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Literature summary extracted from

  • Faria, C.B.; de Castro, F.F.; Martim, D.B.; Abe, C.A.L.; Prates, K.V.; de Oliveira, M.A.S.; Barbosa-Tessmann, I.P.
    Production of galactose oxidase inside the Fusarium fujikuroi species complex and recombinant expression and characterization of the galactose oxidase GaoA protein from Fusarium subglutinans (2019), Mol. Biotechnol., 61, 633-649 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.9 glycerol 25%, 774% of initial activity Fusarium subglutinans
1.1.3.9 Tween 80 2.5%, 316% of initial activity Fusarium subglutinans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.9 expression in Escherichia coli Fusarium subglutinans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.9 CTAB 5 mM, 77% of initial activity Fusarium subglutinans
1.1.3.9 EDTA 5 mM, 81% of initial activity Fusarium subglutinans
1.1.3.9 KCl 5 mM, 90% of initial activity Fusarium subglutinans
1.1.3.9 NaCl 5 mM, 90% of initial activity Fusarium subglutinans
1.1.3.9 SDS 5 mM, 4% of initial activity Fusarium subglutinans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.9 132.6
-
 D-galactose pH 7, 30°C Fusarium subglutinans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.9 extracellular protein is secreted, and sequence presents N-terminal secretory and maturation sequences Fusarium subglutinans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.9 Mg2+ 5 mM, 107% of initial activity Fusarium subglutinans

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.3.9 Glycerol 25%, 774% of initial activity Fusarium subglutinans

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.9 Fusarium subglutinans A0A0U1YLU5
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.9 no glycoprotein sequence contains no putative N-glycosylation sites Fusarium subglutinans

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.9 recombinant protein Fusarium subglutinans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.9 alpha-D-melibiose + O2
-
 Fusarium subglutinans ? + H2O2
-
 ?
1.1.3.9 D-galactose + O2
-
 Fusarium subglutinans D-galacto-hexodialdose + H2O2
-
 ?
1.1.3.9 D-raffinose + O2
-
 Fusarium subglutinans ? + H2O2
-
 ?
1.1.3.9 methyl alpha-D-galactopyranoside + O2
-
 Fusarium subglutinans methyl alpha-D-galacto-hexodialdo-1,5-pyranoside + H2O2
-
 ?
1.1.3.9 additional information no substrate: D-glucose Fusarium subglutinans ?
-
-

Subunits

EC Number Subunits Comment Organism
1.1.3.9 ? x * 77000 and x * 74000, SDS-PAGE, x * 72500, calculated from sequence Fusarium subglutinans

Synonyms

EC Number Synonyms Comment Organism
1.1.3.9 GAOA
-
 Fusarium subglutinans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.9 30
-
-
 Fusarium subglutinans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.9 12243
-
 D-galactose pH 7, 30°C Fusarium subglutinans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.9 7
-
-
 Fusarium subglutinans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.3.9 Fusarium subglutinans calculated from sequence, mature protein
-
 7.6

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.9 92
-
 D-galactose pH 7, 30°C Fusarium subglutinans