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Literature summary extracted from

  • Mayol, O.; Bastard, K.; Beloti, L.; Frese, A.; Turkenburg, J.P.; Petit, J.-L.; Mariage, A.; Debard, A.; Pellouin, V.; Perret, A.; de Berardinis, V.; Zaparucha, A.; Grogan, G.; Vergne-Vaxelaire, C.
    A family of native amine dehydrogenases for the asymmetric reductive amination of ketones (2019), Nat. Catal., 2, 324 .
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.28 structure without substrate but bound to the cofactor NAD+, and a model of the ternary complex with product (2R,4S)-2,4-diaminopentanoate in the closed conformation. The active site features residues Glu102, Val134, Asn135, Arg161, Asn163, Phe168, Val172, Gln176, His197, Ile198, His264, Gln266, Gly299 and Thr303 Petrotoga mobilis

Protein Variants

EC Number Protein Variants Comment Organism
1.4.1.28 E102A residue Glu102 is involved in the activation of ammonia, mutation significantly decreases activity Petrotoga mobilis
1.4.1.28 R161M mutant gains activity with substrate pentan-2-one Petrotoga mobilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.28 2-methylcyclohexanone
-
 Microbacterium sp. MA1
1.4.1.28 2-methylcyclohexanone
-
 Mycolicibacterium smegmatis
1.4.1.28 2-methylcyclohexanone
-
 Petrotoga mobilis
1.4.1.28 cyclohexanone substrate inhibition Cystobacter fuscus
1.4.1.28 cyclohexanone
-
 Microbacterium sp. MA1
1.4.1.28 cyclohexanone
-
 Mycolicibacterium smegmatis
1.4.1.28 cyclohexanone
-
 Petrotoga mobilis
1.4.1.28 Isobutanal
-
 Microbacterium sp. MA1
1.4.1.28 Isobutanal
-
 Mycolicibacterium smegmatis
1.4.1.28 Isobutanal
-
 Petrotoga mobilis
1.4.1.28 NH3 substrate inhibition Cystobacter fuscus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.28 0.01
-
 NADPH pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.025
-
 NADH wild-type, pH 9.5, 23°C Petrotoga mobilis
1.4.1.28 0.06
-
 NADPH pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.06
-
 NADH pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.29
-
 NADH pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.326
-
 (2R)-2-amino-4-oxopentanoate wild-type, pH 9.5, 23°C Petrotoga mobilis
1.4.1.28 0.54
-
 Isobutanal pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.55
-
 cyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.86
-
 Isobutanal pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.894
-
 (2R)-2-amino-4-oxopentanoate mutant E102A, pH 9.5, 23°C Petrotoga mobilis
1.4.1.28 2.32
-
 2-methylcyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 2.47
-
 cyclohexanone pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 98.94
-
 NH3 pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 319.7
-
 NH3 wild-type, pH 9.5, 23°C Petrotoga mobilis
1.4.1.28 392.2
-
 NH3 pH 9.5, 23°C Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.28 (2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+ Petrotoga mobilis
-
 (2R,4S)-2,4-diaminopentanoate + NAD+ + H2O
-
 ?
1.4.1.28 (2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+ Petrotoga mobilis DSM 10674
-
 (2R,4S)-2,4-diaminopentanoate + NAD+ + H2O
-
 ?
1.4.1.28 pentan-2-one + NH3 + NADH + H+ Petrotoga mobilis no substrate of wild-type, but substrate of mutants carrying substitution R161M pentan-2-amine + NAD+ + H2O
-
 ?
1.4.1.28 pentan-2-one + NH3 + NADH + H+ Petrotoga mobilis DSM 10674 no substrate of wild-type, but substrate of mutants carrying substitution R161M pentan-2-amine + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.28 Cystobacter fuscus S9Q235
-
-
1.4.1.28 Cystobacter fuscus DSM 2262 S9Q235
-
-
1.4.1.28 Microbacterium sp. MA1 C3UMY1
-
-
1.4.1.28 Mycolicibacterium smegmatis A0A8B4R7U8
-
-
1.4.1.28 Petrotoga mobilis A9BHL2
-
-
1.4.1.28 Petrotoga mobilis DSM 10674 A9BHL2
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.28 10.1
-
 substrate cyclohexanone, cosubstrate methylamine, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 16.4
-
 substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 18.6
-
 substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 19.6
-
 substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 25.1
-
 substrate cyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 25.5
-
 substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 37.3
-
 substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 54.4
-
 substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 60.2
-
 substrate cyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 73.8
-
 substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 75.2
-
 substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 75.2
-
 substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 88.1
-
 substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 88.1
-
 substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 90.4
-
 substrate cyclohexanone, cosubstrate methylamine, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 97.6
-
 substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 104.8
-
 mutant N135V/N163V/R161M/H264L, substrate pentan-2-one, 23°C, pH 9.5 Petrotoga mobilis
1.4.1.28 109.6
-
 substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 119.2
-
 substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 120.6
-
 substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 129
-
 substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 131.6
-
 substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 144.5
-
 substrate isobutanal, cosubstrate methylamine, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 159.4
-
 substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 175.2
-
 substrate 2-methylcyclohexanone, cosubstrates NH3 andNADPH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 196.2
-
 substrate cyclohexanone, cosubstrates NH3 and NADH, pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 233.2
-
 substrate cyclohexanone, cosubstrates NH3 and NADH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 245.6
-
 substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 252.6
-
 substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 257.7
-
 substrate isobutanal, cosubstrate methylamine, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 337
-
 substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 388.6
-
 substrate cyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 397.9
-
 substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 614.5
-
 substrate cyclohexanone, cosubstrates NH3 and NADH, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 851.3
-
 substrate isobutanal, cosubstrate methylamine, pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 955
-
 substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C Cystobacter fuscus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.28 (2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+
-
 Petrotoga mobilis (2R,4S)-2,4-diaminopentanoate + NAD+ + H2O
-
 ?
1.4.1.28 (2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+
-
 Petrotoga mobilis DSM 10674 (2R,4S)-2,4-diaminopentanoate + NAD+ + H2O
-
 ?
1.4.1.28 1-cyclohexylethan-1-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 1-cyclohexylethyl-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 1-cyclohexylethan-1-one + NH3 + NADH + H+
-
 Cystobacter fuscus 1-cyclohexylethyl-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 1-cyclohexylethan-1-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 1-cyclohexylethyl-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 1-cyclohexylethan-1-one + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 1-cyclohexylethyl-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 1-indanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 1-indanamine + NAD+ + H2O
-
 ?
1.4.1.28 1-indanone + NH3 + NADH + H+
-
 Cystobacter fuscus 1-indanamine + NAD+ + H2O
-
 ?
1.4.1.28 1-indanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 1-indanamine + NAD+ + H2O
-
 ?
1.4.1.28 1-indanone + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 1-indanamine + NAD+ + H2O
-
 ?
1.4.1.28 2-cyclohexen-1-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 2-cyclohexen-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 2-cyclohexen-1-one + NH3 + NADH + H+
-
 Cystobacter fuscus 2-cyclohexen-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 2-cyclohexen-1-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 2-cyclohexen-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 2-cyclohexen-1-one + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 2-cyclohexen-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 2-methylbutanal + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 2-methylbutan-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 2-methylbutanal + NH3 + NADH + H+
-
 Cystobacter fuscus 2-methylbutan-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 2-methylbutanal + NH3 + NADH + H+
-
 Microbacterium sp. MA1 2-methylbutan-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 2-methylbutanal + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 2-methylbutan-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 2-methylcyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 2-methylcyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 2-methylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus 2-methylcyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 2-methylcyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 2-methylcyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 2-methylcyclopentanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 2-methylcyclopentylamine + NAD+ + H2O
-
 ?
1.4.1.28 2-methylcyclopentanone + NH3 + NADH + H+
-
 Cystobacter fuscus 2-methylcyclopentylamine + NAD+ + H2O
-
 ?
1.4.1.28 2-methylcyclopentanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 2-methylcyclopentylamine + NAD+ + H2O
-
 ?
1.4.1.28 3,5-dimethylcyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3,5-dimethylcyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 3,5-dimethylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus 3,5-dimethylcyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 3,5-dimethylcyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3,5-dimethylcyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 3-hydroxybutan-2-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-aminobutan-2-ol + NAD+ + H2O
-
 ?
1.4.1.28 3-hydroxybutan-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus 3-aminobutan-2-ol + NAD+ + H2O
-
 ?
1.4.1.28 3-hydroxybutan-2-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3-aminobutan-2-ol + NAD+ + H2O
-
 ?
1.4.1.28 3-methylbutan-2-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-methylbutan-2-amine + NAD+ + H2O
-
 ?
1.4.1.28 3-methylbutan-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus 3-methylbutan-2-amine + NAD+ + H2O
-
 ?
1.4.1.28 3-methylbutan-2-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3-methylbutan-2-amine + NAD+ + H2O
-
 ?
1.4.1.28 3-methylcyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-methylcyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 3-methylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus 3-methylcyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 3-methylcyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3-methylcyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 3-methylcyclopentanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-methylcyclopentylamine + NAD+ + H2O
-
 ?
1.4.1.28 3-methylcyclopentanone + NH3 + NADH + H+
-
 Cystobacter fuscus 3-methylcyclopentylamine + NAD+ + H2O
-
 ?
1.4.1.28 3-methylcyclopentanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3-methylcyclopentylamine + NAD+ + H2O
-
 ?
1.4.1.28 4-hydroxybutan-2-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 3-aminobutan-1-ol + NAD+ + H2O
-
 ?
1.4.1.28 4-hydroxybutan-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus 3-aminobutan-1-ol + NAD+ + H2O
-
 ?
1.4.1.28 4-hydroxybutan-2-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 3-aminobutan-1-ol + NAD+ + H2O
-
 ?
1.4.1.28 4-methylcyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 4-methylcyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 4-methylcyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus 4-methylcyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 4-methylcyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 4-methylcyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 benzaldehyde + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis benzylamine + NAD+ + H2O
-
 ?
1.4.1.28 benzaldehyde + NH3 + NADH + H+
-
 Cystobacter fuscus benzylamine + NAD+ + H2O
-
 ?
1.4.1.28 benzaldehyde + NH3 + NADH + H+
-
 Microbacterium sp. MA1 benzylamine + NAD+ + H2O
-
 ?
1.4.1.28 but-3-en-2-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis but-3-en-2-amine + NAD+ + H2O
-
 ?
1.4.1.28 but-3-en-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus but-3-en-2-amine + NAD+ + H2O
-
 ?
1.4.1.28 but-3-en-2-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 but-3-en-2-amine + NAD+ + H2O
-
 ?
1.4.1.28 cyclohexanone + methylamine + NADH + H+
-
 Mycolicibacterium smegmatis N-methylcyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 cyclohexanone + methylamine + NADH + H+
-
 Cystobacter fuscus N-methylcyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 cyclohexanone + methylamine + NADH + H+
-
 Microbacterium sp. MA1 N-methylcyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 cyclohexanone + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis cyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 cyclohexanone + NH3 + NADH + H+
-
 Cystobacter fuscus cyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 cyclohexanone + NH3 + NADH + H+
-
 Microbacterium sp. MA1 cyclohexylamine + NAD+ + H2O
-
 ?
1.4.1.28 cyclopentanone + NH3 + NADPH + H+
-
 Mycolicibacterium smegmatis cyclopentylamine + NADP+ + H2O
-
 ?
1.4.1.28 cyclopentanone + NH3 + NADPH + H+
-
 Cystobacter fuscus cyclopentylamine + NADP+ + H2O
-
 ?
1.4.1.28 cyclopentanone + NH3 + NADPH + H+
-
 Microbacterium sp. MA1 cyclopentylamine + NADP+ + H2O
-
 ?
1.4.1.28 hexan-3-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis hexan-3-amine + NAD+ + H2O
-
 ?
1.4.1.28 hexan-3-one + NH3 + NADH + H+
-
 Cystobacter fuscus hexan-3-amine + NAD+ + H2O
-
 ?
1.4.1.28 hexan-3-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 hexan-3-amine + NAD+ + H2O
-
 ?
1.4.1.28 isobutanal + methylamine + NADH + H+
-
 Mycolicibacterium smegmatis N,2-dimethylpropan-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 isobutanal + methylamine + NADH + H+
-
 Cystobacter fuscus N,2-dimethylpropan-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 isobutanal + methylamine + NADH + H+
-
 Microbacterium sp. MA1 N,2-dimethylpropan-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 isobutanal + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis 2-methylpropan-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 isobutanal + NH3 + NADH + H+
-
 Cystobacter fuscus 2-methylpropan-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 isobutanal + NH3 + NADH + H+
-
 Microbacterium sp. MA1 2-methylpropan-1-amine + NAD+ + H2O
-
 ?
1.4.1.28 pentan-2-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis pentan-2-amine + NAD+ + H2O
-
 ?
1.4.1.28 pentan-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus pentan-2-amine + NAD+ + H2O
-
 ?
1.4.1.28 pentan-2-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 pentan-2-amine + NAD+ + H2O
-
 ?
1.4.1.28 pentan-2-one + NH3 + NADH + H+ no substrate of wild-type, but substrate of mutants carrying substitution R161M Petrotoga mobilis pentan-2-amine + NAD+ + H2O
-
 ?
1.4.1.28 pentan-2-one + NH3 + NADH + H+ no substrate of wild-type, but substrate of mutants carrying substitution R161M Petrotoga mobilis DSM 10674 pentan-2-amine + NAD+ + H2O
-
 ?
1.4.1.28 pentan-2-one + NH3 + NADH + H+
-
 Cystobacter fuscus DSM 2262 pentan-2-amine + NAD+ + H2O
-
 ?
1.4.1.28 pentan-3-one + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis pentan-3-amine + NAD+ + H2O
-
 ?
1.4.1.28 pentan-3-one + NH3 + NADH + H+
-
 Cystobacter fuscus pentan-3-amine + NAD+ + H2O
-
 ?
1.4.1.28 pentan-3-one + NH3 + NADH + H+
-
 Microbacterium sp. MA1 pentan-3-amine + NAD+ + H2O
-
 ?
1.4.1.28 pentanal + NH3 + NADH + H+
-
 Mycolicibacterium smegmatis n-pentylamine + NAD+ + H2O
-
 ?
1.4.1.28 pentanal + NH3 + NADH + H+
-
 Cystobacter fuscus n-pentylamine + NAD+ + H2O
-
 ?
1.4.1.28 pentanal + NH3 + NADH + H+
-
 Microbacterium sp. MA1 n-pentylamine + NAD+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.28 AmDH4
-
 Petrotoga mobilis
1.4.1.28 D187_009359
-
 Cystobacter fuscus
1.4.1.28 D187_009359
-
 Microbacterium sp. MA1
1.4.1.28 DapB_1
-
 Mycolicibacterium smegmatis
1.4.1.28 Pmob_1166
-
 Petrotoga mobilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.28 0.04
-
 NH3 pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.05
-
 NADPH pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.1
-
 NADH pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.11
-
 Isobutanal pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.13
-
 cyclohexanone pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.28
-
 NADPH pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.3
-
 NH3 pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.54
-
 Isobutanal pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.58
-
 NADH pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.61
-
 2-methylcyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.94
-
 cyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 2.5
-
 (2R)-2-amino-4-oxopentanoate mutant E102A, pH 9.5, 23°C Petrotoga mobilis
1.4.1.28 37.8
-
 NH3 wild-type, pH 9.5, 23°C Petrotoga mobilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.28 NADH
-
 Petrotoga mobilis
1.4.1.28 NADH specific activities for both cofactors NADH and NADPH are substrate-dependent Mycolicibacterium smegmatis
1.4.1.28 NADH specific activities for both cofactors NADH and NADPH are substrate-dependent Cystobacter fuscus
1.4.1.28 NADH specific activities for both cofactors NADH and NADPH are substrate-dependent Microbacterium sp. MA1
1.4.1.28 NADPH specific activities for both cofactors NADH and NADPH are substrate-dependent Mycolicibacterium smegmatis
1.4.1.28 NADPH specific activities for both cofactors NADH and NADPH are substrate-dependent Cystobacter fuscus
1.4.1.28 NADPH specific activities for both cofactors NADH and NADPH are substrate-dependent Microbacterium sp. MA1

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.28 0.41
-
 cyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.41
-
 cyclohexanone pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 0.41
-
 cyclohexanone pH 9.5, 23°C Petrotoga mobilis
1.4.1.28 1.99
-
 cyclohexanone pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 2.76
-
 Isobutanal pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 2.76
-
 Isobutanal pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 2.76
-
 Isobutanal pH 9.5, 23°C Petrotoga mobilis
1.4.1.28 4.94
-
 2-methylcyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 4.94
-
 2-methylcyclohexanone pH 9.5, 23°C Microbacterium sp. MA1
1.4.1.28 4.94
-
 2-methylcyclohexanone pH 9.5, 23°C Petrotoga mobilis
1.4.1.28 10623
-
 NH3 pH 9.5, 23°C Cystobacter fuscus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.1.28 0.0004
-
 NH3 pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.0008
-
 NH3 pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.054
-
 cyclohexanone pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.118
-
 NH3 wild-type, pH 9.5, 23°C Petrotoga mobilis
1.4.1.28 0.209
-
 Isobutanal pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 0.26
-
 2-methylcyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 0.62
-
 Isobutanal pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 1.705
-
 NADH pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 1.72
-
 cyclohexanone pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 1.99
-
 NADH pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 2.81
-
 (2R)-2-amino-4-oxopentanoate mutant E102A, pH 9.5, 23°C Petrotoga mobilis
1.4.1.28 4.537
-
 NADPH pH 9.5, 23°C Cystobacter fuscus
1.4.1.28 4.89
-
 NADPH pH 9.5, 23°C Mycolicibacterium smegmatis
1.4.1.28 130
-
 (2R)-2-amino-4-oxopentanoate wild-type, pH 9.5, 23°C Petrotoga mobilis
1.4.1.28 1440
-
 NADH wild-type, pH 9.5, 23°C Petrotoga mobilis