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Literature summary extracted from

  • Liu, X.; Dong, Y.; Zhang, J.; Zhang, A.; Wang, L.; Feng, L.
    Two novel metal-independent long-chain alkyl alcohol dehydrogenases from Geobacillus thermodenitrificans NG80-2 (2009), Microbiology, 155, 2078-2085 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.1 EDTA 1 mM, 105.7% of initial activity Geobacillus thermodenitrificans
1.1.1.2 EDTA 1 mM, 109.1% of initial activity Geobacillus thermodenitrificans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expression in Escherichia coli Geobacillus thermodenitrificans
1.1.1.2 expression in Escherichia coli Geobacillus thermodenitrificans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 Al3+ 1 mM, 18.9% of initial activity Geobacillus thermodenitrificans
1.1.1.1 Ca2+ 1 mM, 98.4% of initial activity Geobacillus thermodenitrificans
1.1.1.1 Co2+ 1 mM, 51.9% of initial activity Geobacillus thermodenitrificans
1.1.1.1 Cu2+ 1 mM, 44.2% of initial activity Geobacillus thermodenitrificans
1.1.1.1 K+ 1 mM, 70.3% of initial activity Geobacillus thermodenitrificans
1.1.1.1 Mg2+ 1 mM, 74.35% of initial activity Geobacillus thermodenitrificans
1.1.1.1 Mn2+ 1 mM, 14.3% of initial activity Geobacillus thermodenitrificans
1.1.1.1 Ni2+ 1 mM, 72.7% of initial activity Geobacillus thermodenitrificans
1.1.1.1 SDS 1 mM, 2.9% of initial activity Geobacillus thermodenitrificans
1.1.1.1 Zn2+ 1 mM, 30.5% of initial activity Geobacillus thermodenitrificans
1.1.1.2 Al3+ 1 mM, 14.7% of initial activity Geobacillus thermodenitrificans
1.1.1.2 Ca2+ 1 mM, 0% of initial activity Geobacillus thermodenitrificans
1.1.1.2 Co2+ 1 mM, 68.7% of initial activity Geobacillus thermodenitrificans
1.1.1.2 Cu2+ 1 mM, 14.0% of initial activity Geobacillus thermodenitrificans
1.1.1.2 K+ 1 mM, 82.3% of initial activity Geobacillus thermodenitrificans
1.1.1.2 Mg2+ 1 mM, 0.3% of initial activity Geobacillus thermodenitrificans
1.1.1.2 Mn2+ 1 mM, 23.4% of initial activity Geobacillus thermodenitrificans
1.1.1.2 Ni2+ 1 mM, 18.2% of initial activity Geobacillus thermodenitrificans
1.1.1.2 SDS 1 mM,0% of initial activity Geobacillus thermodenitrificans
1.1.1.2 Zn2+ 1 mM, 14.1% of initial activity Geobacillus thermodenitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.28
-
 NADP+ cosubstrate ethanol, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.1 1.4
-
 NADH cosubstrate acetaldehyde, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.1 1.51
-
 NAD+ cosubstrate ethanol, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.1 4.71
-
 acetaldehyde pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.1 7.55
-
 ethanol pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 0.84
-
 NADH cosubstrate octanal, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 1.15
-
 octanal pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 1.44
-
 NAD+ cosubstrate octan-1-ol, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 2.25
-
 acetaldehyde pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 2.4
-
 NADP+ cosubstrate octan-1-ol, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 3.88
-
 octan-1-ol pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 37.6
-
 ethanol pH 8, 60°C Geobacillus thermodenitrificans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Fe2+ 1 mM, 136.1% of initial activity Geobacillus thermodenitrificans
1.1.1.1 additional information purified enzyme does not contain a significant amount of Fe, Ca, Co, Cu, Mg, Mn or Zn Geobacillus thermodenitrificans
1.1.1.1 Na+ 1 mM, 110.5% of initial activity Geobacillus thermodenitrificans
1.1.1.2 Fe2+ 1 mM, 168.9% of initial activity Geobacillus thermodenitrificans
1.1.1.2 additional information purified enzyme does not contain a significant amount of Fe, Ca, Co, Cu, Mg, Mn or Zn Geobacillus thermodenitrificans
1.1.1.2 Na+ 1 mM, 103.8% of initial activity Geobacillus thermodenitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 380000
-
 PAGE Geobacillus thermodenitrificans
1.1.1.2 90000
-
 PAGE Geobacillus thermodenitrificans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Geobacillus thermodenitrificans A4IP64 cf. EC 1.1.1.6 and EC 1.1.1.192
-
1.1.1.1 Geobacillus thermodenitrificans NG80-2 A4IP64 cf. EC 1.1.1.6 and EC 1.1.1.192
-
1.1.1.2 Geobacillus thermodenitrificans A4ISB9 cf. EC 1.1.1.192
-
1.1.1.2 Geobacillus thermodenitrificans NG80-2 A4ISB9 cf. EC 1.1.1.192
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1 recombinant enzyme, purification by nickel ion affinity chromatography Geobacillus thermodenitrificans
1.1.1.2 recombinant enzyme, purification by nickel ion affinity chromatography Geobacillus thermodenitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 447.6
-
 substrate ethanol, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 363.6
-
 substrate octan-1-ol, cosubstrate NADP+, pH 8, 60°C Geobacillus thermodenitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 1,3-propanediol + 2 NAD+ + H2O
-
 Geobacillus thermodenitrificans propanedial + 2 NADH + 2 H+
-
 r
1.1.1.1 acetaldehyde + NADH + H+
-
 Geobacillus thermodenitrificans ethanol + NAD+
-
 r
1.1.1.1 acetaldehyde + NADH + H+
-
 Geobacillus thermodenitrificans NG80-2 ethanol + NAD+
-
 r
1.1.1.1 butan-1-ol + NAD+
-
 Geobacillus thermodenitrificans butanal + NADH + H+
-
 r
1.1.1.1 decan-1-ol + NAD+
-
 Geobacillus thermodenitrificans decanal + NADH + H+
-
 r
1.1.1.1 dodecan-1-ol + NAD+
-
 Geobacillus thermodenitrificans dodecanal + NADH + H+
-
 r
1.1.1.1 eicosan-1-ol + NAD+
-
 Geobacillus thermodenitrificans n-eicosanal + NADH + H+
-
 r
1.1.1.1 ethanol + NAD+ ethanol is the best substrate Geobacillus thermodenitrificans acetaldehyde + NADH + H+
-
 r
1.1.1.1 ethanol + NADP+
-
 Geobacillus thermodenitrificans acetaldehyde + NADPH + H+
-
 r
1.1.1.1 ethanol + NADP+
-
 Geobacillus thermodenitrificans NG80-2 acetaldehyde + NADPH + H+
-
 r
1.1.1.1 formaldehyde + NADH + H+
-
 Geobacillus thermodenitrificans methanol + NAD+
-
 r
1.1.1.1 formaldehyde + NADH + H+
-
 Geobacillus thermodenitrificans NG80-2 methanol + NAD+
-
 r
1.1.1.1 glycerol + NAD+
-
 Geobacillus thermodenitrificans dihydroxyacetone + NADH + H+
-
 r
1.1.1.1 glycerol + NAD+
-
 Geobacillus thermodenitrificans NG80-2 dihydroxyacetone + NADH + H+
-
 r
1.1.1.1 hexadecan-1-ol + NAD+
-
 Geobacillus thermodenitrificans hexadecanal + NADH + H+
-
 r
1.1.1.1 hexan-1-ol + NAD+
-
 Geobacillus thermodenitrificans hexanal + NADH + H+
-
 r
1.1.1.1 isoamylalcohol + NAD+
-
 Geobacillus thermodenitrificans 3-methylbutanal + NADH + H+
-
 r
1.1.1.1 isopropanol + NAD+
-
 Geobacillus thermodenitrificans propan-2-one + NADH + H+
-
 r
1.1.1.1 methanol + NAD+
-
 Geobacillus thermodenitrificans formaldehyde + NADH + H+
-
 r
1.1.1.1 methanol + NAD+
-
 Geobacillus thermodenitrificans NG80-2 formaldehyde + NADH + H+
-
 r
1.1.1.1 octacosan-1-ol + NAD+
-
 Geobacillus thermodenitrificans octacosanal + NADH + H+
-
 r
1.1.1.1 octadecan-1-ol + NAD+
-
 Geobacillus thermodenitrificans octadecanal + NADH + H+
-
 r
1.1.1.1 octan-1-ol + NAD+
-
 Geobacillus thermodenitrificans octanal + NADH + H+
-
 r
1.1.1.1 tetracosanol-1-ol + NAD+
-
 Geobacillus thermodenitrificans tetracosanal + NADH + H+
-
 r
1.1.1.1 tetradecan-1-ol + NAD+
-
 Geobacillus thermodenitrificans tetradecanal + NADH + H+
-
 r
1.1.1.1 triacontan-1-ol + NAD+
-
 Geobacillus thermodenitrificans triacontanal + NADH + H+
-
 r
1.1.1.2 1,3-propanediol + 2 NADP+ + H2O
-
 Geobacillus thermodenitrificans propanedial + 2 NADPH + 2 H+
-
 r
1.1.1.2 acetaldehyde + NADPH + H+
-
 Geobacillus thermodenitrificans ethanol + NADP+
-
 r
1.1.1.2 acetaldehyde + NADPH + H+
-
 Geobacillus thermodenitrificans NG80-2 ethanol + NADP+
-
 r
1.1.1.2 acetone + NADP+
-
 Geobacillus thermodenitrificans propan-2-ol + NADPH + H+
-
 r
1.1.1.2 butan-1-ol + NADP+
-
 Geobacillus thermodenitrificans butanal + NADPH + H+
-
 r
1.1.1.2 butan-1-ol + NADP+
-
 Geobacillus thermodenitrificans NG80-2 butanal + NADPH + H+
-
 r
1.1.1.2 decan-1-ol + NADP+
-
 Geobacillus thermodenitrificans decanal + NADPH + H+
-
 r
1.1.1.2 decanal + NADP+
-
 Geobacillus thermodenitrificans decan-1-ol + NADPH + H+
-
 r
1.1.1.2 dodecan-1-ol + NADP+
-
 Geobacillus thermodenitrificans dodecanal + NADPH + H+
-
 r
1.1.1.2 dodecanal + NADP+
-
 Geobacillus thermodenitrificans dodecan-1-ol + NADPH + H+
-
 r
1.1.1.2 eicosan-1-ol + NADP+
-
 Geobacillus thermodenitrificans n-eicosanal + NADPH + H+
-
 r
1.1.1.2 ethanol + NADP+ ethanol is the best substrate Geobacillus thermodenitrificans acetaldehyde + NADPH + H+
-
 r
1.1.1.2 ethanol + NADP+ ethanol is the best substrate Geobacillus thermodenitrificans NG80-2 acetaldehyde + NADPH + H+
-
 r
1.1.1.2 formaldehyde + NADPH + H+
-
 Geobacillus thermodenitrificans methanol + NADP+
-
 r
1.1.1.2 glycerol + NADP+
-
 Geobacillus thermodenitrificans dihydroxyacetone + NADPH + H+
-
 r
1.1.1.2 glycerol + NADP+
-
 Geobacillus thermodenitrificans NG80-2 dihydroxyacetone + NADPH + H+
-
 r
1.1.1.2 hexadecan-1-ol + NADP+
-
 Geobacillus thermodenitrificans hexadecanal + NADPH + H+
-
 r
1.1.1.2 hexan-1-ol + NADP+
-
 Geobacillus thermodenitrificans hexanal + NADPH + H+
-
 r
1.1.1.2 isoamylalcohol + NADP+
-
 Geobacillus thermodenitrificans 3-methylbutanal + NADPH + H+
-
 r
1.1.1.2 isopropanol + NADP+
-
 Geobacillus thermodenitrificans propan-2-one + NADPH + H+
-
 r
1.1.1.2 methanol + NAD+
-
 Geobacillus thermodenitrificans formaldehyde + NADH + H+
-
 r
1.1.1.2 methanol + NAD+
-
 Geobacillus thermodenitrificans NG80-2 formaldehyde + NADH + H+
-
 r
1.1.1.2 octacosan-1-ol + NADP+
-
 Geobacillus thermodenitrificans octacosanal + NADPH + H+
-
 r
1.1.1.2 octadecan-1-ol + NADP+
-
 Geobacillus thermodenitrificans octadecanal + NADPH + H+
-
 r
1.1.1.2 octan-1-ol + NADP+
-
 Geobacillus thermodenitrificans octanal + NADPH + H+
-
 r
1.1.1.2 octanal + NADP+
-
 Geobacillus thermodenitrificans octan-1-ol + NADPH + H+
-
 r
1.1.1.2 tetracosanol-1-ol + NADP+
-
 Geobacillus thermodenitrificans tetracosanal + NADPH + H+
-
 r
1.1.1.2 tetradecan-1-ol + NADP+
-
 Geobacillus thermodenitrificans tetradecanal + NADPH + H+
-
 r
1.1.1.2 triacontan-1-ol + NADP+
-
 Geobacillus thermodenitrificans triacontanal + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.1 octamer 8 * 45000, SDS-PAGE, 8 * 41664, calculated from sequence Geobacillus thermodenitrificans
1.1.1.2 dimer 2 * 45000, SDS-PAGE, 2 * 42804, calculated from sequence Geobacillus thermodenitrificans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 ADH1
-
 Geobacillus thermodenitrificans
1.1.1.2 ADH2
-
 Geobacillus thermodenitrificans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 60
-
-
 Geobacillus thermodenitrificans
1.1.1.2 60
-
-
 Geobacillus thermodenitrificans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.1 45 75
-
 Geobacillus thermodenitrificans
1.1.1.2 50 65
-
 Geobacillus thermodenitrificans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 60
-
 14 h, more than 50% of initial activity Geobacillus thermodenitrificans
1.1.1.2 60
-
 14 h, more than 50% of initial activity Geobacillus thermodenitrificans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 43.9
-
 NADP+ cosubstrate ethanol, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.1 404.8
-
 acetaldehyde pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.1 443.1
-
 NAD+ cosubstrate ethanol, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.1 638.7
-
 ethanol pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.1 1645.7
-
 NADH cosubstrate acetaldehyde, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 38.5
-
 NAD+ cosubstrate octan-1-ol, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 136.2
-
 ethanol pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 138.1
-
 acetaldehyde pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 260.7
-
 octan-1-ol pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 283.1
-
 NADP+ cosubstrate octan-1-ol, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 518.4
-
 octanal pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 4808.2
-
 NADH cosubstrate octanal, pH 8, 60°C Geobacillus thermodenitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 8
-
-
 Geobacillus thermodenitrificans
1.1.1.2 8
-
-
 Geobacillus thermodenitrificans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.1 6 9
-
 Geobacillus thermodenitrificans
1.1.1.2 6 9
-
 Geobacillus thermodenitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+ NAD+ is about 2fold preferred over NADP+ Geobacillus thermodenitrificans
1.1.1.1 NADH
-
 Geobacillus thermodenitrificans
1.1.1.1 NADP+ NAD+ is about 2fold preferred over NADP+ Geobacillus thermodenitrificans
1.1.1.2 NAD+ NADP+ is about 4fold preferred over NADP+ Geobacillus thermodenitrificans
1.1.1.2 NADP+ NADP+ is about 4fold preferred over NADP+ Geobacillus thermodenitrificans
1.1.1.2 NADPH
-
 Geobacillus thermodenitrificans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.1 84.6
-
 ethanol pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.1 85.9
-
 acetaldehyde pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.1 156.9
-
 NADP+ cosubstrate ethanol, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.1 293.4
-
 NAD+ cosubstrate ethanol, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.1 1175.5
-
 NADH cosubstrate acetaldehyde, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 3.62
-
 ethanol pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 26.7
-
 NAD+ cosubstrate octan-1-ol, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 61.4
-
 acetaldehyde pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 67.2
-
 octan-1-ol pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 119.9
-
 NADP+ cosubstrate octan-1-ol, pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 450.8
-
 octanal pH 8, 60°C Geobacillus thermodenitrificans
1.1.1.2 5724
-
 NADH cosubstrate octanal, pH 8, 60°C Geobacillus thermodenitrificans