
 Streptomyces cremeus 3-amino-2-hydroxy-4-methoxybenzoate + NADP+ + H2O
-
 ?
4.3.99.5 nitrosuccinic acid the enzyme accepts nitrosuccinic acid as a substrate only when it is directly delivered by the enzyme CreE immediately after its synthesis Streptomyces cremeus fumaric acid + nitrous acid
-
 ?
4.3.99.5 nitrosuccinic acid the enzyme accepts nitrosuccinic acid as a substrate only when it is directly delivered by the enzyme CreE immediately after its synthesis Streptomyces cremeus NBRC 12760 fumaric acid + nitrous acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.248 creE
-
 Streptomyces cremeus
4.3.99.5 creD
-
 Streptomyces cremeus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.248 1.14
-
 L-aspartate 30°C, pH not specified in the publication Streptomyces cremeus
1.14.13.248 1.19
-
 NADPH 30°C, pH not specified in the publication Streptomyces cremeus

General Information

EC Number General Information Comment Organism
1.14.13.248 metabolism CreE catalyzes iterative oxidation of L-aspartic acid to nitrosuccinic acid via N-hydroxyaspartic acid and nitrososuccinic acid. When CreE and CreD are incubated with L-aspartic acid, nitrous acid is the product. CreD accepts nitrosuccinic acid as a substrate only when it is directly delivered by CreE immediately after its synthesis. Both are involved in cremeomycin biosynthesis Streptomyces cremeus