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Literature summary extracted from

  • Forlani, G.; Nocek, B.; Ruszkowski, M.
    Peculiar substrate specificity of delta1-pyrroline-5-carboxylate reductase in the obligately fermentative bacterium Zymomonas mobilis (2021), Mol. Biol. Rep., 48, 6205-6211 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.2 expression in Escherichia coli Zymomonas mobilis subsp. mobilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.2 L-proline
-
 Zymomonas mobilis subsp. mobilis
1.5.1.2 NaCl
-
 Zymomonas mobilis subsp. mobilis
1.5.1.2 NAD+
-
 Zymomonas mobilis subsp. mobilis
1.5.1.2 NADP+
-
 Zymomonas mobilis subsp. mobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.2 0.135
-
 NADH pH 7.5, 30°C Zymomonas mobilis subsp. mobilis
1.5.1.2 0.273
-
 1-pyrroline-5-carboxylate cosubstrate NADH, pH 7.5, 30°C Zymomonas mobilis subsp. mobilis
1.5.1.2 0.719
-
 NADPH pH 7.5, 30°C Zymomonas mobilis subsp. mobilis
1.5.1.2 1.053
-
 1-pyrroline-5-carboxylate cosubstrate NADPH, pH 7.5, 30°C Zymomonas mobilis subsp. mobilis

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Zymomonas mobilis subsp. mobilis Q5NQR9
-
-
1.5.1.2 Zymomonas mobilis subsp. mobilis ATCC 31821 Q5NQR9
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.2 9.3
-
 substrate NADPH, pH 7.5, 30°C Zymomonas mobilis subsp. mobilis
1.5.1.2 36.53
-
 substrate NADH, pH 7.5, 30°C Zymomonas mobilis subsp. mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NADH + H+
-
 Zymomonas mobilis subsp. mobilis L-proline + NAD+
-
 ?
1.5.1.2 1-pyrroline-5-carboxylate + NADH + H+
-
 Zymomonas mobilis subsp. mobilis ATCC 31821 L-proline + NAD+
-
 ?
1.5.1.2 1-pyrroline-5-carboxylate + NADPH + H+
-
 Zymomonas mobilis subsp. mobilis L-proline + NADP+
-
 ?
1.5.1.2 1-pyrroline-5-carboxylate + NADPH + H+
-
 Zymomonas mobilis subsp. mobilis ATCC 31821 L-proline + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.2 PROC
-
 Zymomonas mobilis subsp. mobilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.2 6
-
 NADPH pH 7.5, 30°C Zymomonas mobilis subsp. mobilis
1.5.1.2 27
-
 NADH pH 7.5, 30°C Zymomonas mobilis subsp. mobilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.2 6.5
-
 and 7.7 Zymomonas mobilis subsp. mobilis
1.5.1.2 7.6
-
 and 6.5 Zymomonas mobilis subsp. mobilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADH maximal activity is evident with cofactor NADH Zymomonas mobilis subsp. mobilis
1.5.1.2 NADPH higher affinity for NADPH than NADH Zymomonas mobilis subsp. mobilis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.5.1.2 1.4
-
 substrate NADPH, pH 7.5, 30°C Zymomonas mobilis subsp. mobilis NAD+
1.5.1.2 7.4
-
 substrate NADH, pH 7.5, 30°C Zymomonas mobilis subsp. mobilis NAD+
1.5.1.2 22.6
-
 substrate NADH, pH 7.5, 30°C Zymomonas mobilis subsp. mobilis NADP+
1.5.1.2 96
-
 substrate NADH, pH 7.5, 30°C Zymomonas mobilis subsp. mobilis L-proline
1.5.1.2 107
-
 substrate NADPH, pH 7.5, 30°C Zymomonas mobilis subsp. mobilis L-proline
1.5.1.2 130
-
 substrate NADPH, pH 7.5, 30°C Zymomonas mobilis subsp. mobilis NaCl
1.5.1.2 1144
-
 substrate NADH, pH 7.5, 30°C Zymomonas mobilis subsp. mobilis NaCl

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.1.2 8.4
-
 NADPH pH 7.5, 30°C Zymomonas mobilis subsp. mobilis
1.5.1.2 196
-
 NADH pH 7.5, 30°C Zymomonas mobilis subsp. mobilis